FastQCFastQC Report
Thu 26 May 2016
SRR1546392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546392_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences398689
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24620.617523934695966No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.27088783487881535No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.21671026790305226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAGA351.2092642E-745.0000041
GGACTAC302.1619217E-645.0000048
TGGCGAC302.1619217E-645.00000429
ACCGAAT351.2092642E-745.00000425
CATTAGG700.045.0000043
GTGCTTA302.1619217E-645.0000041
AGGCATA302.1619217E-645.0000041
CATAGGC302.1619217E-645.0000044
AGATTAA351.2092642E-745.0000041
TGCTACC351.2092642E-745.00000421
ACCCGAG302.1619217E-645.00000444
GCTACCG351.2092642E-745.00000422
AGGATTC700.045.0000047
GTGACCC302.1619217E-645.00000441
CGATAAG302.1619217E-645.0000042
GTAGTAA302.1619217E-645.0000041
GACTAAG351.2092642E-745.0000042
AAGTACG207.027736E-445.01
GGCAGTC207.027736E-445.08
TTAGGCC453.8380676E-1045.05