##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546403_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1245141
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.557079880913086	31.0	31.0	33.0	30.0	34.0
2	31.75400777903868	31.0	31.0	34.0	30.0	34.0
3	31.803521850135848	31.0	31.0	34.0	30.0	34.0
4	35.69172166043845	37.0	35.0	37.0	35.0	37.0
5	27.292146833169898	35.0	27.0	37.0	0.0	37.0
6	31.439426538841786	35.0	28.0	37.0	17.0	37.0
7	14.769080770772145	0.0	0.0	32.0	0.0	37.0
8	24.98342436720018	17.0	17.0	35.0	17.0	37.0
9	33.189984909339586	32.0	32.0	37.0	28.0	39.0
10	36.10173546610384	37.0	35.0	39.0	32.0	39.0
11	37.073174845258485	39.0	37.0	39.0	34.0	39.0
12	36.92623646639216	39.0	35.0	39.0	33.0	39.0
13	36.36803060858168	39.0	35.0	39.0	32.0	39.0
14	37.673952588502026	40.0	36.0	41.0	33.0	41.0
15	37.98421383602339	40.0	36.0	41.0	33.0	41.0
16	38.08132974498471	40.0	36.0	41.0	33.0	41.0
17	38.12115896914486	40.0	36.0	41.0	34.0	41.0
18	38.0899801709204	40.0	36.0	41.0	34.0	41.0
19	38.02357885572798	40.0	36.0	41.0	34.0	41.0
20	38.02691422095972	40.0	36.0	41.0	34.0	41.0
21	37.86989585918382	40.0	35.0	41.0	34.0	41.0
22	37.79515733559492	40.0	35.0	41.0	33.0	41.0
23	37.702656165044765	39.0	35.0	41.0	33.0	41.0
24	37.56874040771286	39.0	35.0	41.0	33.0	41.0
25	37.3816523590501	39.0	35.0	41.0	33.0	41.0
26	37.139017187611685	39.0	35.0	40.0	33.0	41.0
27	36.92029015187838	39.0	35.0	40.0	32.0	41.0
28	36.977608961555354	39.0	35.0	40.0	33.0	41.0
29	36.99944343652646	38.0	35.0	40.0	33.0	41.0
30	36.81471576311438	38.0	35.0	40.0	32.0	41.0
31	36.61911783484762	38.0	35.0	40.0	32.0	41.0
32	36.35121564545702	38.0	35.0	40.0	31.0	41.0
33	36.136095430156104	38.0	35.0	40.0	31.0	41.0
34	35.93700392164421	38.0	35.0	40.0	30.0	41.0
35	35.785311864278825	38.0	35.0	40.0	30.0	41.0
36	35.58189474123814	38.0	35.0	40.0	29.0	41.0
37	35.3747077640203	38.0	34.0	40.0	29.0	41.0
38	35.24398682558843	38.0	34.0	40.0	28.0	41.0
39	35.19072137211769	38.0	34.0	40.0	28.0	41.0
40	34.94648557874169	37.0	34.0	40.0	27.0	41.0
41	34.82178805452555	37.0	34.0	40.0	26.0	40.0
42	34.83302132047696	37.0	34.0	40.0	26.0	41.0
43	34.70773269854579	37.0	34.0	40.0	26.0	41.0
44	34.46620824468875	37.0	34.0	40.0	25.0	40.0
45	34.25585295159343	36.0	33.0	40.0	24.0	40.0
46	34.2409317498982	36.0	33.0	39.0	24.0	40.0
47	34.09815273932832	36.0	33.0	39.0	24.0	40.0
48	34.069568024826104	36.0	33.0	39.0	24.0	40.0
49	33.94822433764529	36.0	33.0	39.0	24.0	40.0
50	34.0372375498036	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	0.0
13	3.0
14	8.0
15	27.0
16	61.0
17	135.0
18	364.0
19	869.0
20	1564.0
21	2637.0
22	4358.0
23	6749.0
24	10275.0
25	15402.0
26	19422.0
27	21049.0
28	21764.0
29	24623.0
30	30068.0
31	40903.0
32	57941.0
33	113284.0
34	109154.0
35	128579.0
36	158992.0
37	305988.0
38	139242.0
39	31677.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.381250798102382	24.474497265771507	29.835416229969137	16.308835706156973
2	30.30291348530006	25.34451921509291	32.03034837018458	12.322218929422451
