##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546405_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2851659
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.499005315852983	31.0	31.0	33.0	30.0	34.0
2	31.68245922811949	31.0	31.0	34.0	30.0	34.0
3	31.72204846371884	31.0	31.0	34.0	30.0	34.0
4	35.630937289486575	37.0	35.0	37.0	33.0	37.0
5	27.08641986997744	35.0	25.0	37.0	0.0	37.0
6	31.315750585887024	35.0	26.0	37.0	17.0	37.0
7	15.054063617003296	0.0	0.0	32.0	0.0	37.0
8	25.132805850909943	17.0	17.0	35.0	17.0	37.0
9	33.186310845721735	32.0	32.0	37.0	28.0	39.0
10	36.00065751199565	37.0	35.0	39.0	32.0	39.0
11	36.97314370336706	39.0	37.0	39.0	34.0	39.0
12	36.93938756352004	39.0	35.0	39.0	33.0	39.0
13	36.43357638483423	39.0	35.0	39.0	32.0	39.0
14	37.770019486902186	40.0	36.0	41.0	33.0	41.0
15	38.05654392758741	40.0	36.0	41.0	33.0	41.0
16	38.1243655009242	40.0	36.0	41.0	33.0	41.0
17	38.14167963280322	40.0	36.0	41.0	34.0	41.0
18	38.09810394580839	40.0	36.0	41.0	34.0	41.0
19	38.02456254411906	40.0	36.0	41.0	34.0	41.0
20	38.0312905575316	40.0	36.0	41.0	34.0	41.0
21	37.88582365563344	40.0	36.0	41.0	34.0	41.0
22	37.78931842832541	39.0	35.0	41.0	33.0	41.0
23	37.68654386797299	39.0	35.0	41.0	33.0	41.0
24	37.534569526019766	39.0	35.0	41.0	33.0	41.0
25	37.36204048239989	39.0	35.0	41.0	33.0	41.0
26	37.092710594078746	39.0	35.0	40.0	33.0	41.0
27	36.87975981700477	39.0	35.0	40.0	32.0	41.0
28	36.91033149475446	39.0	35.0	40.0	32.0	41.0
29	36.8964571149636	38.0	35.0	40.0	32.0	41.0
30	36.695454821211094	38.0	35.0	40.0	32.0	41.0
31	36.4911200813281	38.0	35.0	40.0	31.0	41.0
32	36.20122286710999	38.0	35.0	40.0	31.0	41.0
33	35.9608056222711	38.0	35.0	40.0	30.0	41.0
34	35.711110620168824	38.0	35.0	40.0	29.0	41.0
35	35.5043369491233	38.0	35.0	40.0	28.0	41.0
36	35.30098900324337	38.0	35.0	40.0	27.0	41.0
37	35.11917589024494	38.0	34.0	40.0	26.0	41.0
38	34.976588715551195	38.0	34.0	40.0	26.0	41.0
39	34.90463901890093	38.0	34.0	40.0	25.0	41.0
40	34.67389999996493	38.0	34.0	40.0	24.0	41.0
41	34.52030835383894	37.0	34.0	40.0	24.0	40.0
42	34.52279111913451	37.0	34.0	40.0	24.0	41.0
43	34.365107469020664	37.0	33.0	40.0	23.0	40.0
44	34.14906375551916	37.0	33.0	40.0	23.0	40.0
45	33.97335621124405	37.0	33.0	40.0	23.0	40.0
46	33.92926398282544	36.0	33.0	40.0	23.0	40.0
47	33.77650097715049	36.0	33.0	39.0	23.0	40.0
48	33.71599549595516	36.0	33.0	39.0	23.0	40.0
49	33.58325241552374	36.0	33.0	39.0	23.0	40.0
50	33.644880050524975	36.0	33.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	5.0
12	3.0
13	7.0
14	14.0
15	60.0
16	150.0
17	446.0
18	1088.0
19	2389.0
20	4240.0
21	7250.0
22	11770.0
23	18055.0
24	28002.0
25	40923.0
26	51641.0
27	54771.0
28	55635.0
29	60819.0
30	72730.0
31	95334.0
32	130841.0
33	236084.0
34	238263.0
35	288648.0
36	370118.0
37	685150.0
38	317655.0
39	79566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.67490152223671	22.734275030780328	30.161179860565372	17.42964358641759
2	31.538588590010235	24.7737194384041	30.341706354090725	13.345985617494938
3	27.957725660746956	22.8786471313716	35.91547937533905	13.24814783254239
4	26.032916277857908	26.23322073221237	33.225396164127616	14.508466825802103
5	16.95472705537373	47.51255321902092	24.94761821101331	10.585101514592033
6	20.699038699928707	39.28516698525314	29.79676041209696	10.219033902721188
7	38.07955299003142	55.74825040441371	5.09608617299614	1.0761104325587316
8	85.15863923421418	3.1293012243048692	9.736858439245367	1.975201102235576
9	80.50576874724501	5.08861683672557	11.568949863921317	2.8366645521080884
10	49.901864142942756	24.251391909060654	16.608753010089917	9.237990937906671
11	45.042622557605945	19.592209306933263	23.231108628345815	12.134059507114982
