FastQCFastQC Report
Thu 26 May 2016
SRR1546404_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546404_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1103946
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36730.3327155494924571No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31590.2861553010745091No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24690.22365224385975402No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG207.033167E-445.043
AGCCGAA253.8907056E-545.037
GGTACGA207.033167E-445.08
ACCGAAG207.033167E-445.02
TCGGAAC207.033167E-445.013
TACGCCC207.033167E-445.043
TCTACGA207.033167E-445.027
TCTCGTC207.033167E-445.028
CGCTAAG207.033167E-445.02
ACTCGAA207.033167E-445.01
GCGGCAT207.033167E-445.024
ATGCGAA502.1827873E-1145.01
GCGTGAC207.033167E-445.038
TAAGCGA207.033167E-445.02
GGTTACG207.033167E-445.08
CCGTTAG207.033167E-445.041
CTCGTAG302.1654232E-644.9999962
GCGAAAA302.1654232E-644.9999961
CGGTTTA650.044.9999961
TACGCAA600.044.9999961