##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546401_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2126506 Sequences flagged as poor quality 0 Sequence length 50 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.57116321327097 31.0 31.0 33.0 30.0 34.0 2 31.771351691460076 31.0 31.0 34.0 30.0 34.0 3 31.846370525171338 31.0 31.0 34.0 30.0 34.0 4 35.73724927181019 37.0 35.0 37.0 35.0 37.0 5 27.25916879613789 35.0 26.0 37.0 0.0 37.0 6 31.4424370305092 36.0 28.0 37.0 17.0 37.0 7 14.822810281278304 0.0 0.0 32.0 0.0 37.0 8 24.993230209555016 17.0 17.0 35.0 17.0 37.0 9 33.09949325325205 32.0 32.0 37.0 28.0 39.0 10 36.06786108292194 37.0 35.0 39.0 32.0 39.0 11 37.0667809072723 39.0 37.0 39.0 34.0 39.0 12 37.07983471478566 39.0 35.0 39.0 34.0 39.0 13 36.60812760462468 39.0 35.0 39.0 33.0 39.0 14 37.95560887201823 40.0 36.0 41.0 33.0 41.0 15 38.21207417237478 40.0 37.0 41.0 33.0 41.0 16 38.269350286338245 40.0 37.0 41.0 34.0 41.0 17 38.29026017326074 40.0 36.0 41.0 34.0 41.0 18 38.2645362862837 40.0 36.0 41.0 34.0 41.0 19 38.20444804764247 40.0 36.0 41.0 34.0 41.0 20 38.24261723221096 40.0 36.0 41.0 34.0 41.0 21 38.11900131013033 40.0 36.0 41.0 34.0 41.0 22 38.038805439533206 40.0 36.0 41.0 34.0 41.0 23 37.94017181235322 40.0 36.0 41.0 34.0 41.0 24 37.82433202633804 40.0 35.0 41.0 34.0 41.0 25 37.6453675653866 40.0 35.0 41.0 33.0 41.0 26 37.38462012333847 39.0 35.0 41.0 33.0 41.0 27 37.18374131086392 39.0 35.0 40.0 33.0 41.0 28 37.21384139052512 39.0 35.0 40.0 33.0 41.0 29 37.20906595137752 39.0 35.0 40.0 33.0 41.0 30 37.04044192680387 39.0 35.0 40.0 32.0 41.0 31 36.885888400973236 39.0 35.0 40.0 32.0 41.0 32 36.67847304451527 38.0 35.0 40.0 32.0 41.0 33 36.47465796005278 38.0 35.0 40.0 31.0 41.0 34 36.28468059812669 38.0 35.0 40.0 31.0 41.0 35 36.1528761263782 38.0 35.0 40.0 30.0 41.0 36 35.968588849502424 38.0 35.0 40.0 30.0 41.0 37 35.824425607075646 38.0 35.0 40.0 30.0 41.0 38 35.682430240027536 38.0 35.0 40.0 30.0 41.0 39 35.60840552530771 38.0 35.0 40.0 29.0 41.0 40 35.37292770394252 38.0 34.0 40.0 29.0 41.0 41 35.21213765679476 38.0 34.0 40.0 28.0 41.0 42 35.22088863139817 38.0 34.0 40.0 28.0 41.0 43 35.06156577973446 38.0 34.0 40.0 27.0 41.0 44 34.86768342059698 37.0 34.0 40.0 27.0 40.0 45 34.64179503843394 37.0 34.0 40.0 26.0 40.0 46 34.58255184796093 37.0 34.0 40.0 26.0 40.0 47 34.46280518371451 37.0 33.0 39.0 26.0 40.0 48 34.39655237276546 36.0 33.0 39.0 26.0 40.0 49 34.237572336969656 36.0 33.0 39.0 26.0 40.0 50 34.362077511185014 36.0 34.0 39.0 26.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 3.0 12 10.0 13 7.0 14 20.0 15 33.0 16 105.0 17 224.0 18 579.0 19 1148.0 20 2239.0 21 3988.0 22 6374.0 23 9757.0 24 14883.0 25 21923.0 26 28795.0 27 31542.0 28 33678.0 29 38687.0 30 48345.0 31 65298.0 32 92873.0 33 171003.0 34 177544.0 35 221773.0 36 295725.0 37 550530.0 38 246960.0 39 62459.