##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546395_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	871842
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.529734745515817	31.0	31.0	33.0	30.0	34.0
2	31.732445787195385	31.0	31.0	34.0	30.0	34.0
3	31.811177942792387	31.0	31.0	34.0	30.0	34.0
4	35.71072052046128	37.0	35.0	37.0	35.0	37.0
5	27.179506149049942	35.0	26.0	37.0	0.0	37.0
6	31.389360686913456	35.0	28.0	37.0	17.0	37.0
7	14.922378137323047	0.0	0.0	32.0	0.0	37.0
8	25.05603997054512	17.0	17.0	35.0	17.0	37.0
9	33.17016730095591	32.0	32.0	37.0	28.0	39.0
10	35.99761998160217	37.0	35.0	39.0	32.0	39.0
11	37.000548264479114	39.0	37.0	39.0	34.0	39.0
12	37.13037109935057	39.0	37.0	39.0	34.0	39.0
13	36.80402068264662	39.0	35.0	39.0	33.0	39.0
14	38.18107982868455	40.0	37.0	41.0	33.0	41.0
15	38.40378761289316	40.0	37.0	41.0	34.0	41.0
16	38.40721828037649	40.0	37.0	41.0	34.0	41.0
17	38.39827170519429	40.0	37.0	41.0	34.0	41.0
18	38.366869226304765	40.0	37.0	41.0	34.0	41.0
19	38.33544954246297	40.0	37.0	41.0	34.0	41.0
20	38.38071691889127	40.0	37.0	41.0	34.0	41.0
21	38.2725551189321	40.0	37.0	41.0	34.0	41.0
22	38.19646793799794	40.0	37.0	41.0	34.0	41.0
23	38.09429575542357	40.0	36.0	41.0	34.0	41.0
24	37.96834288781683	40.0	36.0	41.0	34.0	41.0
25	37.79698156317314	40.0	35.0	41.0	33.0	41.0
26	37.55275153066725	39.0	35.0	41.0	33.0	41.0
27	37.3676388611698	39.0	35.0	40.0	33.0	41.0
28	37.4064887904001	39.0	35.0	40.0	33.0	41.0
29	37.37794577457842	39.0	35.0	40.0	33.0	41.0
30	37.16685248015122	39.0	35.0	40.0	32.0	41.0
31	37.014991248414276	39.0	35.0	40.0	32.0	41.0
32	36.85905358998534	39.0	35.0	40.0	32.0	41.0
33	36.69112981480589	39.0	35.0	40.0	31.0	41.0
34	36.47070340726875	39.0	35.0	40.0	31.0	41.0
35	36.31974027404048	39.0	35.0	40.0	30.0	41.0
36	36.157350758509	38.0	35.0	40.0	30.0	41.0
37	36.04412611459416	38.0	35.0	40.0	30.0	41.0
38	35.89379727060637	38.0	35.0	40.0	30.0	41.0
39	35.81067670518282	38.0	35.0	40.0	30.0	41.0
40	35.608603393734185	38.0	35.0	40.0	29.0	41.0
41	35.415889576322314	38.0	34.0	40.0	29.0	41.0
42	35.413010614308554	38.0	34.0	40.0	29.0	41.0
43	35.31200492749833	38.0	34.0	40.0	28.0	41.0
44	35.07682814087874	38.0	34.0	40.0	27.0	40.0
45	34.87608075775198	37.0	34.0	40.0	27.0	40.0
46	34.80634908618764	37.0	34.0	40.0	27.0	40.0
47	34.67066739156866	37.0	34.0	40.0	27.0	40.0
48	34.583025364687636	37.0	34.0	39.0	26.0	40.0
49	34.41419546202179	36.0	33.0	39.0	26.0	40.0
50	34.54554150866785	37.0	34.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	3.0
13	1.0
14	5.0
15	19.0
16	32.0
17	95.0
18	269.0
19	475.0
20	876.0
21	1458.0
22	2368.0
23	3642.0
24	5769.0
25	8416.0
26	10874.0
27	12462.0
28	13618.0
29	15602.0
30	19362.0
31	25590.0
32	35886.0
33	60912.0
34	68179.0
35	89872.0
36	127513.0
37	237783.0
38	102618.0
39	28140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.462581522798853	23.81234214456289	29.309324395934127	15.415751936704128
2	31.350175834612237	23.976592088933547	31.48173636966331	13.19149570679091
3	29.204144787702358	23.88024435620216	33.82963885658181	13.085971999513674
4	26.94089066596929	27.01429846233607	31.59333916007717	14.451471711617472
5	17.545036830067833	48.15104112901191	23.60163882905389	10.70228321186637
6	22.11776904530867	40.57180085382443	26.81345931946385	10.496970781403052
7	39.6071765296923	55.941214119072036	3.4268823938282393	1.02472695740742
8	88.70013144583537	2.8780444163047894	6.83931262774677	1.582511510113071
9	83.37347822197141	5.2797410539983165	8.440061387269711	2.9067193367605597
10	42.28816689262504	33.17229498005372	13.695027309994243	10.844510817326993
