##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546392_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	398689
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.118656396339002	31.0	31.0	33.0	30.0	34.0
2	31.25370150668817	31.0	31.0	33.0	30.0	34.0
3	31.142930956208975	31.0	31.0	34.0	28.0	34.0
4	35.111821494949695	35.0	35.0	37.0	33.0	37.0
5	32.861726809618524	35.0	35.0	37.0	28.0	37.0
6	34.05086922388127	35.0	35.0	37.0	28.0	37.0
7	15.326414824587586	0.0	0.0	35.0	0.0	37.0
8	24.970538439736234	17.0	17.0	35.0	17.0	37.0
9	32.9181893656459	32.0	32.0	37.0	28.0	39.0
10	35.803814502030406	37.0	35.0	38.0	32.0	39.0
11	36.68557697854719	38.0	35.0	39.0	33.0	39.0
12	36.13613618635076	37.0	35.0	39.0	32.0	39.0
13	35.330483158552155	37.0	35.0	39.0	30.0	39.0
14	36.279368129042936	38.0	35.0	40.0	30.0	41.0
15	36.907062898650324	39.0	35.0	41.0	31.0	41.0
16	37.15036281412329	39.0	35.0	41.0	32.0	41.0
17	37.183388555992266	39.0	35.0	41.0	32.0	41.0
18	37.053297181512406	38.0	35.0	40.0	32.0	41.0
19	36.91455495386128	38.0	35.0	40.0	32.0	41.0
20	36.9885449560936	38.0	35.0	40.0	32.0	41.0
21	36.7795499750432	38.0	35.0	40.0	32.0	41.0
22	36.819992525502336	38.0	35.0	40.0	32.0	41.0
23	36.83243580836191	38.0	35.0	40.0	32.0	41.0
24	36.658076846865605	38.0	35.0	40.0	32.0	41.0
25	36.42945754711066	38.0	35.0	40.0	31.0	41.0
26	36.2729220018611	38.0	35.0	40.0	31.0	41.0
27	36.09401814446849	37.0	34.0	40.0	31.0	41.0
28	36.113411706869265	37.0	35.0	40.0	31.0	41.0
29	36.231034716282615	38.0	35.0	40.0	31.0	41.0
30	36.025164978216104	37.0	35.0	40.0	31.0	41.0
31	35.790942313432275	37.0	34.0	40.0	30.0	41.0
32	35.54921505233403	37.0	34.0	40.0	30.0	41.0
33	35.289461209112865	37.0	34.0	40.0	29.0	41.0
34	35.110168577512795	37.0	34.0	40.0	27.0	41.0
35	34.89373170566532	37.0	34.0	40.0	26.0	41.0
36	34.653225948044714	37.0	34.0	40.0	24.0	41.0
37	34.42824356829509	36.0	33.0	40.0	23.0	41.0
38	34.44982429914043	37.0	33.0	40.0	24.0	41.0
39	34.38059489978404	36.0	33.0	40.0	23.0	41.0
40	34.205638981762725	36.0	33.0	40.0	23.0	41.0
41	34.15875030412176	36.0	33.0	40.0	23.0	41.0
42	34.095457863146464	36.0	33.0	40.0	23.0	41.0
43	33.96617915217124	36.0	33.0	40.0	23.0	41.0
44	33.73303502228554	35.0	33.0	40.0	22.0	41.0
45	33.641462392993034	35.0	33.0	40.0	22.0	41.0
46	33.668867212288276	35.0	33.0	40.0	23.0	41.0
47	33.66944410304774	35.0	33.0	40.0	23.0	41.0
48	33.7045792585198	35.0	33.0	40.0	23.0	41.0
49	33.67189212644442	36.0	33.0	40.0	22.0	41.0
50	33.48329148785143	35.0	33.0	40.0	21.0	41.0
51	33.254313512537344	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	8.0
15	8.0
16	47.0
17	108.0
18	280.0
19	494.0
20	902.0
21	1390.0
22	2321.0
23	3543.0
24	5129.0
25	6893.0
26	8196.0
27	8553.0
28	8705.0
29	9766.0
30	11723.0
31	15670.0
32	21592.0
33	35177.0
34	44912.0
35	40717.0
36	39101.0
37	61795.0
38	62370.0
39	9286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.694044731607843	22.95021934389963	30.92585950452608	17.42987641996644
2	31.154609231757085	24.957297542696185	31.363042371372174	12.525050854174557
3	26.115593858872455	24.45841244679437	35.18682481834212	14.239168875991059
4	25.084213509778298	27.755217726097285	32.81429886452849	14.346269899595926
5	20.539568435547505	35.54424626714056	31.43904145837984	12.4771438389321
6	21.380574834018496	37.68551427302986	30.210264140721215	10.723646752230435
7	36.85052760422284	57.2255567622884	5.233151654547781	0.6907639789409791
8	87.6021159349769	2.7908971654597945	8.23774922307864	1.3692376764846785
9	85.31361537438956	3.5421092631098423	9.388019233036276	1.7562561294643193
10	66.49042235928255	14.016438878424035	12.377818299476534	7.115320462816882