3	27.812914360702923	23.06823082687021	35.82405526763635	13.294799544790509
4	26.646701056346227	26.41732944301087	32.78560420064876	14.150365299994135
5	17.474406512997323	47.480968018883004	24.16465283851387	10.879972629605804
6	22.56780557382658	38.651365588314896	28.72020116597237	10.060627671886156
7	38.187803630271596	56.804972288279	3.9494322329760245	1.0577918484733857
8	87.7384970858722	3.2248556589173436	7.472727988235871	1.5639192669745834
9	83.38388985665077	5.344133716583102	8.980348410340676	2.2916280164254488
10	54.91586896584403	22.259808326928436	13.671142464989908	9.153180242237626
11	50.33839541064024	17.338518288290242	20.150488980766035	12.172597320303485
12	45.22427580490884	20.849767215118607	22.559212169545457	11.366744810427091
13	20.960437412309126	43.15575505103438	22.27097172127494	13.612835815381551
14	14.354518885812931	43.575225617018475	27.45054576148404	14.619709735684552
15	13.66463717763691	22.299723485131402	50.26916630325401	13.766473033977677
16	14.265292043230446	16.253741544130342	49.37954817968407	20.101418232955144
17	15.583295385823776	17.53825470368416	30.149356578893478	36.72909333159859
18	21.006135048159205	22.992737368699608	34.274270946021375	21.726856637119813
19	29.25564253365683	23.89335826223697	25.561763687807243	21.28923551629896
20	31.644528611619087	22.223346592875824	25.61573347918027	20.516391316324817
21	21.674332465158564	27.114278623866696	28.384415901492282	22.826973009482458
22	22.05790348241685	25.577183628199535	26.125474946210915	26.2394379431727
23	19.981190885208985	29.302143291402338	25.69275286895219	25.023912954436483
24	19.9845639971698	22.53664444428382	40.15360509372031	17.32518646482607
25	16.63707162482	25.1334587809734	37.04311399271247	21.186355601494128
26	16.220733234228092	34.39192830370215	28.43228196646002	20.955056495609732
27	17.475450571461383	33.53387287062268	29.929060242976497	19.061616314939435
28	14.799368103692675	29.301581106075535	39.63655521744124	16.26249557279055
29	14.87951966885678	23.83175881285734	39.15323646077031	22.135485057515574
30	17.703135628816334	30.12213074663833	32.667946842967986	19.506786781577347
31	29.348884985716477	26.160812309609916	24.900553431298142	19.589749273375464
32	31.13904369063423	25.860765969476553	26.140894886603206	16.859295453286013
33	27.51262708400093	28.02244886322111	24.860317024337004	19.604607028440956
34	19.298216025333677	26.99766532464998	30.112894844840866	23.591223805175478
35	21.49435284839227	24.12313143652004	30.771695735663673	23.610819979424015
36	29.4984262826459	24.983917484043978	26.395082966507406	19.122573266802714
37	20.403311753448	33.02445265235022	29.15477042359058	17.417465170611198
38	20.603128481031465	32.6146195491113	24.148751024984318	22.63350094487291
39	21.450582705091232	32.29891233201702	26.302402699774564	19.94810226311719
40	24.24946251067148	26.649833231738413	25.28693537519044	23.813768882399664
41	17.859583774046474	25.050737225743912	26.98738536438845	30.102293635821166
