##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546394_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	334006
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.012167446093784	31.0	31.0	33.0	30.0	33.0
2	31.17754172080741	31.0	31.0	33.0	30.0	34.0
3	31.146392579774016	31.0	31.0	34.0	28.0	34.0
4	35.12908750142213	35.0	35.0	37.0	33.0	37.0
5	32.848095543193836	35.0	35.0	37.0	28.0	37.0
6	34.035484392495945	35.0	35.0	37.0	28.0	37.0
7	15.200241911821943	0.0	0.0	35.0	0.0	37.0
8	24.85825703729873	17.0	17.0	35.0	17.0	37.0
9	32.65163500056885	32.0	32.0	37.0	27.0	39.0
10	35.4355879834494	37.0	34.0	37.0	32.0	39.0
11	36.4333934120944	37.0	35.0	39.0	32.0	39.0
12	36.40507056759459	37.0	35.0	39.0	32.0	39.0
13	36.28401884996078	38.0	35.0	39.0	32.0	39.0
14	37.385457147476394	39.0	36.0	40.0	32.0	41.0
15	37.648907504655604	39.0	36.0	41.0	33.0	41.0
16	37.60957587588247	39.0	36.0	41.0	33.0	41.0
17	37.558295359963594	39.0	36.0	41.0	32.0	41.0
18	37.47067717346395	39.0	36.0	41.0	32.0	41.0
19	37.44177050711664	39.0	36.0	41.0	32.0	41.0
20	37.57256755866661	39.0	36.0	41.0	32.0	41.0
21	37.46487188852895	39.0	36.0	41.0	32.0	41.0
22	37.51601468237097	39.0	36.0	41.0	32.0	41.0
23	37.483305689119355	39.0	35.0	41.0	33.0	41.0
24	37.33008089675036	39.0	35.0	41.0	32.0	41.0
25	37.07381304527463	39.0	35.0	40.0	32.0	41.0
26	36.958821099022174	39.0	35.0	40.0	31.0	41.0
27	36.90237001730508	39.0	35.0	40.0	31.0	41.0
28	36.81851823021143	39.0	35.0	40.0	31.0	41.0
29	36.82292833062879	39.0	35.0	40.0	31.0	41.0
30	36.555513972802885	38.0	35.0	40.0	30.0	41.0
31	36.46997658724693	38.0	35.0	40.0	30.0	41.0
32	36.40927408489668	38.0	35.0	40.0	30.0	41.0
33	36.24066932929349	38.0	35.0	40.0	30.0	41.0
34	36.105599899403	38.0	35.0	40.0	30.0	41.0
35	35.94921348718287	38.0	35.0	40.0	29.0	41.0
36	35.75052244570457	38.0	35.0	40.0	28.0	41.0
37	35.67634114357227	38.0	35.0	40.0	28.0	41.0
38	35.59234564648539	38.0	34.0	40.0	27.0	41.0
39	35.469060436040074	38.0	34.0	40.0	27.0	41.0
40	35.340601665838335	38.0	34.0	40.0	26.0	41.0
41	35.205089130135384	38.0	34.0	40.0	26.0	41.0
42	35.15404513691371	38.0	34.0	40.0	26.0	41.0
43	35.100261671946015	38.0	34.0	40.0	26.0	41.0
44	34.87600821542128	38.0	34.0	40.0	25.0	41.0
45	34.818988281647634	38.0	34.0	40.0	24.0	41.0
46	34.76327670760406	38.0	34.0	40.0	24.0	41.0
47	34.708433381436265	38.0	33.0	40.0	24.0	41.0
48	34.66085040388496	37.0	33.0	40.0	24.0	41.0
49	34.52603246648264	37.0	33.0	40.0	24.0	41.0
50	34.43528259971378	37.0	33.0	40.0	24.0	41.0
51	34.26488745711155	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	3.0
15	9.0
16	33.0
17	88.0
18	170.0
19	291.0
20	519.0
21	878.0
22	1374.0
23	2007.0
24	3001.0
25	4057.0
26	4864.0
27	5551.0
28	6249.0
29	7327.0
30	9099.0
31	11638.0
32	15679.0
33	22454.0
34	28903.0
35	33470.0
36	42012.0
37	65137.0
38	56507.0
39	12683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.920103231678475	24.56303180182392	29.368035304755004	15.148829661742603
2	31.026688143326766	23.87861295904864	32.01589193008509	13.078806967539505
3	29.567732316185936	23.594486326592936	33.80298557510943	13.034795782111699
4	27.36208331586858	27.528547391364228	30.44526146236894	14.664107830398256
5	21.559193547421305	36.68287396034802	28.632419776890234	13.125512715340443
6	22.254390639689106	41.033993401316145	26.33874840571726	10.372867553277485
7	38.497811416561376	57.04568181409915	3.6804728058777387	0.7760339634617341
8	89.14570396938977	3.0634180224307346	6.310066286234379	1.4808117219451147
9	83.62484506266354	5.459482763782686	7.915426668981993	3.000245504571774
10	42.162116848200334	33.83621851104471	13.23928312665042	10.762381514104536