11	35.88586005262421	21.86978833320716	25.597413292775528	16.646938321393094
12	33.126529807006314	21.31108618304693	28.15303690347563	17.409347106471127
13	21.304318901819368	31.181682002014128	29.10997634892561	18.4040227472409
14	17.194055803689203	32.877746197132055	30.89493279745642	19.03326520172233
15	17.08910559482108	24.209547142716225	41.20115800798769	17.500189254475007
16	19.639911818884613	21.49162348223646	38.5218881402823	20.346576558596627
17	19.592999649018974	21.80418011520436	29.91367701945995	28.68914321631672
18	22.127977316990926	23.805116064608036	32.34714546901847	21.71976114938257
19	27.051346459564922	24.330899406085045	27.33281947875877	21.284934655591265
20	29.05090601278672	22.54812225150887	27.593073056815342	20.807898678889064
21	24.614322319869885	24.945460301293124	28.748442951819253	21.69177442701774
22	24.421970953452576	23.09569853253227	27.601790232633896	24.880540281381258
23	22.08278564235263	26.475439357131226	28.233326680751787	23.20844831976436
24	20.930971437485233	23.445073763365382	35.720692510798976	19.903262288350412
25	20.425031140963615	25.815457387921203	32.25940021242381	21.50011125869137
26	20.465634828328984	29.26952360634152	27.990278054968677	22.274563510360824
27	19.542875888062287	28.7258471145001	29.94843102305234	21.782845974385268
28	18.43820325242418	26.771938034643895	34.31986529669367	20.469993416238264
29	19.48277325478699	23.37613925458971	33.94147104635932	23.199616444263985
30	21.19363370886009	26.5126020540419	30.963064408459328	21.330699828638675
31	26.175958487891155	25.8770511170602	26.760238667097937	21.186751727950707
32	26.60218250554573	25.619435631685555	27.65764897768174	20.120732885086976
33	25.956193897518126	26.730760848869405	26.623287247001176	20.68975800661129
34	21.512384124646438	25.98980090429229	29.322400159661953	23.175414811399314
35	22.4269993875037	25.673000383096937	30.062442506784485	21.83755772261488
36	25.97110485615513	26.693483452276904	26.740625021506194	20.594786670061776
37	22.02509170239562	29.54526164144421	27.945315779694024	20.484330876466146
38	21.819205773523183	29.7206374549517	26.671919912094168	21.788236859430953
39	22.207234797130674	28.983233200511098	26.97988855778914	21.829643444569086
40	24.15689998875943	26.219773766347572	27.19311526629825	22.430210978594744
41	19.886974933531533	25.8353004328766	28.092475471473044	26.185249162118822
42	22.19633832735748	26.18536386180065	25.559447698092086	26.058850112749788
43	22.403256553366322	26.916459633740974	26.987458736789467	23.692825076103237
44	21.055076493217808	27.762713886231676	28.37314559289413	22.809064027656387
45	20.217654116227482	30.65348996721883	25.855946375604756	23.272909540948934
46	21.12435510103895	29.92354119209673	27.564856935086862	21.387246771777455
47	21.919682694800205	26.454678714721247	28.95260838546434	22.67303020501421
48	22.390639588365783	23.93403850697718	30.404018159253628	23.27130374540341
49	20.532734142195487	25.987506910655828	31.087972361964667	22.39178658518401
50	19.940654384624736	29.55432291630823	28.785605648729927	21.719417050337103
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	247.0
1	721.5
2	1196.0
3	5484.5
4	9773.0
5	7104.0
6	4435.0
7	4410.0
8	4385.0
9	4405.0
10	4425.0
11	4333.5
12	4242.0
13	3968.0
14	3694.0
15	3573.0
16	3452.0
17	3243.0
18	3034.0
19	3108.5
20	3183.0
21	3484.0
22	3785.0
23	4398.0
24	5011.0
25	6417.5
26	7824.0
27	9872.0
28	11920.0
29	13961.0
30	16002.0
31	17871.0
32	19740.0
33	23544.5
34	27349.0
35	29764.0
36	32179.0
37	34900.0
38	37621.0
39	44125.0
40	50629.0
41	57244.5
42	63860.0
43	65661.5
44	67463.0
45	67237.0
46	67011.0
47	65851.5
