FastQCFastQC Report
Thu 26 May 2016
SRR1546395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546395_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences871842
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28840.3307938823777703No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12990.14899488668818434No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.14050711023327622No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8900.10208271682254352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT408.309144E-944.0000044
AATTCGC207.8554335E-444.00000413
TCGACGC207.8554335E-444.00000416
GGTTACG207.8554335E-444.0000041
CGACGGT502.7284841E-1144.028
GACCGTA254.4419525E-544.09
CTAACGG254.4419525E-544.02
CGTTTTA15900.043.3081741
AGGCGAT7200.041.5555577
AACGGGA3150.041.206354
CGTTATT9650.041.036271
CGTTTTT9250.040.1945951
GCGATTC1100.040.09
AGCGTTA452.3497705E-839.111111
CGTATGG1150.038.260872
TTAGGAT3750.038.133335
TAAACGG750.038.133332
GGATGCC9250.038.0540548
GTTTTAT19900.038.0301482
TTTACGG1050.037.7142872
Produced by FastQC (version 0.11.5)