FastQCFastQC Report
Thu 26 May 2016
SRR1546398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546398_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences265774
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11750.44210494630776526No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.20092258836455035No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4120.15501892585429727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT253.8826864E-545.00000444
TTAGGAT700.045.0000045
TTCACTA253.8826864E-545.00000416
CGATTGA253.8826864E-545.00000410
CGCGAAG253.8826864E-545.0000042
CGGGCCA253.8826864E-545.00000420
TAAGGCG502.1827873E-1145.0000045
AGGATCC700.045.0000047
AGTAGCG253.8826864E-545.0000041
TACACGG253.8826864E-545.0000042
TCACGAC207.02349E-445.025
AACCGGT207.02349E-445.016
GATCGTT207.02349E-445.09
TCACCCG207.02349E-445.014
CGACGGT207.02349E-445.028
GTCCTCG207.02349E-445.041
GGACTAC207.02349E-445.08
ATTCGTA207.02349E-445.01
CAATTAG207.02349E-445.02
CGATTGC207.02349E-445.010