##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546402_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	477205
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.032306870213013	31.0	31.0	33.0	30.0	33.0
2	31.184687922381368	31.0	31.0	33.0	30.0	34.0
3	31.148332477656353	31.0	31.0	34.0	28.0	34.0
4	35.106151444347816	35.0	35.0	37.0	33.0	37.0
5	32.828539097452875	35.0	35.0	37.0	28.0	37.0
6	34.00960803009189	35.0	35.0	37.0	28.0	37.0
7	14.898165358703283	0.0	0.0	35.0	0.0	37.0
8	24.707961986986724	17.0	17.0	35.0	17.0	37.0
9	32.6469211345229	32.0	32.0	37.0	27.0	39.0
10	35.53781708070955	37.0	35.0	37.0	32.0	39.0
11	36.49354470301024	37.0	35.0	39.0	32.0	39.0
12	36.254888360348275	37.0	35.0	39.0	32.0	39.0
13	35.79906539118408	37.0	35.0	39.0	31.0	39.0
14	36.82835469033225	39.0	35.0	40.0	31.0	41.0
15	37.25428694167077	39.0	35.0	41.0	32.0	41.0
16	37.354690332247145	39.0	35.0	41.0	32.0	41.0
17	37.36220073134188	39.0	35.0	41.0	32.0	41.0
18	37.237922905250365	39.0	35.0	41.0	32.0	41.0
19	37.19110864303601	39.0	35.0	40.0	32.0	41.0
20	37.270370176339306	39.0	35.0	41.0	32.0	41.0
21	37.09409792437213	39.0	35.0	40.0	32.0	41.0
22	37.14413092905565	39.0	35.0	41.0	32.0	41.0
23	37.144097400488256	39.0	35.0	41.0	32.0	41.0
24	36.977948680336546	39.0	35.0	40.0	32.0	41.0
25	36.708814869919635	38.0	35.0	40.0	31.0	41.0
26	36.55917896920611	38.0	35.0	40.0	31.0	41.0
27	36.46976666212634	38.0	35.0	40.0	31.0	41.0
28	36.426405842352864	38.0	35.0	40.0	31.0	41.0
29	36.50707347995097	38.0	35.0	40.0	31.0	41.0
30	36.26505170733752	38.0	35.0	40.0	30.0	41.0
31	36.135168323885964	38.0	35.0	40.0	30.0	41.0
32	35.993413732043884	38.0	34.0	40.0	30.0	41.0
33	35.775920202009615	38.0	34.0	40.0	29.0	41.0
34	35.63164468100712	38.0	34.0	40.0	29.0	41.0
35	35.45462013180918	38.0	34.0	40.0	27.0	41.0
36	35.2232415838057	38.0	34.0	40.0	26.0	41.0
37	35.035749834976585	38.0	34.0	40.0	26.0	41.0
38	35.01594073825714	38.0	34.0	40.0	26.0	41.0
39	34.92624553389005	38.0	34.0	40.0	25.0	41.0
40	34.743638478222145	37.0	34.0	40.0	24.0	41.0
41	34.67849037625339	37.0	34.0	40.0	24.0	41.0
42	34.622824572248824	37.0	33.0	40.0	24.0	41.0
43	34.544114164771955	37.0	33.0	40.0	23.0	41.0
44	34.30299766347796	37.0	33.0	40.0	23.0	41.0
45	34.205282844898946	37.0	33.0	40.0	23.0	41.0
46	34.201227983780555	37.0	33.0	40.0	23.0	41.0
47	34.17345166123574	37.0	33.0	40.0	23.0	41.0
48	34.16928364120242	37.0	33.0	40.0	23.0	41.0
49	34.083192757829444	36.0	33.0	40.0	23.0	41.0
50	33.982032878951394	36.0	33.0	40.0	23.0	41.0
51	33.802667616642744	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	1.0
13	5.0
14	7.0
15	15.0
16	38.0
17	148.0
18	299.0
19	611.0
20	961.0
21	1608.0
22	2423.0
23	3643.0
24	5142.0
25	6948.0
26	8440.0
27	9019.0
28	9556.0
29	11238.0
30	13713.0
31	17961.0
32	24085.0
33	37438.0
34	48041.0
35	47804.0
36	53498.0
37	83029.0
38	77628.0
39	13903.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.915036514705424	25.403757295082823	29.960080049454636	15.721126140757116
2	29.763937930239624	25.148311522301736	32.726396412443286	12.36135413501535
3	28.18893347722677	22.711832440984484	35.69157909074716	13.407654991041587
4	27.19460190065066	26.926582915099377	31.42905040810553	14.449764776144425
5	21.172452090820506	36.24815330937437	29.20547772969688	13.373916870108236
6	22.578346832074267	39.36337632673589	28.14995651763917	9.908320323550674
7	36.76072128330592	58.285223331691824	4.183526995735585	0.7705283892666673
8	88.22099516979077	3.4727213671273347	6.803574983497658	1.5027084795842458
9	83.75121802998711	5.549187456124726	8.317808908121247	2.3817856057669133
10	55.320669314026475	22.630944772162906	13.038840749782587	9.009545164028038
