FastQCFastQC Report
Thu 26 May 2016
SRR1546406_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546406_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences667265
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22420.33599844139884455No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17080.25597026668564965No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15930.2387357346781264No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9260.13877544903449154No Hit
AAGGAAAGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8130.1218406480184035No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7310.10955167736956081No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGT207.031158E-445.034
ATTTACG207.031158E-445.01
AGTCCAT207.031158E-445.044
ATTAGCC406.8084773E-945.012
CATGCGC207.031158E-445.027
CGTGCGG253.8890415E-545.02
GGCCGTA253.8890415E-545.040
CCTGTCG207.031158E-445.019
ACGGGTA207.031158E-445.05
ATCGTAG207.031158E-445.045
AAAGTCG207.031158E-445.039
ACGATCT253.8890415E-545.016
CTAACGG207.031158E-445.02
CCCCGAC207.031158E-445.025
GAGCGTA207.031158E-445.015
TTAAGCC253.8890415E-545.032
CGTAGAT207.031158E-445.013
GAACGTC207.031158E-445.015
CATACTC207.031158E-445.014
TCGAGGA253.8890415E-545.04