11	36.21731346143483	22.13882385346371	25.21661287521781	16.427249809883655
12	33.31976072286126	21.661586917600285	27.868661042017205	17.149991317521245
13	21.41548355418765	31.776075878876426	28.582720070896926	18.225720496038996
14	17.066160488134944	33.573049585935586	30.322209780662625	19.038580145266852
15	16.948797326994125	24.619018820021196	40.9148338652599	17.51734998772477
16	19.4972545403376	21.85170326281564	38.257695969533486	20.393346227313284
17	19.40324425309725	22.19181691346862	29.693478560265383	28.711460273168743
18	22.12834499979042	24.060046825506127	32.228163565923964	21.583444608779484
19	27.196816823649872	24.60824057052867	26.988437333461075	21.206505272360378
20	29.523122339119656	22.75348347035682	27.15999113788375	20.56340305263977
21	24.498062909049537	25.469901738292126	28.32074872906475	21.711286623593587
22	24.33219762519236	23.325628880918305	27.287234361059383	25.05493913282995
23	22.069064627581543	26.704909492643846	28.07973509457914	23.146290785195475
24	20.822679832098824	23.866038334640695	35.70504721472069	19.606234618539787
25	20.300832919169117	26.08366316772753	32.21049921258899	21.40500470051436
26	20.44244714166811	29.983293713286585	27.683335029909646	21.890924115135658
27	19.410130356939696	29.506954964880872	29.54767279629707	21.53524188188236
28	18.323024137290947	27.260887528966542	34.241001658652834	20.175086675089666
29	19.347856026538444	23.763944360280952	33.764662910247125	23.12353670293348
30	20.999023969629288	27.02316724849254	30.678191409735156	21.299617372143015
31	26.456410962677317	26.054921169080796	26.452219421208	21.036448447033887
32	27.170469991557038	25.625886960114492	27.321664880271612	19.88197816805686
33	26.314497344359083	26.85939773537002	26.33126351023634	20.49484141003455
34	21.50350592504326	26.07587887642737	29.129716232642526	23.290898965886843
35	22.540912438698708	25.683071561588715	30.054849314084176	21.7211666856284
36	26.046238690322927	26.89412765040149	26.58724693568379	20.472386723591793
37	21.84870930462327	30.010538732837134	27.857882792524684	20.282869170014912
38	21.650209876469283	30.30185086495452	26.38635234097591	21.661586917600285
39	22.037328670742443	29.62401873020245	26.865685047573994	21.472967551481112
40	24.349562582708096	26.6255696005461	26.777662676718382	22.247205140027422
41	19.92389358275001	25.818099076064506	27.96267132925756	26.29533601192793
42	22.093914480578196	26.17078735112543	25.381280575798037	26.35401759249834
43	22.271156805566367	27.055501996970115	26.77496811434525	23.89837308311827
44	21.00710765674868	27.893211499194624	28.52882882343431	22.570852020622382
45	20.137961593504308	31.150638012490795	25.598342544744707	23.113057849260194
46	20.905911869846648	30.588672059783356	27.300707172925037	21.204708897444956
47	21.74930989263666	26.816284737399926	28.71565181463806	22.718753555325353
48	22.223253474488484	24.096872511272252	30.60543822566062	23.074435788578647
49	20.493943222576842	26.235157452261337	31.344047711717753	21.92685161344407
50	19.84784704466387	30.171613683586525	28.471644221960084	21.508895049789526
51	19.6795865942528	30.08718406256175	27.21597815608103	23.017251187104424
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	85.0
1	255.0
2	425.0
3	1756.5
4	3088.0
5	2344.5
6	1601.0
7	1511.0
8	1421.0
9	1460.0
10	1499.0
11	1473.0
12	1447.0
13	1381.5
14	1316.0
15	1248.5
16	1181.0
17	1130.0
18	1079.0
19	1140.0
20	1201.0
21	1308.0
22	1415.0
23	1556.5
24	1698.0
25	2153.0
26	3456.0
27	4304.0
28	5016.0
29	5728.0
30	6395.0
31	7062.0
32	8262.0
33	9462.0
34	10702.5
35	11943.0
36	13095.0
37	14247.0
38	16138.5
39	18030.0