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.997088181270122 23.458104515106 29.227897781619237 15.31690952200464 2 31.199676840789543 25.286079606641128 30.2263431187121 13.287900433857228 3 29.15547851734253 23.882650695554116 33.46296695142173 13.49890383568163 4 27.52331759233221 27.233875662706808 30.85662584540086 14.386180899560125 5 18.029575275122667 47.43513538170125 23.14909997902663 11.386189364149454 6 22.43407730803487 40.085050312578474 26.821979340758972 10.658893038627683 7 38.33631318228117 56.62988959353983 3.7604408358123607 1.2733563883666448 8 85.94130465655869 4.047296363142169 7.869951930537698 2.1414470497614397 9 80.78857995227852 6.2953031874821885 9.391226735311351 3.5248901249279334 10 49.987961472951405 23.77707845639749 14.655872120746427 11.579087949904679 11 44.17852571307112 20.742805334196092 21.53353905420441 13.545129898528385 12 40.34044578289457 21.210426869239964 24.92280764785051 13.526319700014955 13 22.64926597902851 36.11370012593428 25.437078475207684 15.799955419829523 14 16.90416109806415 38.314446326509305 28.015204283458406 16.766188291968138 15 16.589231349453044 22.859516972912374 44.781016371456275 15.770235306178304 16 17.76538603700154 18.615654035304864 44.099899083284974 19.519060844408624 17 18.314690859089982 20.25510391223914 29.380918746525992 32.04928648214489 18 22.739696008381824 24.201483560356753 31.754013390980322 21.304807040281098 19 29.704172007979285 24.205998007999977 25.878977063784443 20.210852920236295 20 31.513854181460104 22.321686371917128 26.24845638808449 19.91600305853828 21 24.397815007340682 26.40420483177569 27.688141956806138 21.509838204077486 22 23.73513171371254 24.9912767704394 26.20213627424517 25.07145524160289 23 22.86370224208161 28.008620713978704 25.931457517636915 23.196219526302773 24 21.790298263912728 22.96612377298724 35.875892191228246 19.36768577187179 25 19.901613256675503 25.119703400789838 33.023842867125694 21.954840475408957 26 18.845091431672426 32.17004795660111 27.603354987006856 21.38150562471961 27 20.05599796097448 30.928951058685 29.395496650373897 19.619554329966622 28 17.3291775334751 27.982051308578487 36.45938454911484 18.229386608831575 29 18.68703873866333 24.473243903379537 35.435968673495395 21.40374868446174 30 20.56754130954721 27.987976521110213 30.426154452421013 21.01832771692156 31 28.025785020122214 25.952383863483103 25.95718046410403 20.06465065229066 32 28.725242251844108 26.87709322240332 26.536957807784223 17.86070671796835 33 26.879726650195202 27.970812214966713 25.766021821711295 19.38343931312679 34 21.37059570958182 27.77636178783413 28.773772564008755 22.079269938575298 35 22.894268814665935 25.406041647660526 29.637254726767758 22.062434810905778 36 27.97288133680319 26.70761333379732 26.04963258979754 19.269872739601958 37 22.247574189774213 31.300546530317806 28.018684170183388 18.43319510972459 38 22.021381552650215 31.098148794313303 24.94726090591797 21.933208747118513 39 23.187990064453146 29.117764069323105 27.246478495710804 20.447767370512945 40 24.18182690291022 27.071543649535908 25.590804822558695 23.15582462499518 41 18.111352613159802 