48	64692.0
49	63969.5
50	63247.0
51	58454.0
52	53661.0
53	50066.0
54	46471.0
55	44205.5
56	41940.0
57	40441.5
58	38943.0
59	36501.5
60	34060.0
61	30299.0
62	26538.0
63	22280.5
64	18023.0
65	14866.0
66	11709.0
67	9899.0
68	8089.0
69	6775.5
70	5462.0
71	4307.0
72	3152.0
73	2447.0
74	1742.0
75	1283.0
76	824.0
77	648.5
78	473.0
79	342.5
80	212.0
81	152.0
82	92.0
83	60.0
84	28.0
85	20.0
86	12.0
87	8.0
88	4.0
89	5.0
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	871842.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.847190504596092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.4902646257605	16.27249081352682
2	6.782789928381989	3.3706654700632597
3	3.7027030458631462	2.7600530388752937
4	2.8131022395099254	2.795907490160361
5	2.2849544275993035	2.838734897844011
6	2.0444657323272186	3.0479537718751777
7	1.7445493384946449	3.0343004830770473
8	1.5089573198375166	2.999467999144601
9	1.3646600297736498	3.0517170960414286
>10	12.105115187887918	56.51275106510543
>50	0.14740762222663156	2.2125488167892864
>100	0.009024956458004295	0.31880137497297173
>500	5.013864698891275E-4	0.11087660169048257
>1k	0.0015041594096673826	0.6737310808338535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2884	0.3307938823777703	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1299	0.14899488668818434	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1225	0.14050711023327622	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	890	0.10208271682254352	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008717175818554279	0.0
2	0.0	0.0	0.0	0.025233930001078177	0.0
3	0.0	0.0	0.0	0.03211591091046313	0.0
4	0.0	0.0	0.0	0.044503476547356056	0.0
5	0.0	0.0	0.0	0.07765168459422693	0.0
6	0.0	0.0	0.0	0.12892244236914488	0.0
7	0.0	0.0	0.0	0.16792033418899296	0.0
8	0.0	0.0	0.0	0.36795657928844905	0.0
9	0.0	0.0	0.0	0.4503109508374224	0.0
10	0.0	0.0	0.0	0.5899004636161139	0.0
11	0.0	0.0	0.0	0.7355690595314288	0.0
12	0.0	0.0	0.0	0.8652943996733353	0.0
13	0.0	0.0	0.0	0.9171386558573686	0.0
14	0.0	0.0	0.0	0.9331966113126002	0.0
15	0.0	0.0	0.0	0.9646243241321248	0.0
16	0.0	0.0	0.0	1.0325265357713898	0.0
17	0.0	0.0	0.0	1.1288742685027793	0.0
18	0.0	0.0	0.0	1.2501118321897775	0.0
19	0.0	0.0	0.0	1.3152612514652884	0.0
20	0.0	0.0	0.0	1.3879808497411228	0.0
21	0.0	0.0	0.0	1.486622576108974	0.0
22	0.0	0.0	0.0	1.5901963887952175	0.0
23	0.0	0.0	0.0	1.7095987575730465	0.0
24	0.0	0.0	0.0	1.7944765221221277	0.0
25	0.0	0.0	0.0	1.867081420716139	0.0
26	0.0	0.0	0.0	1.9367041275827501	0.0
27	0.0	0.0	0.0	2.0111442210859307	0.0
28	0.0	0.0	0.0	2.079505231452488	0.0
29	0.0	0.0	0.0	2.1553217211375455	0.0
30	0.0	0.0	0.0	2.2525870513235198	0.0
31	0.0	0.0	0.0	2.3442320971001624	0.0
32	0.0	0.0	0.0	2.4380564368314444	0.0
33	0.0	0.0	0.0	2.532224875608195	0.0
34	0.0	0.0	0.0	2.6314401003851615	0.0
35	0.0	0.0	0.0	2.7515306672539293	0.0
36	0.0	0.0	0.0	2.8521222882127724	0.0
37	0.0	0.0	0.0	2.963725078626632	0.0
38	0.0	0.0	0.0	3.0614492075398982	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	40	8.309144E-9	44.000004	4
AATTCGC	20	7.8554335E-4	44.000004	13
TCGACGC	20	7.8554335E-4	44.000004	16
GGTTACG	20	7.8554335E-4	44.000004	1
CGACGGT	50	2.7284841E-11	44.0	28
GACCGTA	25	4.4419525E-5	44.0	9
CTAACGG	25	4.4419525E-5	44.0	2
CGTTTTA	1590	0.0	43.308174	1
AGGCGAT	720	0.0	41.555557	7
AACGGGA	315	0.0	41.20635	4
CGTTATT	965	0.0	41.03627	1
CGTTTTT	925	0.0	40.194595	1
GCGATTC	110	0.0	40.0	9
AGCGTTA	45	2.3497705E-8	39.11111	1
CGTATGG	115	0.0	38.26087	2
TTAGGAT	375	0.0	38.13333	5
TAAACGG	75	0.0	38.13333	2
GGATGCC	925	0.0	38.054054	8
GTTTTAT	1990	0.0	38.030148	2
TTTACGG	105	0.0	37.714287	2
>>END_MODULE