11	62.891627308503615	13.712442530393364	16.276094901038153	7.119835260064862
12	54.05892813696892	19.318064957899516	18.623789469987887	7.9992174351436836
13	19.030873688514106	52.57481395273007	19.94988575054742	8.444426608208403
14	10.499161000178084	53.97716014236661	26.375947166839314	9.147731690615995
15	8.592411629114423	19.15603390111089	63.06920933359085	9.182345136183843
16	9.476308601441223	12.193213256448008	60.53665889954325	17.793819242567515
17	11.150545914233902	13.441554695514549	29.84707378432814	45.56082560592342
18	19.717122870207106	19.79638264411609	40.184203727717595	20.302290757959213
19	30.893503457582227	20.211743990930273	26.740140811509722	22.154611739977778
20	34.91869602622595	18.937567878722515	25.4265856344168	20.71715046063473
21	19.774561124084187	27.58039474377271	30.20750509795856	22.43753903418454
22	21.350225363629292	23.932187745335337	25.818620528783086	28.89896636225228
23	15.3350104969036	32.012169886803996	24.867503241875248	27.785316374417153
24	17.56632362568318	20.666484402629617	46.98348838317586	14.783703588511345
25	12.43224668852163	22.26271605185997	44.594157350716976	20.710879908901425
26	12.388352826388488	38.21249144069688	30.193459062075956	19.205696670838673
27	13.785180930499713	38.97147902249623	31.690365171850743	15.552974875153314
28	10.740451830875696	29.133234175008592	46.30250646493884	13.823807529176877
29	10.227771521160605	21.895261720288246	46.668957508233234	21.208009250317915
30	14.894566943156196	32.133567768360805	35.62676672795086	17.345098560532143
31	32.550684869660316	25.603164371226693	24.662330789161476	17.183819969951518
32	33.39971757434993	26.255301751490514	26.824417026805353	13.520563647354203
33	30.50171938528527	28.16054619013818	24.130086358038472	17.207648066538077
34	17.79783239567683	28.616791534253515	28.79663095796473	24.788745112104923
35	18.82294219303768	24.865747487389918	33.29913792454771	23.012172395024695
36	34.43009463516676	21.429485137538283	27.48357742501047	16.656842802284487
37	18.646363456227785	33.139865910521735	31.605587312416443	16.608183320834033
38	18.974438722914353	34.74337139976272	22.92438467075841	23.357805206564517
39	19.07878070375656	33.10249342219123	28.899468006390943	18.91925786766126
40	23.30488174993541	25.295154870086712	24.213860929195437	27.18610245078244
41	14.800508667156606	22.143575568927158	28.462285139544857	34.59363062437138
42	22.049015648788906	23.083155040645718	24.949020414408224	29.918808896157156
43	22.674315067634172	24.87126557291523	26.23272776525061	26.221691594199992
44	17.46022589035564	32.34776981557053	28.69128568884519	21.500718605228634
45	14.625434862762704	43.49355011048712	20.09661666110678	21.784398365643394
46	22.772386496743074	34.9703653725084	25.260541424518863	16.996706706229666
47	22.228604250430788	28.581927266616336	25.630253154714577	23.5592153282383
48	24.919172588157686	22.596560226141175	31.06280835438148	21.42145883131965
49	21.025912427982714	21.028169826606703	34.587861716776736	23.358056028633847
50	18.706811574936854	36.09354659897815	25.359114497766427	19.840527328318565
51	16.29114422519809	38.75226053389986	21.053001211470594	23.90359402943146
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	202.0
1	269.0
2	336.0
3	1924.0
4	3512.0
5	2887.5
6	2263.0
7	2494.5
8	2726.0
9	2897.5
10	3069.0
11	3185.0
12	3301.0
13	3145.5
14	2990.0
15	2804.0
16	2618.0
17	2493.5
18	2369.0
19	2113.5
20	1858.0
21	1671.0
22	1484.0
23	1546.5
24	1609.0
25	1599.0
26	1949.5
27	2310.0
28	2703.0
29	3096.0
30	3922.0
31	4748.0
32	5426.0
33	6104.0
34	6379.5
35	6655.0
36	8687.0
37	10719.0
38	18260.0
39	25801.0
40	35902.5
41	46004.0
42	50031.5
43	54059.0
44	51645.0
45	49231.0
46	43655.5
