FastQCFastQC Report
Thu 26 May 2016
SRR1546399_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546399_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences719793
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31390.43609760028230343No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20540.2853598187256614No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.124758090173147No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7940.11030949175665783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACG302.5263926E-644.0000041
GAGTACG302.5263926E-644.0000041
TGACGTA302.5263926E-644.00000413
CGGATGG302.5263926E-644.0000042
CGAATTT302.5263926E-644.00000421
CGATGCG302.5263926E-644.00000410
ATCGCAA302.5263926E-644.0000041
CGGACTC302.5263926E-644.00000428
CTAGCGA302.5263926E-644.0000041
TCACGAC254.4411754E-544.025
TATCACG207.854519E-444.01
AAGTAAC207.854519E-444.042
ACTATCG254.4411754E-544.013
GATCGTA254.4411754E-544.021
CCGATTC254.4411754E-544.038
CGACGGT254.4411754E-544.028
CGCGGAC254.4411754E-544.026
GAGTCAC207.854519E-444.09
ACCGCAA207.854519E-444.028
TAGCGGC254.4411754E-544.043
Produced by FastQC (version 0.11.5)