FastQCFastQC Report
Thu 26 May 2016
SRR1546397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546397_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences178251
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.26367313507357604No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4350.24403790161065014No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.12005542745903248No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.10098120066647591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC351.4368925E-744.021
TCACGAC207.8385725E-444.025
TTGATTC302.5156514E-644.043
ATGGGAC408.245479E-944.05
TCGTTGA551.8189894E-1244.024
CTCACGA207.8385725E-444.024
ACACGTG351.4368925E-744.042
ACACGAC351.4368925E-744.026
CATATCA351.4368925E-744.029
TTAATCG551.8189894E-1244.020
TTTACGG207.8385725E-444.02
CACGGGA207.8385725E-444.04
ACTTGCG207.8385725E-444.013
TAGACCG207.8385725E-444.022
GCGAAAA207.8385725E-444.01
GCGCGAC207.8385725E-444.09
AGCCATT207.8385725E-444.012
TTAGACC207.8385725E-444.021
CGTTGAT551.8189894E-1244.025
TAGCGAA207.8385725E-444.01
Produced by FastQC (version 0.11.5)