11	50.902442346580614	17.616328412317557	19.663457004851164	11.817772236250669
12	45.77718171435756	21.050701480495803	21.932293249232508	11.239823555914125
13	20.968137383304867	44.12946218082375	21.43607045190222	13.466329983969153
14	14.174620970023366	44.421370270638405	26.85240095975524	14.551607799582989
15	13.662681656730335	22.474617826720173	50.18115904066387	13.681541475885625
16	14.177554719669743	16.634150941419307	49.083308012279836	20.104986326631114
17	15.507591077210003	17.880784987583954	29.62416571494431	36.98745822026173
18	21.07207594220513	23.330015402185644	34.09708615794051	21.500822497668718
19	30.054798252323423	23.816598736392116	25.08376903008141	21.04483398120305
20	32.37162225877767	22.323948827024026	25.080206619796524	20.224222294401777
21	21.684600957659704	27.847780304062198	27.783447365388042	22.68417137289006
22	21.828354690332247	25.95844553179451	25.463689609287414	26.74951016858583
23	19.973805806728766	29.73669596923754	25.181630536142745	25.107867687890952
24	19.65402709527352	22.792510556259888	40.49077440512987	17.062687943336723
25	16.439685250573653	25.475424607872927	37.18632453557695	20.89856560597647
26	15.919992456072338	35.342043775735796	28.002011714043228	20.735952054148637
27	17.312266216825055	34.773105897884555	29.273792185748267	18.640835699542123
28	14.561875923345314	30.02462254167496	39.396485787030734	16.017015747948996
29	14.603786632579292	24.37191563374231	38.99204744292285	22.032250290755545
30	17.66075376410557	30.99255037143366	32.097945327479806	19.248750536980964
31	30.01477352500498	26.557349566748044	24.396433398644188	19.03144350960279
32	32.01621944447355	25.876719648788253	25.740509843777833	16.36655106296036
33	28.2798797162645	28.267096949948133	23.967896396726772	19.485126937060592
34	19.374063557590553	27.21304261271361	29.47580180425603	23.937092025439803
35	21.519891870370174	24.097819595352103	30.804161733426934	23.57812680085079
36	30.004505401242653	25.38070640500414	26.021521149191646	18.59326704456156
37	20.132228287633197	34.16519106044572	28.801039385589	16.901541266332078
38	20.259008183065976	33.67567397659287	23.765258117580494	22.30005972276066
39	21.608323466853868	33.071950210077425	25.93937615909305	19.38035016397565
40	24.640144172839765	26.80210810867447	24.935824226485472	23.621923492000292
41	17.72278161377186	25.05296465879444	26.731069456522878	30.493184270910824
42	21.92914994603996	25.901866074328645	24.170325122326883	27.998658857304516
43	23.229220146477928	26.75013882922434	25.429951488354064	24.59068953594367
44	20.449492356534403	31.08286794983288	26.565731708594836	21.901907985037877
45	17.26344024056747	38.37889376682977	22.243480265294792	22.11418572730797
46	21.28225814901353	34.277092654100436	25.276767846103876	19.16388135078216
47	22.48299996856697	27.018367368321787	27.697111304366047	22.801521358745195
48	23.21182720214583	22.88115170628975	31.51643423685837	22.390586854706047
49	19.87447742584424	25.03536216091617	32.05561551115349	23.034544902086104
50	18.062258358567075	34.72323215389612	26.81342400016764	20.401085487369162
51	18.63224400414916	34.410997370103	23.67976027074318	23.276998355004665
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	137.0
1	346.0
2	555.0
3	2306.5
4	4058.0
5	3150.0
6	2242.0
7	2349.5
8	2457.0
9	2582.0
10	2707.0
11	2629.0
12	2551.0
13	2498.5
14	2446.0
15	2338.0
16	2230.0
17	2080.5
18	1931.0
19	1883.0
20	1835.0
21	1877.5
22	1920.0
23	1855.5
24	1791.0
25	2362.0
26	3912.0
27	4891.0
28	6021.0
29	7151.0
30	8048.0
31	8945.0
32	10162.0
33	11379.0
34	11822.0
35	12265.0
36	14098.5
37	15932.0
38	22373.0
39	28814.0
40	36235.0
41	43656.0
42	46927.5
43	50199.0
44	47513.5
45	44828.0
46	42850.5