47	38080.0
48	32477.5
49	26875.0
50	25602.5
51	24330.0
52	21715.0
53	19100.0
54	16460.0
55	13820.0
56	12234.0
57	10648.0
58	9173.0
59	7698.0
60	6841.0
61	5984.0
62	5401.5
63	4819.0
64	3979.5
65	3140.0
66	2629.5
67	2119.0
68	1757.5
69	1396.0
70	1112.0
71	828.0
72	699.0
73	570.0
74	454.0
75	252.0
76	166.0
77	112.5
78	59.0
79	43.5
80	28.0
81	18.0
82	8.0
83	7.0
84	6.0
85	4.5
86	3.0
87	2.5
88	2.0
89	9.0
90	16.0
91	8.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	398689.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.627912984807708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.16028376655767	17.2334827396793
2	5.043507177298675	2.282480830923351
3	2.2645901457628996	1.537288462937277
4	1.517486005653162	1.3735016516633265
5	1.1239815995122762	1.2716678915144386
6	0.8113950008313473	1.1016105285071822
7	0.6805963531563488	1.0780332539899522
8	0.6739455744610099	1.219998545231998
9	0.5586654104084687	1.1377289064910243
>10	10.222246854735909	56.7680573078264
>50	0.8900958820595245	12.570700470793025
>100	0.04988084021504184	1.3203273729648923
>500	0.0011084631158898186	0.21671026790305226
>1k	0.0022169262317796373	0.8884117695747814
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2462	0.617523934695966	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1080	0.27088783487881535	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	864	0.21671026790305226	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010283704842621692	0.0
2	0.0	0.0	0.0	0.04088399730115454	0.0
3	0.0	0.0	0.0	0.0516693462824407	0.0
4	0.0	0.0	0.0	0.0792597739089867	0.0
5	0.0	0.0	0.0	0.12917336570610174	0.0
6	0.0	0.0	0.0	0.1861099754445194	0.0
7	0.0	0.0	0.0	0.2252382182603483	0.0
8	0.0	0.0	0.0	0.5016441386644728	0.0
9	0.0	0.0	0.0	0.6012205001893707	0.0
10	0.0	0.0	0.0	0.7707762190579625	0.0
11	0.0	0.0	0.0	0.9408335820652187	0.0
12	0.0	0.0	0.0	1.0782840760592842	0.0
13	0.0	0.0	0.0	1.1465076789176527	0.0
14	0.0	0.0	0.0	1.1693324872268862	0.0
15	0.0	0.0	0.0	1.2044475769333993	0.0
16	0.0	0.0	0.0	1.2894762584370274	0.0
17	0.0	0.0	0.0	1.3827820682286194	0.0
18	0.0	0.0	0.0	1.542555726393254	0.0
19	0.0	0.0	0.0	1.6092743968356287	0.0
20	0.0	0.0	0.0	1.678250465901994	0.0
21	0.0	0.0	0.0	1.7853514895068587	0.0
22	0.0	0.0	0.0	1.8698785268718223	0.0
23	0.0	0.0	0.0	1.9757254401300262	0.0
24	0.0	0.0	0.0	2.0401867119484107	0.0
25	0.0	0.0	0.0	2.089347837537529	0.0
26	0.0	0.0	0.0	2.1593271948812234	0.0
27	0.0	0.0	0.0	2.2112473632329963	0.0
28	0.0	0.0	0.0	2.272698770219394	0.0
29	0.0	0.0	0.0	2.336407575829782	0.0
30	0.0	0.0	0.0	2.434729827008019	0.0
31	0.0	0.0	0.0	2.5242733057596274	0.0
32	0.0	0.0	0.0	2.5987674603513016	0.0
33	0.0	0.0	0.0	2.66924846183366	0.0
34	0.0	0.0	0.0	2.7495115240199755	0.0
35	0.0	0.0	0.0	2.8821963986967285	0.0
36	0.0	0.0	0.0	2.97098741124034	0.0
37	0.0	0.0	0.0	3.0605308899919486	0.0
38	0.0	0.0	0.0	3.1305102473356428	0.0
39	0.0	0.0	0.0	3.2027470033033265	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAGA	35	1.2092642E-7	45.000004	1
GGACTAC	30	2.1619217E-6	45.000004	8
TGGCGAC	30	2.1619217E-6	45.000004	29
ACCGAAT	35	1.2092642E-7	45.000004	25
CATTAGG	70	0.0	45.000004	3
GTGCTTA	30	2.1619217E-6	45.000004	1
AGGCATA	30	2.1619217E-6	45.000004	1
CATAGGC	30	2.1619217E-6	45.000004	4
AGATTAA	35	1.2092642E-7	45.000004	1
TGCTACC	35	1.2092642E-7	45.000004	21
ACCCGAG	30	2.1619217E-6	45.000004	44
GCTACCG	35	1.2092642E-7	45.000004	22
AGGATTC	70	0.0	45.000004	7
GTGACCC	30	2.1619217E-6	45.000004	41
CGATAAG	30	2.1619217E-6	45.000004	2
GTAGTAA	30	2.1619217E-6	45.000004	1
GACTAAG	35	1.2092642E-7	45.000004	2
AAGTACG	20	7.027736E-4	45.0	1
GGCAGTC	20	7.027736E-4	45.0	8
TTAGGCC	45	3.8380676E-10	45.0	5
>>END_MODULE