FastQCFastQC Report
Thu 26 May 2016
SRR1546402_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546402_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences477205
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12820.26864764618979264No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10960.22967068660219403No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.19404658375331357No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5830.122169717417043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGA551.8189894E-1245.0000044
CTAGTAA650.045.0000041
GTTATCC302.1628239E-645.00000424
TCCGGAA207.0291356E-445.019
TCGCCCG207.0291356E-445.026
AACGTAA207.0291356E-445.01
GGCAGTA253.887366E-545.08
GTCGCCC207.0291356E-445.025
CTATCCA207.0291356E-445.031
TAGGTTA207.0291356E-445.06
GGACTAT406.8012014E-945.08
CCGTGTT351.2099372E-745.024
CGACCCG253.887366E-545.032
ACTTCGA207.0291356E-445.038
GATCAGT207.0291356E-445.09
CGCGATC207.0291356E-445.036
GCGTTCC253.887366E-545.011
TCGATCA253.887366E-545.017
CGTTCGT207.0291356E-445.040
CGTTCAC253.887366E-545.022