26.26294024093983 28.04577085604273 27.57993628985764 42 23.2857560712267 26.494211631662456 24.90174022551547 25.318292071595376 43 24.005199138869113 27.250851866865176 25.279684139146564 23.46426485511915 44 21.769607045547957 30.274967481869318 26.643376505873956 21.312048966708772 45 19.729688042262755 33.68826610411633 23.208634257321634 23.37341159629928 46 21.26982007104612 32.26094824091726 25.829459216197836 20.639772471838782 47 23.642679588019032 27.198183311027574 27.454331189284204 21.70480591166919 48 23.691398002168814 23.725726614455827 29.58754877719602 22.99532660617934 49 20.54073677666557 25.557651847678777 30.702805447057287 23.198805928598368 50 19.574221751549256 31.367416786033054 27.234769147136195 21.8235923152815 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1037.0 1 2344.0 2 3651.0 3 12893.5 4 22136.0 5 16656.5 6 11177.0 7 11295.5 8 11414.0 9 11475.5 10 11537.0 11 11392.0 12 11247.0 13 10723.5 14 10200.0 15 9728.0 16 9256.0 17 9036.5 18 8817.0 19 8176.0 20 7535.0 21 8028.5 22 8522.0 23 10648.5 24 12775.0 25 16169.5 26 19564.0 27 23918.5 28 28273.0 29 32912.0 30 37551.0 31 43863.0 32 50175.0 33 53510.5 34 56846.0 35 59097.5 36 61349.0 37 68798.0 38 76247.0 39 97791.0 40 119335.0 41 147506.5 42 175678.0 43 181973.0 44 188268.0 45 180636.5 46 173005.0 47 165118.0 48 157231.0 49 153014.0 50 148797.0 51 139626.5 52 130456.0 53 119740.5 54 109025.0 55 102983.0 56 96941.0 57 96228.0 58 95515.0 59 91538.0 60 87561.0 61 76764.5 62 65968.0 63 55236.0 64 44504.0 65 35878.5 66 27253.0 67 23163.5 68 19074.0 69 15847.0 70 12620.0 71 10215.5 72 7811.0 73 5962.0 74 4113.0 75 3181.5 76 2250.0 77 1680.0 78 1110.0 79 761.5 80 413.0 81 295.5 82 178.0 83 118.0 84 58.0 85 39.0 86 20.0 87 12.5 88 5.0 89 5.0 90 5.0 91 4.0 92 3.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2126506.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.695232465861594 #Duplication Level Percentage of deduplicated Percentage of total 1 77.43943845246736 12.928694269897234 2 5.876057341025627 1.962042865823108 3 1.9865770751449852 0.9949909824269074 4 1.0209143920346546 0.6817761241104926 5 0.7426871735989348 0.6199667506323959 6 0.5749782182648374 0.5759637010043019 7 0.4919726515977419 0.5749518449689444 8 0.4330944539245099 0.578449007035606 9 0.3748210673916949 0.5631952367886048 >10 7.844757083360021 33.873028333764964 >50 2.510205090020184 29.65101867592917 >100 0.6983226190390166 15.258101751024514 >500 0.0027784719586955893 0.3241952330271012 >1k 0.0030871910652173215 1.076338481924476 >5k 3.0871910652173213E-4 0.337286741642186 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6544 0.307734847679715 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 3879 0.18241190008398753 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2891 0.1359507097558154 No Hit AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 2673 0.12569915156599606 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2538 0.11935070956771343 