FastQCFastQC Report
Thu 26 May 2016
SRR1546403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546403_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1245141
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39660.3185181437283007No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33120.2659939717670529No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16830.13516541500119264No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15890.12761606918413257No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14240.11436455790950584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCG207.856734E-444.00000426
TACCCGC207.856734E-444.00000419
CGTATTA302.527886E-644.00000428
CATTGCG207.856734E-444.00000414
GGCGTAA408.312782E-944.0000041
GAATCGT207.856734E-444.00000414
CACGATC207.856734E-444.00000415
CGCGATC207.856734E-444.00000436
CGATTCG207.856734E-444.00000431
TAGCCGA207.856734E-444.00000435
ATCGTAC302.527886E-644.00000428
ATCCGAT207.856734E-444.00000412
TAACCGT408.312782E-944.00000413
AACGCGC302.527886E-644.00000441
TATTACG302.527886E-644.0000041
CGGTCTA650.044.00000431
GATTCGC207.856734E-444.00000432
TTACCGC302.527886E-644.00000443
CGATCCA207.856734E-444.00000433
TTCGATC207.856734E-444.00000415
Produced by FastQC (version 0.11.5)