##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546399_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	719793
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.579274874859856	31.0	31.0	33.0	30.0	34.0
2	31.755931219114384	31.0	31.0	34.0	30.0	34.0
3	31.802616863459356	31.0	31.0	34.0	30.0	34.0
4	35.69690591600641	37.0	35.0	37.0	35.0	37.0
5	26.998562086599897	35.0	19.0	37.0	0.0	37.0
6	31.289488783580836	35.0	25.0	37.0	17.0	37.0
7	15.16141585150175	0.0	0.0	32.0	0.0	37.0
8	25.204262892248188	17.0	17.0	35.0	17.0	37.0
9	33.33489628268127	32.0	32.0	37.0	28.0	39.0
10	36.21269448299719	37.0	35.0	39.0	33.0	39.0
11	37.16055171417338	39.0	37.0	39.0	34.0	39.0
12	36.80934518674119	39.0	35.0	39.0	33.0	39.0
13	36.08401443192696	38.0	35.0	39.0	32.0	39.0
14	37.350006182332976	39.0	35.0	41.0	33.0	41.0
15	37.75382644732583	40.0	35.0	41.0	33.0	41.0
16	37.8985180461605	40.0	35.0	41.0	33.0	41.0
17	37.96714472077389	40.0	35.0	41.0	34.0	41.0
18	37.950442696719755	40.0	36.0	41.0	34.0	41.0
19	37.866731129644215	40.0	36.0	41.0	34.0	41.0
20	37.83965945764963	40.0	35.0	41.0	34.0	41.0
21	37.66851858798293	39.0	35.0	41.0	34.0	41.0
22	37.5856906082721	39.0	35.0	41.0	33.0	41.0
23	37.51047731778442	39.0	35.0	41.0	33.0	41.0
24	37.366740160018225	39.0	35.0	41.0	33.0	41.0
25	37.17643822598997	39.0	35.0	41.0	33.0	41.0
26	36.94948964493959	38.0	35.0	40.0	33.0	41.0
27	36.71559045447788	38.0	35.0	40.0	32.0	41.0
28	36.798644888183134	38.0	35.0	40.0	32.0	41.0
29	36.83984562228307	38.0	35.0	40.0	33.0	41.0
30	36.665438535801265	38.0	35.0	40.0	32.0	41.0
31	36.391049926853974	38.0	35.0	40.0	32.0	41.0
32	36.07714301194927	38.0	35.0	40.0	31.0	41.0
33	35.86743827739364	38.0	35.0	40.0	30.0	41.0
34	35.67656812444689	38.0	35.0	40.0	30.0	41.0
35	35.54162238310181	38.0	35.0	40.0	30.0	41.0
36	35.305530895688065	38.0	34.0	40.0	29.0	41.0
37	35.05902113524305	37.0	34.0	40.0	27.0	41.0
38	34.933532279419225	37.0	34.0	40.0	27.0	41.0
39	34.95312263386835	37.0	34.0	40.0	27.0	41.0
40	34.719147032549635	37.0	34.0	40.0	27.0	40.0
41	34.596242252981064	37.0	34.0	40.0	26.0	40.0
42	34.591499222693194	37.0	34.0	40.0	26.0	40.0
43	34.482633201489875	37.0	33.0	40.0	26.0	41.0
44	34.28140868277408	36.0	33.0	40.0	24.0	40.0
45	34.04952812822575	36.0	33.0	39.0	24.0	40.0
46	34.04294845879301	36.0	33.0	39.0	24.0	40.0
47	33.932923771139755	36.0	33.0	39.0	24.0	40.0
48	33.94121643305784	36.0	33.0	39.0	24.0	40.0
49	33.866843661997265	36.0	33.0	39.0	24.0	40.0
50	33.83646965169153	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	2.0
15	11.0
16	30.0
17	100.0
18	213.0
19	449.0
20	866.0
21	1524.0
22	2524.0
23	3979.0
24	6259.0
25	9218.0
26	12002.0
27	12990.0
28	13072.0
29	14667.0
30	18127.0
31	24972.0
32	35942.0
33	73429.0
34	66544.0
35	76938.0
36	87240.0
37	166425.0
38	76266.0
39	16003.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.57128924565813	22.64748337369216	30.553923141792154	16.22730423885756
2	30.689378751946737	24.427439555538886	31.78108150537724	13.102100187137136
3	27.95942722421585	23.932991846266912	34.471299387462786	13.636281542054451
4	25.605972828299244	27.413992633993384	33.11021363086331	13.869820906844051
5	17.16187848450874	48.30402629644912	24.102346091167878	10.431749127874264
6	21.495763365300856	37.947854452599564	29.621849615097673	10.934532567001902
7	39.30991271101553	55.48108970217827	4.181063166771557	1.027934420034649
8	88.19188294412422	2.630061698293815	7.768483438988709	1.4095719185932623
9	84.61779983967614	4.189537825458153	9.382975383200447	1.8096869516652705
10	61.041716160062684	18.300817040454685	12.80312534298055	7.854341456502077
11	57.52695566642076	15.11115001118377	18.111873830392906	9.25002049200256