42	22.172268040326358	26.255420068891794	24.22408385877583	27.348228032006013
43	23.426824753180565	27.053080735434783	25.474544649963338	24.045549861421318
44	20.911768225445954	30.99632892981598	25.94204190529426	22.149860939443805
45	17.349681682636746	37.43792871650681	22.57061650046059	22.64177310039586
46	21.54117485489595	33.26249798215623	25.874740290457066	19.321586872490744
47	22.872590333142995	26.655936958143695	28.067022128417584	22.404450580295727
48	23.532033721482147	22.528211664381782	31.08467233831349	22.85508227582258
49	19.853092942887592	24.53625733953022	31.88016457573881	23.730485141843374
50	18.114173414898392	33.61418506016587	27.274421129815817	20.99722039511991
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	343.0
1	862.5
2	1382.0
3	7026.0
4	12670.0
5	9753.5
6	6837.0
7	7140.5
8	7444.0
9	7665.0
10	7886.0
11	7956.0
12	8026.0
13	7621.5
14	7217.0
15	6727.5
16	6238.0
17	5787.5
18	5337.0
19	5152.0
20	4967.0
21	4984.0
22	5001.0
23	5133.0
24	5265.0
25	7284.0
26	9303.0
27	11822.5
28	14342.0
29	17609.5
30	20877.0
31	21970.5
32	23064.0
33	25448.0
34	27832.0
35	29545.0
36	31258.0
37	38795.5
38	46333.0
39	66226.5
40	86120.0
41	105414.0
42	124708.0
43	128361.0
44	132014.0
45	126370.0
46	120726.0
47	112198.5
48	103671.0
49	95398.5
50	87126.0
51	80024.5
52	72923.0
53	66356.5
54	59790.0
55	54166.0
56	48542.0
57	46391.5
58	44241.0
59	39809.0
60	35377.0
61	30703.5
62	26030.0
63	22213.5
64	18397.0
65	15313.5
66	12230.0
67	10257.0
68	8284.0
69	6832.0
70	5380.0
71	4522.5
72	3665.0
73	2985.0
74	2305.0
75	1741.0
76	1177.0
77	827.0
78	477.0
79	300.5
80	124.0
81	107.5
82	91.0
83	79.5
84	68.0
85	46.5
86	25.0
87	22.0
88	19.0
89	11.0
90	3.0
91	3.5
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1245141.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.07810880846474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.70230079985924	13.68554430910983
2	5.879525457471043	2.1258140192459987
3	2.1529135663316135	1.1676181712208833
4	1.3056835409376009	0.9441715648996589
5	0.9590683498858159	0.8669070991995256
6	0.7533214479677501	0.8171176262466717
7	0.6480366253733282	0.820069363775853
8	0.5807398237976745	0.83989421792184
9	0.5463619467264069	0.8889471649552139
>10	8.819487883266843	38.569830702885525
>50	2.2525052739124267	28.579218575704434
>100	0.39312592042055605	9.075128706208073
>500	0.004157618429829618	0.5271353068878809
>1k	0.0027717456198864117	1.0926031717386162
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3966	0.3185181437283007	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3312	0.2659939717670529	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1683	0.13516541500119264	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1589	0.12761606918413257	No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1424	0.11436455790950584	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010279960261528614	0.0
2	0.0	0.0	0.0	0.033731119608140764	0.0
3	0.0	0.0	0.0	0.04770544058865622	0.0
4	0.0	0.0	0.0	0.07051410241892284	0.0
