FastQCFastQC Report
Thu 26 May 2016
SRR1546401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546401_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2126506
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65440.307734847679715No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA38790.18241190008398753No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28910.1359507097558154No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA26730.12569915156599606No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25380.11935070956771343No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA23310.1096164318370134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG207.857993E-444.0000041
CGAAATC207.857993E-444.00000413
CGACGAA207.857993E-444.00000411
ATTACCG207.857993E-444.00000419
CCGTCTA207.857993E-444.00000418
CGCAATA207.857993E-444.00000415
TGTACCG207.857993E-444.00000422
CTTAACG351.4468606E-744.01
GACCGAC351.4468606E-744.09
TTATCGC351.4468606E-744.031
CGCGTAG454.802132E-1044.02
TGCCGAT302.5287354E-644.033
CGTTTTA40750.043.460121
AGGCGAT19600.042.5408177
CGTTATT25400.042.527561
TCGTTAG800.041.2500042
GTCGAAA650.040.6153871
TAGGCGA6550.040.3053446
TTACGTA557.8216544E-1140.00000418
ATAACGG1600.039.8750042
Produced by FastQC (version 0.11.5)