47	40873.0
48	38065.0
49	35257.0
50	32604.5
51	29952.0
52	26986.5
53	24021.0
54	22487.5
55	20954.0
56	19249.0
57	17544.0
58	16202.5
59	14861.0
60	13197.5
61	11534.0
62	9813.0
63	8092.0
64	6801.0
65	5510.0
66	4644.0
67	3778.0
68	3188.5
69	2599.0
70	2215.5
71	1832.0
72	1483.5
73	1135.0
74	932.0
75	558.5
76	388.0
77	281.0
78	174.0
79	113.5
80	53.0
81	38.0
82	23.0
83	23.0
84	23.0
85	16.0
86	9.0
87	9.0
88	9.0
89	5.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	477205.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.218322566564826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.68091022345047	18.076723158799485
2	6.071297702599603	3.062158877236015
3	3.1284427332929936	2.3668223393762537
4	2.1457283548919914	2.164466791755629
5	1.6439931099869898	2.0729374272431
6	1.3150253290428355	1.9897639758603167
7	1.1371184220617234	2.0073353414755024
8	0.9610009747596232	1.9387866054617124
9	0.8937169979023201	2.028423918269039
>10	10.688038204685668	57.45996451533741
>50	0.31064082456538583	4.913326122708315
>100	0.020764761000360017	1.1051161113274959
>500	0.0016611808800288013	0.3160766133421515
>1k	0.0016611808800288013	0.4980982018075787
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1282	0.26864764618979264	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1096	0.22967068660219403	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	926	0.19404658375331357	No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	583	0.122169717417043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009429909577644828	0.0
2	0.0	0.0	0.0	0.03269035320250207	0.0
3	0.0	0.0	0.0	0.04253936987248667	0.0
4	0.0	0.0	0.0	0.06328517094330528	0.0
5	0.0	0.0	0.0	0.10310034471558345	0.0
6	0.0	0.0	0.0	0.14207730430318208	0.0
7	0.0	0.0	0.0	0.1682714975744177	0.0
8	0.0	0.0	0.0	0.37007156253601703	0.0
9	0.0	0.0	0.0	0.4499114636267432	0.0
10	0.0	0.0	0.0	0.5945034104839639	0.0
11	0.0	0.0	0.0	0.7464297314571305	0.0
12	0.0	0.0	0.0	0.8838968577445752	0.0
13	0.0	0.0	0.0	0.9436196184029925	0.0
14	0.0	0.0	0.0	0.960803009188923	0.0
15	0.0	0.0	0.0	0.9932838088452551	0.0
16	0.0	0.0	0.0	1.068303978374074	0.0
17	0.0	0.0	0.0	1.1655368237969006	0.0
18	0.0	0.0	0.0	1.3059376997307237	0.0
19	0.0	0.0	0.0	1.3679655493970098	0.0
20	0.0	0.0	0.0	1.432717595163504	0.0
21	0.0	0.0	0.0	1.5270166909399525	0.0
22	0.0	0.0	0.0	1.6271832860091575	0.0
23	0.0	0.0	0.0	1.742856843494934	0.0
24	0.0	0.0	0.0	1.8252113871396989	0.0
25	0.0	0.0	0.0	1.8945736109219309	0.0
26	0.0	0.0	0.0	1.9532486038494987	0.0
27	0.0	0.0	0.0	2.018419756708333	0.0
28	0.0	0.0	0.0	2.086105552121206	0.0
29	0.0	0.0	0.0	2.1571442042727966	0.0
30	0.0	0.0	0.0	2.253329281964774	0.0
31	0.0	0.0	0.0	2.342808646179315	0.0
32	0.0	0.0	0.0	2.417828815708134	0.0
33	0.0	0.0	0.0	2.4894961284982347	0.0
34	0.0	0.0	0.0	2.5814901352668143	0.0
35	0.0	0.0	0.0	2.6902484257289845	0.0
36	0.0	0.0	0.0	2.7797277899435255	0.0
37	0.0	0.0	0.0	2.8650160832346687	0.0
38	0.0	0.0	0.0	2.9459037520562443	0.0
39	0.0	0.0	0.0	3.037688205278654	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGGA	55	1.8189894E-12	45.000004	4
CTAGTAA	65	0.0	45.000004	1
GTTATCC	30	2.1628239E-6	45.000004	24
TCCGGAA	20	7.0291356E-4	45.0	19
TCGCCCG	20	7.0291356E-4	45.0	26
AACGTAA	20	7.0291356E-4	45.0	1
GGCAGTA	25	3.887366E-5	45.0	8
GTCGCCC	20	7.0291356E-4	45.0	25
CTATCCA	20	7.0291356E-4	45.0	31
TAGGTTA	20	7.0291356E-4	45.0	6
GGACTAT	40	6.8012014E-9	45.0	8
CCGTGTT	35	1.2099372E-7	45.0	24
CGACCCG	25	3.887366E-5	45.0	32
ACTTCGA	20	7.0291356E-4	45.0	38
GATCAGT	20	7.0291356E-4	45.0	9
CGCGATC	20	7.0291356E-4	45.0	36
GCGTTCC	25	3.887366E-5	45.0	11
TCGATCA	25	3.887366E-5	45.0	17
CGTTCGT	20	7.0291356E-4	45.0	40
CGTTCAC	25	3.887366E-5	45.0	22
>>END_MODULE