##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546406_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	667265
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.014087356597454	31.0	31.0	33.0	30.0	33.0
2	31.165713771889727	31.0	31.0	33.0	30.0	34.0
3	31.118660502199276	31.0	31.0	34.0	28.0	34.0
4	35.0924864933722	35.0	35.0	37.0	32.0	37.0
5	32.79933759450893	35.0	35.0	37.0	28.0	37.0
6	33.9791881786097	35.0	35.0	37.0	28.0	37.0
7	15.309755494443737	0.0	0.0	35.0	0.0	37.0
8	24.908123459195373	17.0	17.0	35.0	17.0	37.0
9	32.64386862790646	32.0	32.0	37.0	27.0	39.0
10	35.526266176106944	37.0	35.0	37.0	32.0	39.0
11	36.45923283852742	37.0	35.0	39.0	32.0	39.0
12	36.19653510973901	37.0	35.0	39.0	32.0	39.0
13	35.726960053352116	37.0	35.0	39.0	31.0	39.0
14	36.72983447355998	39.0	35.0	40.0	31.0	41.0
15	37.17814661341446	39.0	35.0	41.0	32.0	41.0
16	37.27682405041475	39.0	35.0	41.0	32.0	41.0
17	37.26739751073411	39.0	35.0	41.0	32.0	41.0
18	37.17004488471597	39.0	35.0	40.0	32.0	41.0
19	37.081694679025574	39.0	35.0	40.0	32.0	41.0
20	37.195448584895054	39.0	35.0	40.0	32.0	41.0
21	37.008982937813315	39.0	35.0	40.0	32.0	41.0
22	37.058270702044915	39.0	35.0	40.0	32.0	41.0
23	37.061689134002236	39.0	35.0	40.0	32.0	41.0
24	36.9018860572636	38.0	35.0	40.0	32.0	41.0
25	36.63657017826501	38.0	35.0	40.0	31.0	41.0
26	36.49040935760155	38.0	35.0	40.0	31.0	41.0
27	36.37906978486808	38.0	35.0	40.0	31.0	41.0
28	36.31509070609128	38.0	35.0	40.0	31.0	41.0
29	36.37885847451912	38.0	35.0	40.0	31.0	41.0
30	36.141669351756796	38.0	35.0	40.0	30.0	41.0
31	36.01414730279574	38.0	35.0	40.0	30.0	41.0
32	35.88449116917567	38.0	34.0	40.0	30.0	41.0
33	35.684065551167826	38.0	34.0	40.0	29.0	41.0
34	35.530974949982394	38.0	34.0	40.0	29.0	41.0
35	35.37129925891512	38.0	34.0	40.0	27.0	41.0
36	35.13514121076334	38.0	34.0	40.0	26.0	41.0
37	34.94479854330738	38.0	34.0	40.0	25.0	41.0
38	34.888856751065916	38.0	34.0	40.0	25.0	41.0
39	34.82444306235154	38.0	34.0	40.0	25.0	41.0
40	34.64344600720853	37.0	33.0	40.0	24.0	41.0
41	34.53784628296104	37.0	33.0	40.0	24.0	41.0
42	34.484988722621445	37.0	33.0	40.0	23.0	41.0
43	34.39492105835013	37.0	33.0	40.0	23.0	41.0
44	34.1772983747087	37.0	33.0	40.0	23.0	41.0
45	34.09859501097765	36.0	33.0	40.0	23.0	41.0
46	34.06975789229167	36.0	33.0	40.0	23.0	41.0
47	34.03508501120245	36.0	33.0	40.0	23.0	41.0
48	34.02683041969832	36.0	33.0	40.0	23.0	41.0
49	33.950569863547464	36.0	33.0	40.0	23.0	41.0
50	33.8427401407237	36.0	33.0	40.0	23.0	41.0
51	33.63603965441017	36.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	3.0
14	13.0
15	26.0
16	90.0
17	223.0
18	476.0
19	885.0
20	1375.0
21	2374.0
22	3582.0
23	5083.0
24	7312.0
25	10022.0
26	11899.0
27	13107.0
28	13985.0
29	16277.0
30	19589.0
31	25924.0
32	34759.0
33	52872.0
34	67392.0
35	67445.0
36	74490.0
37	113634.0
38	104570.0
39	19855.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.485833964017292	25.662817621184985	30.22832008272575	15.623028332071964
2	30.827332469108974	25.0853858661851	31.23511648295655	12.852165181749381
3	29.022502304181998	24.004106314582664	33.906618809618365	13.066772571616974
4	26.872681768113114	26.89441226499217	31.079556098401685	15.153349868493027
5	21.312372145998964	37.244123399249176	28.512659887750747	12.930844567001115
6	21.41607906903554	40.42217110143646	27.31763242489865	10.844117404629346
7	37.54490344915438	57.268101878563996	4.3981027028242154	0.788891969457412
8	87.22621447251092	4.044420132930695	7.151431590147843	1.5779338044105413
9	82.29024450555626	6.082890605681401	8.892868650386278	2.7339962383760574
10	55.90117869212382	20.901890553228476	13.503330760642324	9.69359999400538
11	51.06337062486418	17.91806853349119	19.364570298157403	11.65399054348722