12	40.60972928390106	19.922262795095765	26.079696064641674	13.388311856361506
13	21.390601050125557	36.87814707158184	26.254787125669655	15.476464752622949
14	15.106644938963601	39.0090820816935	29.60420583246454	16.28006714687836
15	14.463019596662855	22.294425806171077	48.413502455938804	14.829052141227264
16	16.301493271109905	18.036939199252082	47.10349308946126	18.558074440176753
17	16.868110808480257	18.612358630537525	31.373421576703247	33.146108984278975
18	20.743504044487786	23.736709052519956	35.13249655726719	20.387290345725066
19	29.058488409729215	23.40939081425935	27.714288419477924	19.81783235653351
20	31.181147535522303	22.360001669203786	27.141428901562215	19.317421893711696
21	21.970719500473233	26.65118795760643	29.8952995431782	21.482792998742138
22	21.6473288005333	25.008249583838744	27.684165603250598	25.66025601237736
23	20.503082591572134	29.095414283404853	27.31234695312448	23.089156171898534
24	20.72523397783536	22.677465994356268	38.57326559732422	18.02403443048415
25	18.261510229659297	24.437108363938325	35.5692598589102	21.732121547492177
26	17.240806141267242	32.28625863050246	30.10409028568984	20.368844942540466
27	18.608185621071804	30.730918388208405	31.261662071096158	19.39923391962363
28	15.570655537706296	28.19800684443687	38.648274565787844	17.58306305206899
29	16.439483121930078	24.150187662690385	37.54070875935727	21.869620456022265
30	18.28300648850371	27.81910459841096	33.27115899902478	20.62672991406055
31	26.646488938544195	26.12766813984421	27.245263195915083	19.980579725696515
32	28.00699522628757	25.895136830876343	28.559971581454864	17.537896361381218
33	26.56657054717973	27.183334332751567	27.02595226147306	19.224142858595645
34	19.87453619103827	26.80913110578789	30.223739935244716	23.092592767929123
35	20.782849562307415	25.59026166873388	30.697779783627706	22.929108985330995
36	27.359687816811196	26.145833004577334	27.826679136600834	18.667800042010633
37	20.28503408016176	32.05071153318121	29.640044619640708	18.024209767016323
38	20.66190242241446	31.621592904340943	26.27400400959582	21.442500663648776
39	21.89234407059189	29.776561643590625	28.106130501578203	20.22496378423928
40	23.320530259754058	25.822407237330967	27.305719232208336	23.55134327070663
41	17.330438176514093	25.43067035715	28.366785790306626	28.872105676029285
42	21.917732800450544	26.37527839057896	24.94604018222375	26.760948626746746
43	22.917291303062534	26.637581842709807	26.48279475210746	23.962332102120204
44	19.820602673741845	30.794039539790695	27.5527333387337	21.83262444773376
45	18.0787043612157	35.06905979992699	23.333610365054167	23.518625473803144
46	20.682802537049486	32.21289782544126	26.84367240262598	20.260627234883273
47	22.61178492940425	26.528347183166012	27.965931410452654	22.893936476977085
48	23.14358063148504	22.95060524417541	30.543974577605525	23.36183954673402
49	20.20206483313748	24.293192138330706	32.19143663390328	23.31330639462853
50	18.740845241313917	31.34070377979976	28.20929851710881	21.70915246177751
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1412.0
1	2920.5
2	4429.0
3	22674.5
4	40920.0
5	30582.0
6	20244.0
7	20588.0
8	20932.0
9	21501.0
10	22070.0
11	21552.0
12	21034.0
13	20459.5
14	19885.0
15	19234.0
16	18583.0
17	17691.0
18	16799.0
19	15557.5
20	14316.0
21	14522.0
22	14728.0
23	16091.5
24	17455.0
25	22427.0
26	27399.0
27	31277.5
28	35156.0
29	41527.0
30	47898.0
31	52699.0
32	57500.0
33	63203.0
34	68906.0
35	74848.5
36	80791.0
37	94109.5
38	107428.0
39	140649.0
40	173870.0
41	210718.0
42	247566.0
43	258532.5
44	269499.0
45	260040.0
46	250581.0
47	239803.5
48	229026.0
49	216285.0
50	203544.0
51	185603.0
52	167662.0
53	151101.0
54	134540.0
