FastQCFastQC Report
Thu 26 May 2016
SRR1546396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546396_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences67022
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1750.2611082927993793No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.19545820775267822No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.15815702306705262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGCC453.7289283E-1045.00000410
GTATGGC453.7289283E-1045.0000049
CTTAAGG253.8514787E-545.03
TAATATT206.985775E-445.036
CTATGCT206.985775E-445.036
TGATAAG206.985775E-445.02
TCGTTGA206.985775E-445.024
CCGGGGG206.985775E-445.05
TTAATCG206.985775E-445.020
CTGTACA206.985775E-445.016
GTACACT206.985775E-445.018
TTCAGAG206.985775E-445.02
CGAAAGT206.985775E-445.014
GTTTCAG206.985775E-445.019
AGGAGCC206.985775E-445.07
GGCACCG206.985775E-445.08
GGACTTG206.985775E-445.08
CATGAAG206.985775E-445.02
CACATAG206.985775E-445.034
CGTTGAT206.985775E-445.025