12	44.1373367402756	21.21675795973114	22.825713921755224	11.820191378238032
13	21.38985260728496	43.14327890718081	22.883861734093653	12.583006751440582
14	14.53410564018793	44.682997010183364	27.44741594419009	13.335481405438617
15	14.111484942264319	22.309876885495267	50.017609195746815	13.561028976493597
16	15.111237664196384	17.351726825174406	48.156129873438594	19.380905637190622
17	15.861314470262938	18.062838602354386	29.27652431942332	36.799322607959354
18	21.313720935460424	22.832757600053952	34.71379436955333	21.139727094932297
19	29.905060208462903	22.811926296149206	26.025492120821564	21.257521374566327
20	31.688609472997985	22.68543981776356	25.234951630911258	20.3909990783272
21	21.813822094670034	27.66097427558766	28.6460401789394	21.879163450802906
22	22.562250380283697	25.263126344106162	25.710774579814615	26.463848695795523
23	20.161704869879284	29.677864116955032	25.27901208665223	24.881418926513454
24	19.599409529946872	23.00600211310349	39.86077495447836	17.53381340247128
25	16.832442882512943	24.750736214247713	37.44689141495508	20.969929488284265
26	16.54215341730797	34.486148681558305	28.35919762013593	20.612500280997807
27	18.13312551984594	33.8583621199973	29.53174525863038	18.47676710152638
28	15.02941110353458	28.906206679505143	39.31556428105775	16.748817935902526
29	14.615632469858303	24.839606453208248	38.88964654222835	21.655114534705103
30	18.074378245524642	30.682262669254346	32.02640630034544	19.216952784875573
31	29.633204199231187	26.53803211617573	25.275715045746445	18.553048638846636
32	30.690954868005964	26.41619146815733	26.200235288828278	16.69261837500843
33	27.332169377983263	27.622908439675392	25.75528463204274	19.289637550298607
34	19.155058335144208	27.97059638974021	29.06836114587158	23.805984129244003
35	21.122042966437622	24.781908237356973	31.08240354282032	23.013645253385086
36	30.246004211220427	24.987373831985792	26.29794759203615	18.468674364757632
37	20.04301139727095	33.310903464140935	28.677062336552943	17.969022802035173
38	20.04241193528808	33.87095082163758	24.25962698478116	21.827010258293182
39	21.323012596194914	32.605036979311066	26.37377953286925	19.698170891624766
40	23.84285104119053	26.970094340329553	24.564453403070743	24.62260121540917
41	18.065386315781588	24.63623897551947	26.14133814901126	31.15703655968768
42	21.784148726517948	26.25538579125235	24.03662712715338	27.92383835507632
43	22.947854300765062	26.35999190726323	25.568402358882903	25.123751433088803
44	19.714506230657985	30.976748368339415	27.80844192337377	21.50030347762883
45	17.362217409874635	38.12203547316283	22.25232853513971	22.263418581822812
46	21.351786771372694	34.0304077090811	25.575146306190195	19.042659213356014
47	22.68543981776356	27.16761706368534	27.44846500266011	22.69847811589099
48	23.085730556825247	24.464043520939956	30.215581515589758	22.234644406645035
49	20.2589675766	24.723760425018547	32.20055000636928	22.81672199201217
50	19.50304601620046	33.97136070376837	26.23785152825339	20.28774175177778
51	18.98496099750474	34.149850509168026	23.515095202056155	23.350093291271083
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	336.0
1	541.0
2	746.0
3	3456.0
4	6166.0
5	4756.5
6	3347.0
7	3440.0
8	3533.0
9	3615.5
10	3698.0
11	3695.0
12	3692.0
13	3591.0
14	3490.0
15	3296.5
16	3103.0
17	2921.0
18	2739.0
19	2806.5
20	2874.0
21	2845.5
22	2817.0
23	2999.0
24	3181.0
25	4046.0
26	5835.5
27	6760.0
28	8731.0
29	10702.0
30	11802.0
31	12902.0
32	13635.5
33	14369.0
34	15571.0
35	16773.0
36	19582.5
37	22392.0
38	30584.0
39	38776.0
40	49154.5
41	59533.0
42	64993.0
43	70453.0
44	67709.0
45	64965.0
46	60742.5
47	56520.0
48	52136.5
49	47753.0
50	43864.5
51	39976.0
52	36726.0
53	33476.0
54	30309.5
55	27143.0
56	25408.0
57	23673.0