12	48.14759243282444	20.704146886674362	21.245552540799924	9.902708139701275
13	19.76373763012422	48.314029172275916	21.85433867792546	10.067894519674406
14	11.797697393556202	48.4554587221604	29.464582178487426	10.282261705795973
15	10.147361810965098	20.702062954210447	58.252858808018416	10.89771642680604
16	11.523174023642909	14.186300783697536	55.34257765774326	18.94794753491629
17	13.405242896221553	14.213391905728454	31.154651406723875	41.22671379132612
18	21.03174107000207	19.127860370967763	38.463419344172564	21.376979214857606
19	31.472798429548494	20.906149406843355	26.91843349407399	20.70261866953416
20	33.81986209924242	20.49839328807032	25.17195916048086	20.509785452206398
21	21.09175832496287	27.480678472838722	29.08211110694325	22.345452095255165
22	21.897545544344	24.24863815013483	26.180165686523765	27.67365061899741
23	17.822485075570338	30.9022177209281	25.639176818890984	25.636120384610578
24	18.99059868601112	21.13677126618347	44.43082941901352	15.441800628791889
25	14.007082591800698	24.016071287161726	41.93664011736708	20.040206003670498
26	14.326479974103664	36.83697952050103	29.687979738619298	19.148560766776004
27	15.702986830936116	35.66275304149943	31.28288271767022	17.351377409894233
28	12.732271639207383	27.610160143263414	45.02475017122978	14.632818046299423
29	12.402315665753905	23.14748823620124	43.92818490871681	20.522011189328044
30	17.23328790360562	29.99265066484392	33.97115559612278	18.802905835427687
31	32.133821807102876	25.27337720705814	24.73197155293258	17.860829432906407
32	32.55588759546148	25.163345572963337	26.53179455760198	15.74897227397321
33	29.61142995277809	25.638065388243565	26.680031620201923	18.070473038776427
34	18.701626717681332	26.919128138228633	30.0915679924645	24.287677151625537
35	19.968657655742692	24.444527801743003	33.85806752774756	21.728747014766743
36	33.10076647036023	21.900324120962555	27.421078004370703	17.577831404306515
37	20.41767563730128	32.95100119062008	29.01431383745049	17.61700933462815
38	19.85612530269119	35.131489192031594	22.46034623843244	22.552039266844773
39	20.09758361084367	32.191894058430684	27.908440343265354	19.802081987460284
40	24.075116040306032	25.483576528251874	24.06330708967717	26.378000341764924
41	16.159368040533874	23.090944202013635	26.66002586854832	34.089661888904175
42	21.83933436418526	24.41521381841724	24.02218415572255	29.723267661674953
43	23.25890915860532	25.848403638268223	25.19252062745817	25.70016657566828
44	19.86862889747469	31.64909911599585	27.82605554652518	20.65621644000428
45	16.24925499414415	40.600283692672754	20.868499693661928	22.28196161952117
46	21.147190928503058	33.51213473873739	26.25741011651961	19.083264216239947
47	22.845318028933317	26.407870040414398	26.86105588690082	23.885756043751467
48	23.848800974724675	21.033825002465985	32.7960955441356	22.32127847867373
49	20.3937798783817	22.82697942325085	34.06465469933717	22.714585999030277
50	18.792486173108102	35.24582761988516	24.87270645866242	21.088979748344315
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	241.0
1	404.5
2	568.0
3	3478.0
4	6388.0
5	5006.5
6	3625.0
7	3965.0
8	4305.0
9	4842.0
10	5379.0
11	5364.0
12	5349.0
13	5066.0
14	4783.0
15	4389.5
16	3996.0
17	3777.0
18	3558.0
19	3276.5
20	2995.0
21	2892.5
22	2790.0
23	3187.0
24	3584.0
25	4160.5
26	4737.0
27	5398.5
28	6060.0
29	7132.0
30	8204.0
31	9307.5
32	10411.0
33	12545.0
34	14679.0
35	15638.5
36	16598.0
37	21199.5
38	25801.0
39	39148.5
40	52496.0
41	69182.0
42	85868.0
43	87291.0
44	88714.0
45	82466.5
46	76219.0
47	66981.0
48	57743.0
49	55167.5
50	52592.0
51	48773.5
52	44955.0
53	39048.5