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 2331 0.1096164318370134 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007100849938819829 0.0 2 0.0 0.0 0.0 0.03249461793195035 0.0 3 0.0 0.0 0.0 0.04392181352885908 0.0 4 0.0 0.0 0.0 0.060474788220677485 0.0 5 0.0 0.0 0.0 0.09612011440362736 0.0 6 0.0 0.0 0.0 0.15396147483242464 0.0 7 0.0 0.0 0.0 0.19393314667346342 0.0 8 0.0 0.0 0.0 0.38575014601416596 0.0 9 0.0 0.0 0.0 0.4686560959621087 0.0 10 0.0 0.0 0.0 0.613024369552684 0.0 11 0.0 0.0 0.0 0.7730991589019734 0.0 12 0.0 0.0 0.0 0.9134232398121613 0.0 13 4.702549628357503E-5 0.0 0.0 0.9675966115308398 0.0 14 4.702549628357503E-5 0.0 0.0 0.9906861302060751 0.0 15 4.702549628357503E-5 0.0 0.0 1.0272249408184129 0.0 16 4.702549628357503E-5 0.0 0.0 1.1155858483352503 0.0 17 4.702549628357503E-5 0.0 0.0 1.218336557714862 0.0 18 4.702549628357503E-5 0.0 0.0 1.365056106119616 0.0 19 4.702549628357503E-5 0.0 0.0 1.4311739538943224 4.702549628357503E-5 20 4.702549628357503E-5 0.0 0.0 1.501947325801103 4.702549628357503E-5 21 4.702549628357503E-5 0.0 0.0 1.5999484600560732 4.702549628357503E-5 22 4.702549628357503E-5 0.0 0.0 1.7021348634802818 4.702549628357503E-5 23 4.702549628357503E-5 0.0 0.0 1.8131620602058023 4.702549628357503E-5 24 4.702549628357503E-5 0.0 0.0 1.893857811828417 4.702549628357503E-5 25 4.702549628357503E-5 0.0 0.0 1.9597875576179893 4.702549628357503E-5 26 4.702549628357503E-5 0.0 0.0 2.0315014394504414 4.702549628357503E-5 27 4.702549628357503E-5 0.0 0.0 2.0980895421879833 4.702549628357503E-5 28 4.702549628357503E-5 0.0 0.0 2.1724838773085993 4.702549628357503E-5 29 4.702549628357503E-5 0.0 0.0 2.249652716709946 4.702549628357503E-5 30 4.702549628357503E-5 0.0 0.0 2.344832321187902 4.702549628357503E-5 31 4.702549628357503E-5 0.0 0.0 2.4403881296361263 4.702549628357503E-5 32 4.702549628357503E-5 0.0 0.0 2.5257864308870985 4.702549628357503E-5 33 4.702549628357503E-5 0.0 0.0 2.610949604656653 4.702549628357503E-5 34 4.702549628357503E-5 0.0 0.0 2.703542806839012 4.702549628357503E-5 35 4.702549628357503E-5 0.0 0.0 2.8139116466165626 4.702549628357503E-5 36 4.702549628357503E-5 0.0 0.0 2.9160510245444877 4.702549628357503E-5 37 4.702549628357503E-5 0.0 0.0 3.0182844534649798 4.702549628357503E-5 38 4.702549628357503E-5 0.0 0.0 3.1179785055861586 4.702549628357503E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 20 7.857993E-4 44.000004 1 CGAAATC 20 7.857993E-4 44.000004 13 CGACGAA 20 7.857993E-4 44.000004 11 ATTACCG 20 7.857993E-4 44.000004 19 CCGTCTA 20 7.857993E-4 44.000004 18 CGCAATA 20 7.857993E-4 44.000004 15 TGTACCG 20 7.857993E-4 44.000004 22 CTTAACG 35 1.4468606E-7 44.0 1 GACCGAC 35 1.4468606E-7 44.0 9 TTATCGC 35 1.4468606E-7 44.0 31 CGCGTAG 45 4.802132E-10 44.0 2 TGCCGAT 30 2.5287354E-6 44.0 33 CGTTTTA 4075 0.0 43.46012 1 AGGCGAT 1960 0.0 42.540817 7 CGTTATT 2540 0.0 42.52756 1 TCGTTAG 80 0.0 41.250004 2 GTCGAAA 65 0.0 40.615387 1 TAGGCGA 655 0.0 40.305344 6 TTACGTA 55 7.8216544E-11 40.000004 18 ATAACGG 160 0.0 39.875004 2 >>END_MODULE