40	20717.5
41	23405.0
42	24424.5
43	25444.0
44	25173.0
45	24902.0
46	24424.0
47	23946.0
48	23932.5
49	23919.0
50	23100.0
51	22281.0
52	20421.5
53	18562.0
54	17490.0
55	16418.0
56	15937.0
57	15456.0
58	14604.5
59	13753.0
60	12439.5
61	11126.0
62	9751.0
63	8376.0
64	6907.5
65	5439.0
66	4550.5
67	3662.0
68	3126.0
69	2590.0
70	2137.5
71	1685.0
72	1389.0
73	1093.0
74	846.5
75	433.5
76	267.0
77	196.0
78	125.0
79	96.0
80	67.0
81	49.5
82	32.0
83	21.0
84	10.0
85	7.0
86	4.0
87	2.5
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	334006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.0704733985366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.990546962252495	23.35089395523451
2	9.760350885648174	7.236416557336075
3	6.102393666554068	6.786558662501724
4	4.371010791583269	6.48141757096416
5	3.3752920712348264	6.256183746950105
6	2.5705754291428273	5.717546883898258
7	2.220079916090427	5.760958943043889
8	1.7356728783289483	5.147377221172379
9	1.4353623340076438	4.788860509808258
>10	5.425068124342316	27.61911497606759
>50	0.009633134692965931	0.23063022426632335
>100	0.0024082836732414827	0.17408862089780536
>500	0.001605522448827655	0.4499521278589431
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	762	0.2281396142584265	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	750	0.22454686442758515	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	352	0.10538732837134662	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002993958192367802	0.0
2	0.0	0.0	0.0	0.022754082261995292	0.0
3	0.0	0.0	0.0	0.03293354011604582	0.0
4	0.0	0.0	0.0	0.045508164523990584	0.0
5	0.0	0.0	0.0	0.07933989209774675	0.0
6	0.0	0.0	0.0	0.11975832769471208	0.0
7	0.0	0.0	0.0	0.14430878487212803	0.0
8	0.0	0.0	0.0	0.30478494398304223	0.0
9	0.0	0.0	0.0	0.3823284611653683	0.0
10	0.0	0.0	0.0	0.4952006850176344	0.0
11	0.0	0.0	0.0	0.6155578043508201	0.0
12	0.0	0.0	0.0	0.7098674874104058	0.0
13	0.0	0.0	0.0	0.7532798811997389	0.0
14	0.0	0.0	0.0	0.7679502763423411	0.0
15	0.0	0.0	0.0	0.7963928791698353	0.0
16	0.0	0.0	0.0	0.8589666053903223	0.0
17	0.0	0.0	0.0	0.935611935114938	0.0
18	0.0	0.0	0.0	1.0368077220169698	0.0
19	0.0	0.0	0.0	1.0909983652988269	0.0
20	0.0	0.0	0.0	1.1484823625922889	0.0
21	0.0	0.0	0.0	1.2248282964976678	0.0
22	0.0	0.0	0.0	1.31853918791878	0.0
23	0.0	0.0	0.0	1.4158428291707335	0.0
24	0.0	0.0	0.0	1.4891948048837447	0.0
25	0.0	0.0	0.0	1.5574570516697306	0.0
26	0.0	0.0	0.0	1.6200307778902174	0.0
27	0.0	0.0	0.0	1.6808081291952839	0.0
28	0.0	0.0	0.0	1.7523637299928745	0.0
29	0.0	0.0	0.0	1.8254163098866487	0.0
30	0.0	0.0	0.0	1.9224205553193656	0.0
31	0.0	0.0	0.0	2.00385621815177	0.0
32	0.0	0.0	0.0	2.0927767764650933	0.0
33	0.0	0.0	0.0	2.175410022574445	0.0
34	0.0	0.0	0.0	2.2658275599839524	0.0
35	0.0	0.0	0.0	2.3637299928743793	0.0
36	0.0	0.0	0.0	2.4598360508493857	0.0
37	0.0	0.0	0.0	2.5601336502937073	0.0
38	0.0	0.0	0.0	2.6571378957264242	0.0
39	0.0	0.0	0.0	2.753842745339904	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	25	3.8848433E-5	45.000004	2
GCCGATT	25	3.8848433E-5	45.000004	9
GTATAGA	25	3.8848433E-5	45.000004	1
CGCAAAG	25	3.8848433E-5	45.000004	2
TTAGGAC	145	0.0	45.0	5
ACGCCGG	20	7.0260925E-4	45.0	27
GCACCTA	20	7.0260925E-4	45.0	9
ATCGTAG	20	7.0260925E-4	45.0	2
TCGTGCA	20	7.0260925E-4	45.0	1
TACGCAA	20	7.0260925E-4	45.0	1
CCATAAC	20	7.0260925E-4	45.0	29
GCGATTC	35	1.2084638E-7	45.0	9
CTTCTCG	20	7.0260925E-4	45.0	13
CGGTCTA	20	7.0260925E-4	45.0	31
CGTTTTA	430	0.0	43.430237	1
CAAGGAT	190	0.0	42.63158	5
CGTTTTT	650	0.0	42.576923	1
GGACCGA	135	0.0	41.666668	8
GAGGTAT	60	3.6379788E-12	41.249996	6
TAGGCGA	60	3.6379788E-12	41.249996	6
>>END_MODULE