54	33142.0
55	28818.5
56	24495.0
57	21798.0
58	19101.0
59	17397.5
60	15694.0
61	14187.5
62	12681.0
63	10541.0
64	8401.0
65	6700.0
66	4999.0
67	4247.0
68	3495.0
69	2907.5
70	2320.0
71	1739.5
72	1159.0
73	932.5
74	706.0
75	639.0
76	572.0
77	433.0
78	294.0
79	175.5
80	57.0
81	37.5
82	18.0
83	15.5
84	13.0
85	9.0
86	5.0
87	2.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	719793.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.85183107895239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.04331964166916	14.335558165675295
2	5.163224424169862	1.9467246933434292
3	2.0242565248818916	1.1448282620262182
4	1.195301533433903	0.901344903868348
5	0.8933305779184694	0.8420458576290949
6	0.7957412908323491	0.9000708238391384
7	0.6388786971810123	0.8430823295438336
8	0.6074716874409962	0.9161562909507059
9	0.5256340985505175	0.8918248711690592
>10	9.783651190795258	44.721843111841885
>50	2.150021845962972	27.41295621680471
>100	0.17619473633928917	4.178620565986003
>500	0.0014868754121459002	0.23713654193018002
>1k	0.0014868754121459002	0.7278073653920951
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3139	0.43609760028230343	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2054	0.2853598187256614	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	898	0.124758090173147	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	794	0.11030949175665783	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009308231672161302	0.0
2	0.0	0.0	0.0	0.03417649240823403	0.0
3	0.0	0.0	0.0	0.04529079888245648	0.0
4	0.0	0.0	0.0	0.06390726222677909	0.0
5	0.0	0.0	0.0	0.10600269799789662	0.0
6	0.0	0.0	0.0	0.1714381773648813	0.0
7	0.0	0.0	0.0	0.21283896898135993	0.0
8	0.0	0.0	0.0	0.40789504760396395	0.0
9	0.0	0.0	0.0	0.4799991108554821	0.0
10	0.0	0.0	0.0	0.5966993288348178	0.0
11	0.0	0.0	0.0	0.7175674117419869	0.0
12	0.0	0.0	0.0	0.828988334146067	0.0
13	0.0	0.0	0.0	0.8717784140718234	0.0
14	0.0	0.0	0.0	0.8888666602759404	0.0
15	0.0	0.0	0.0	0.9244324409934522	0.0
16	0.0	0.0	0.0	1.0100126008449652	0.0
17	0.0	0.0	0.0	1.1024002734119394	0.0
18	0.0	0.0	0.0	1.2549441297706423	0.0
19	0.0	0.0	0.0	1.3116270927891769	0.0
20	0.0	0.0	0.0	1.3706718459334837	0.0
21	0.0	0.0	0.0	1.450833781378813	0.0
22	0.0	0.0	0.0	1.5311346456550703	0.0
23	0.0	0.0	0.0	1.6318580480777112	0.0
24	0.0	0.0	0.0	1.7017392500343849	0.0
25	0.0	0.0	0.0	1.7559214940962193	0.0
26	0.0	0.0	0.0	1.8212180446322763	0.0
27	0.0	0.0	0.0	1.8727606409064828	0.0
28	0.0	0.0	0.0	1.9312496787270785	0.0
29	0.0	0.0	0.0	1.99626837160128	0.0
30	0.0	0.0	0.0	2.0808760296362983	0.0
31	0.0	0.0	0.0	2.1752087058362615	0.0
32	0.0	0.0	0.0	2.241477758188813	0.0
33	0.0	0.0	0.0	2.3098307430052807	0.0
34	0.0	0.0	0.0	2.392632326238238	0.0
35	0.0	0.0	0.0	2.507804327077368	0.0
36	0.0	0.0	0.0	2.59532949056187	0.0
37	1.3892883092778062E-4	0.0	0.0	2.6824378675535883	0.0
38	1.3892883092778062E-4	0.0	0.0	2.7577372939164455	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTACG	30	2.5263926E-6	44.000004	1
GAGTACG	30	2.5263926E-6	44.000004	1
TGACGTA	30	2.5263926E-6	44.000004	13
CGGATGG	30	2.5263926E-6	44.000004	2
CGAATTT	30	2.5263926E-6	44.000004	21
CGATGCG	30	2.5263926E-6	44.000004	10
ATCGCAA	30	2.5263926E-6	44.000004	1
CGGACTC	30	2.5263926E-6	44.000004	28
CTAGCGA	30	2.5263926E-6	44.000004	1
TCACGAC	25	4.4411754E-5	44.0	25
TATCACG	20	7.854519E-4	44.0	1
AAGTAAC	20	7.854519E-4	44.0	42
ACTATCG	25	4.4411754E-5	44.0	13
GATCGTA	25	4.4411754E-5	44.0	21
CCGATTC	25	4.4411754E-5	44.0	38
CGACGGT	25	4.4411754E-5	44.0	28
CGCGGAC	25	4.4411754E-5	44.0	26
GAGTCAC	20	7.854519E-4	44.0	9
ACCGCAA	20	7.854519E-4	44.0	28
TAGCGGC	25	4.4411754E-5	44.0	43
>>END_MODULE