FastQCFastQC Report
Thu 26 May 2016
SRR1546394_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546394_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences334006
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7620.2281396142584265No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.22454686442758515No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3520.10538732837134662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG253.8848433E-545.0000042
GCCGATT253.8848433E-545.0000049
GTATAGA253.8848433E-545.0000041
CGCAAAG253.8848433E-545.0000042
TTAGGAC1450.045.05
ACGCCGG207.0260925E-445.027
GCACCTA207.0260925E-445.09
ATCGTAG207.0260925E-445.02
TCGTGCA207.0260925E-445.01
TACGCAA207.0260925E-445.01
CCATAAC207.0260925E-445.029
GCGATTC351.2084638E-745.09
CTTCTCG207.0260925E-445.013
CGGTCTA207.0260925E-445.031
CGTTTTA4300.043.4302371
CAAGGAT1900.042.631585
CGTTTTT6500.042.5769231
GGACCGA1350.041.6666688
GAGGTAT603.6379788E-1241.2499966
TAGGCGA603.6379788E-1241.2499966