55	124928.5
56	115317.0
57	111115.5
58	106914.0
59	104060.0
60	101206.0
61	89740.5
62	78275.0
63	61794.5
64	45314.0
65	35404.5
66	25495.0
67	21166.5
68	16838.0
69	14194.0
70	11550.0
71	9441.5
72	7333.0
73	5740.0
74	4147.0
75	3196.5
76	2246.0
77	1796.5
78	1347.0
79	1025.0
80	703.0
81	562.5
82	422.0
83	361.0
84	300.0
85	178.0
86	56.0
87	34.0
88	12.0
89	9.5
90	7.0
91	5.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2851659.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.16239013917743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.06124886545346	16.14226134655049
2	7.507331687327847	3.0273150076822644
3	2.4195315997604703	1.463506202053161
4	1.1528261002710516	0.9297491838516568
5	0.7014071316792629	0.7071022117659348
6	0.5032295538429659	0.6087786356487643
7	0.3995648963278575	0.5639328327977573
8	0.33204689223511524	0.535588718859664
9	0.2883558166615885	0.523254822698886
>10	3.9748800348980504	20.326462182093003
>50	1.6266921807635009	23.489796737013638
>100	1.0276218637313297	29.796417083557685
>500	0.003579096392300118	0.4926195377961496
>1k	0.0010526754095000345	0.34897244465507504
>5k	4.2107016380001387E-4	0.4961003479727359
>10k+	2.1053508190000693E-4	0.5481427050031606
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12913	0.4528241279900577	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6156	0.21587433841142997	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5531	0.1939572718898017	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02079491271572092	0.0
2	0.0	0.0	0.0	0.08107561247680736	0.0
3	0.0	0.0	0.0	0.10821770765719183	0.0
4	0.0	0.0	0.0	0.15625991747260104	0.0
5	0.0	0.0	0.0	0.24378791433337577	0.0
6	0.0	0.0	0.0	0.377464486462091	0.0
7	0.0	0.0	0.0	0.47972075202539993	0.0
8	0.0	0.0	0.0	0.9777115707032292	0.0
9	0.0	0.0	0.0	1.1463853146536804	0.0
10	0.0	0.0	0.0	1.4384959772539423	0.0
11	0.0	0.0	0.0	1.7644816578700329	0.0
12	0.0	0.0	0.0	2.046317599684955	0.0
13	0.0	0.0	0.0	2.1650905665789635	0.0
14	0.0	0.0	0.0	2.20625958433319	0.0
15	0.0	0.0	0.0	2.2766747356538772	0.0
16	0.0	0.0	0.0	2.437388201043673	0.0
17	0.0	0.0	0.0	2.6347470016576318	0.0
18	0.0	0.0	0.0	2.906834232283734	0.0
19	0.0	0.0	0.0	3.0313231701265826	0.0
20	0.0	0.0	0.0	3.1617384827568795	0.0
21	0.0	0.0	0.0	3.3332526785285337	0.0
22	0.0	0.0	0.0	3.5224057294367945	0.0
23	0.0	0.0	0.0	3.743750567652023	0.0
24	0.0	0.0	0.0	3.905516052234857	0.0
25	0.0	0.0	0.0	4.027971086304499	0.0
26	0.0	0.0	0.0	4.159929360417918	0.0
27	0.0	0.0	0.0	4.284628702099375	0.0
28	0.0	0.0	0.0	4.414167332068806	0.0
29	0.0	0.0	0.0	4.553384538614189	0.0
30	0.0	0.0	0.0	4.726055955498185	0.0
31	0.0	0.0	0.0	4.8940283533199445	0.0
32	0.0	0.0	0.0	5.053163789920183	0.0
33	0.0	0.0	0.0	5.200762082703437	0.0
34	0.0	0.0	0.0	5.354251682967704	0.0
35	3.506730643460526E-5	0.0	0.0	5.545894512632822	0.0
36	3.506730643460526E-5	0.0	0.0	5.716567093050045	0.0
37	3.506730643460526E-5	0.0	0.0	5.885170702387628	0.0
38	3.506730643460526E-5	0.0	0.0	6.053423638660863	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCG	20	7.858445E-4	44.000004	28
GATACGA	30	2.529041E-6	44.000004	35
CTCGTCG	20	7.858445E-4	44.000004	30
CGTTACA	30	2.529041E-6	44.000004	25
TACGCAC	20	7.858445E-4	44.000004	14
TCGCTAA	20	7.858445E-4	44.000004	23
TCGACGA	20	7.858445E-4	44.000004	14
GACTCGA	20	7.858445E-4	44.000004	34
TCACGAA	35	1.447097E-7	44.0	1
CCGGACT	25	4.4445045E-5	44.0	38
ATCGCTT	35	1.447097E-7	44.0	43
ATCGCTA	55	1.8189894E-12	44.0	1
TACCGGT	50	2.7284841E-11	44.0	41
CGTTTTA	8665	0.0	43.644547	1
ACATACG	785	0.0	42.878986	17
CGTTATT	4665	0.0	42.39657	1
AGGCGAT	1890	0.0	41.78836	7
CTAACGG	165	0.0	41.333332	2
GTTTTAT	9795	0.0	41.214905	2
TATTACG	70	0.0	40.857143	1
>>END_MODULE