FastQCFastQC Report
Thu 26 May 2016
SRR1546405_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546405_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2851659
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129130.4528241279900577No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA61560.21587433841142997No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55310.1939572718898017No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG207.858445E-444.00000428
GATACGA302.529041E-644.00000435
CTCGTCG207.858445E-444.00000430
CGTTACA302.529041E-644.00000425
TACGCAC207.858445E-444.00000414
TCGCTAA207.858445E-444.00000423
TCGACGA207.858445E-444.00000414
GACTCGA207.858445E-444.00000434
TCACGAA351.447097E-744.01
CCGGACT254.4445045E-544.038
ATCGCTT351.447097E-744.043
ATCGCTA551.8189894E-1244.01
TACCGGT502.7284841E-1144.041
CGTTTTA86650.043.6445471
ACATACG7850.042.87898617
CGTTATT46650.042.396571
AGGCGAT18900.041.788367
CTAACGG1650.041.3333322
GTTTTAT97950.041.2149052
TATTACG700.040.8571431
Produced by FastQC (version 0.11.5)