FastQCFastQC Report
Thu 26 May 2016
SRR1546391_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546391_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1203079
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36650.3046350239676696No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA30880.25667474870727525No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15730.13074785612582382No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14460.12019160836487046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTA207.856628E-444.01
ATCTCGG207.856628E-444.018
ATCTCGC302.527815E-644.017
CGTATTG207.856628E-444.018
TCCGCAT207.856628E-444.041
ACGTTAG551.8189894E-1244.02
GCGAAAC207.856628E-444.025
CAGTTCG207.856628E-444.035
CGTGAAT207.856628E-444.033
CGTTGAC207.856628E-444.028
CGACCCA302.527815E-644.010
AAGCGTA207.856628E-444.015
CGACAGT254.4429646E-544.016
CTACGTC254.4429646E-544.029
TACGACT254.4429646E-544.041
GCTTATA302.527815E-644.01
TCGCTAA254.4429646E-544.01
GGTATCG254.4429646E-544.024
ACTCGTT302.527815E-644.023
TCGTATA351.4461511E-744.01
Produced by FastQC (version 0.11.5)