58	22017.5
59	20362.0
60	18961.5
61	17561.0
62	15064.5
63	12568.0
64	10567.5
65	8567.0
66	7364.5
67	6162.0
68	5141.5
69	4121.0
70	3460.0
71	2799.0
72	2239.0
73	1679.0
74	1413.0
75	917.0
76	687.0
77	527.0
78	367.0
79	313.5
80	260.0
81	195.0
82	130.0
83	81.5
84	33.0
85	21.5
86	10.0
87	8.5
88	7.0
89	5.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	667265.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.602609806887116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95512516906079	17.691365723917258
2	5.5006362276722305	2.596587411427503
3	2.561483722750994	1.8137310250435301
4	1.6829162264353135	1.5888486012092635
5	1.2453159178691613	1.469635284788565
6	1.0429185926003641	1.4769360360896562
7	0.8737511291442087	1.4435964878662457
8	0.7574140772560883	1.4301559142175126
9	0.6862537403111333	1.4577641334972467
>10	9.853276593931929	54.29819911132998
>50	0.779625256245095	11.39337906698454
>100	0.05622905008574834	1.962694264506911
>500	0.0031589353980757497	0.550544539170302
>1k	0.00189536123884545	0.8265623999515125
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2242	0.33599844139884455	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1708	0.25597026668564965	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1593	0.2387357346781264	No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	926	0.13877544903449154	No Hit
AAGGAAAGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	813	0.1218406480184035	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	731	0.10955167736956081	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004795695862962991	0.0
2	0.0	0.0	0.0	0.03282054356215297	0.0
3	0.0	0.0	0.0	0.046608169168171565	0.0
4	0.0	0.0	0.0	0.07718073029456063	0.0
5	0.0	0.0	0.0	0.12843472982997758	0.0
6	0.0	0.0	0.0	0.18508388721122793	0.0
7	0.0	0.0	0.0	0.21865375825196887	0.0
8	0.0	0.0	0.0	0.4624849197844934	0.0
9	0.0	0.0	0.0	0.5449109424291698	0.0
10	0.0	0.0	0.0	0.6778416371306752	0.0
11	0.0	0.0	0.0	0.8287561913182918	0.0
12	0.0	0.0	0.0	0.9540437457381999	0.0
13	0.0	0.0	0.0	1.007545727709381	0.0
14	0.0	0.0	0.0	1.0265786456655153	0.0
15	0.0	0.0	0.0	1.0553528208432932	0.0
16	0.0	0.0	0.0	1.135830592043641	0.0
17	0.0	0.0	0.0	1.232044240294336	0.0
18	0.0	0.0	0.0	1.3801113500633182	0.0
19	0.0	0.0	0.0	1.4441039167347305	0.0
20	0.0	0.0	0.0	1.508096483406143	0.0
21	0.0	0.0	0.0	1.6016125527339213	0.0
22	0.0	0.0	0.0	1.6843383063700328	0.0
23	0.0	0.0	0.0	1.7895438843637834	0.0
24	0.0	0.0	0.0	1.8743677549399413	0.0
25	0.0	0.0	0.0	1.9418072280128584	0.0
26	0.0	0.0	0.0	2.013143203974433	0.0
27	0.0	0.0	0.0	2.0678441099113547	0.0
28	0.0	0.0	0.0	2.1397795478557993	0.0
29	0.0	0.0	0.0	2.2067694244415637	0.0
30	0.0	0.0	0.0	2.308078499546657	0.0
31	0.0	0.0	0.0	2.404591878788787	0.0
32	0.0	0.0	0.0	2.492413059279297	0.0
33	0.0	0.0	0.0	2.5758881403939964	0.0
34	0.0	0.0	0.0	2.671502326661821	0.0
35	0.0	0.0	0.0	2.7873483548515208	0.0
36	0.0	0.0	0.0	2.8817636171536045	0.0
37	0.0	0.0	0.0	2.981124440814369	0.0
38	0.0	0.0	0.0	3.067896562834856	0.0
39	0.0	0.0	0.0	3.1531700298981664	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGT	20	7.031158E-4	45.0	34
ATTTACG	20	7.031158E-4	45.0	1
AGTCCAT	20	7.031158E-4	45.0	44
ATTAGCC	40	6.8084773E-9	45.0	12
CATGCGC	20	7.031158E-4	45.0	27
CGTGCGG	25	3.8890415E-5	45.0	2
GGCCGTA	25	3.8890415E-5	45.0	40
CCTGTCG	20	7.031158E-4	45.0	19
ACGGGTA	20	7.031158E-4	45.0	5
ATCGTAG	20	7.031158E-4	45.0	45
AAAGTCG	20	7.031158E-4	45.0	39
ACGATCT	25	3.8890415E-5	45.0	16
CTAACGG	20	7.031158E-4	45.0	2
CCCCGAC	20	7.031158E-4	45.0	25
GAGCGTA	20	7.031158E-4	45.0	15
TTAAGCC	25	3.8890415E-5	45.0	32
CGTAGAT	20	7.031158E-4	45.0	13
GAACGTC	20	7.031158E-4	45.0	15
CATACTC	20	7.031158E-4	45.0	14
TCGAGGA	25	3.8890415E-5	45.0	4
>>END_MODULE