FastQCFastQC Report
Thu 26 May 2016
SRR1546393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546393_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1030919
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA63230.6133362562917164No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36870.357642064992497No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14950.1450162427892007No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13940.13521915882819116No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTCG207.856103E-444.00000433
AAGTACC650.044.00000433
CAGCGAA207.856103E-444.0000041
AATCCGT207.856103E-444.00000410
GTCGCGA207.856103E-444.00000417
GTACCAT650.044.0000049
GATCGTA207.856103E-444.00000433
ATAATAC207.856103E-444.0000043
GACGTCC207.856103E-444.00000435
TTAGACG551.8189894E-1244.0000041
GAGTAGA207.856103E-444.0000041
CCAATCC207.856103E-444.0000048
CAACCGT207.856103E-444.00000421
GTGATTA551.8189894E-1244.0000041
ATTAACG207.856103E-444.0000041
TGTATCG207.856103E-444.0000041
TAACGTA207.856103E-444.0000041
CCTGTCG207.856103E-444.00000425
TATGCGT207.856103E-444.00000420
TTATCGT207.856103E-444.00000425
Produced by FastQC (version 0.11.5)