5	0.0	0.0	0.0	0.11524799199448095	0.0
6	0.0	0.0	0.0	0.18166617274670097	0.0
7	0.0	0.0	0.0	0.22648037451180228	0.0
8	0.0	0.0	8.03121895431923E-5	0.46902318693224304	0.0
9	0.0	0.0	8.03121895431923E-5	0.5733487211488498	0.0
10	8.03121895431923E-5	0.0	8.03121895431923E-5	0.7648129810198202	0.0
11	8.03121895431923E-5	0.0	8.03121895431923E-5	0.9610959722633822	0.0
12	8.03121895431923E-5	0.0	8.03121895431923E-5	1.130554692199518	0.0
13	8.03121895431923E-5	0.0	8.03121895431923E-5	1.19777599484717	0.0
14	8.03121895431923E-5	0.0	8.03121895431923E-5	1.2201027835401774	0.0
15	8.03121895431923E-5	0.0	8.03121895431923E-5	1.2614635611549214	0.0
16	8.03121895431923E-5	0.0	8.03121895431923E-5	1.3601672421035047	0.0
17	8.03121895431923E-5	0.0	8.03121895431923E-5	1.482241770209157	0.0
18	8.03121895431923E-5	0.0	8.03121895431923E-5	1.6533870461256999	0.0
19	8.03121895431923E-5	0.0	8.03121895431923E-5	1.729844250570819	0.0
20	8.03121895431923E-5	0.0	8.03121895431923E-5	1.804293650277358	0.0
21	8.03121895431923E-5	0.0	8.03121895431923E-5	1.9168110278273705	0.0
22	8.03121895431923E-5	0.0	8.03121895431923E-5	2.0302118394623583	0.0
23	8.03121895431923E-5	0.0	8.03121895431923E-5	2.1598357133850703	0.0
24	1.606243790863846E-4	0.0	8.03121895431923E-5	2.2550859701832966	0.0
25	1.606243790863846E-4	0.0	8.03121895431923E-5	2.330820364922527	0.0
26	1.606243790863846E-4	0.0	8.03121895431923E-5	2.4050288280604364	0.0
27	1.606243790863846E-4	0.0	8.03121895431923E-5	2.4714470088126568	0.0
28	1.606243790863846E-4	0.0	8.03121895431923E-5	2.546458593845998	0.0
29	1.606243790863846E-4	0.0	8.03121895431923E-5	2.6226748617224875	0.0
30	1.606243790863846E-4	0.0	8.03121895431923E-5	2.728446015350872	0.0
31	1.606243790863846E-4	0.0	8.03121895431923E-5	2.823776584338641	0.0
32	1.606243790863846E-4	0.0	8.03121895431923E-5	2.917259972966917	0.0
33	1.606243790863846E-4	0.0	8.03121895431923E-5	3.001507459797726	0.0
34	1.606243790863846E-4	0.0	8.03121895431923E-5	3.097801775060013	0.0
35	1.606243790863846E-4	0.0	8.03121895431923E-5	3.221803795714702	0.0
36	1.606243790863846E-4	0.0	8.03121895431923E-5	3.324442773950902	0.0
37	1.606243790863846E-4	0.0	8.03121895431923E-5	3.4240298889844603	0.0
38	1.606243790863846E-4	0.0	8.03121895431923E-5	3.5150235997368973	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCG	20	7.856734E-4	44.000004	26
TACCCGC	20	7.856734E-4	44.000004	19
CGTATTA	30	2.527886E-6	44.000004	28
CATTGCG	20	7.856734E-4	44.000004	14
GGCGTAA	40	8.312782E-9	44.000004	1
GAATCGT	20	7.856734E-4	44.000004	14
CACGATC	20	7.856734E-4	44.000004	15
CGCGATC	20	7.856734E-4	44.000004	36
CGATTCG	20	7.856734E-4	44.000004	31
TAGCCGA	20	7.856734E-4	44.000004	35
ATCGTAC	30	2.527886E-6	44.000004	28
ATCCGAT	20	7.856734E-4	44.000004	12
TAACCGT	40	8.312782E-9	44.000004	13
AACGCGC	30	2.527886E-6	44.000004	41
TATTACG	30	2.527886E-6	44.000004	1
CGGTCTA	65	0.0	44.000004	31
GATTCGC	20	7.856734E-4	44.000004	32
TTACCGC	30	2.527886E-6	44.000004	43
CGATCCA	20	7.856734E-4	44.000004	33
TTCGATC	20	7.856734E-4	44.000004	15
>>END_MODULE