##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546391_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1203079
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56915298164127	31.0	31.0	33.0	30.0	34.0
2	31.759176246946378	31.0	31.0	34.0	30.0	34.0
3	31.831365188819685	31.0	31.0	34.0	30.0	34.0
4	35.71937004968086	37.0	35.0	37.0	35.0	37.0
5	27.246181672192765	35.0	26.0	37.0	0.0	37.0
6	31.426039354024134	36.0	28.0	37.0	17.0	37.0
7	14.81917147585487	0.0	0.0	32.0	0.0	37.0
8	25.004088675805995	17.0	17.0	35.0	17.0	37.0
9	33.21515129097923	32.0	32.0	37.0	28.0	39.0
10	36.1220659657429	37.0	35.0	39.0	32.0	39.0
11	37.09103142852631	39.0	37.0	39.0	34.0	39.0
12	36.990461972987646	39.0	35.0	39.0	33.0	39.0
13	36.4475608002467	39.0	35.0	39.0	33.0	39.0
14	37.76433218433702	40.0	36.0	41.0	33.0	41.0
15	38.05363820663481	40.0	36.0	41.0	33.0	41.0
16	38.14443773019062	40.0	36.0	41.0	34.0	41.0
17	38.18137129814418	40.0	36.0	41.0	34.0	41.0
18	38.14511349628744	40.0	36.0	41.0	34.0	41.0
19	38.06906445877619	40.0	36.0	41.0	34.0	41.0
20	38.09618071631206	40.0	36.0	41.0	34.0	41.0
21	37.95304547747904	40.0	36.0	41.0	34.0	41.0
22	37.87088295947315	40.0	35.0	41.0	34.0	41.0
23	37.76713249919581	40.0	35.0	41.0	34.0	41.0
24	37.668129856809074	39.0	35.0	41.0	33.0	41.0
25	37.48476118359642	39.0	35.0	41.0	33.0	41.0
26	37.23057089351572	39.0	35.0	40.0	33.0	41.0
27	37.024243628223914	39.0	35.0	40.0	33.0	41.0
28	37.07002532668262	39.0	35.0	40.0	33.0	41.0
29	37.062040813612406	39.0	35.0	40.0	33.0	41.0
30	36.88214074054987	38.0	35.0	40.0	32.0	41.0
31	36.697387287119135	38.0	35.0	40.0	32.0	41.0
32	36.4788131120234	38.0	35.0	40.0	31.0	41.0
33	36.27659280895103	38.0	35.0	40.0	31.0	41.0
34	36.07091803613894	38.0	35.0	40.0	30.0	41.0
35	35.94329383190963	38.0	35.0	40.0	30.0	41.0
36	35.71794537183344	38.0	35.0	40.0	30.0	41.0
37	35.53983902968965	38.0	35.0	40.0	29.0	41.0
38	35.38603449981257	38.0	34.0	40.0	29.0	41.0
39	35.32124989298292	38.0	34.0	40.0	29.0	41.0
40	35.10360084416734	38.0	34.0	40.0	28.0	41.0
41	34.96539046895507	37.0	34.0	40.0	27.0	40.0
42	34.96987064024889	37.0	34.0	40.0	27.0	41.0
43	34.82773949175407	37.0	34.0	40.0	27.0	41.0
44	34.623098732502186	37.0	34.0	40.0	27.0	40.0
45	34.39157029588248	36.0	34.0	40.0	26.0	40.0
46	34.362765869905466	36.0	34.0	39.0	26.0	40.0
47	34.24362573031364	36.0	33.0	39.0	26.0	40.0
48	34.20787246722784	36.0	33.0	39.0	26.0	40.0
49	34.05675936492948	36.0	33.0	39.0	26.0	40.0
50	34.13178935049153	36.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	2.0
13	0.0
14	11.0
15	22.0
16	41.0
17	128.0
18	317.0
19	738.0
20	1333.0
21	2324.0
22	3663.0
23	5907.0
24	9032.0
25	13623.0
26	17305.0
27	19169.0
28	20221.0
29	22975.0
30	28253.0
31	38708.0
32	55708.0
33	106275.0
34	104749.0
35	127142.0
36	163771.0
37	300030.0
38	131120.0
39	30510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.739128519407288	23.36130877523421	30.062863702217395	15.836699003141108
2	30.889575830016152	24.59156879972138	31.377241228547753	13.141614141714717
3	29.015883412477482	23.405778007927992	34.04298470840236	13.535353871192166
4	26.297857414184772	26.41331117906638	32.74298695264401	14.545844454104842
5	17.651958017719537	47.332552558892644	23.908986857887136	11.106502565500687
6	21.577386023694203	39.58734214461395	27.893097627005375	10.942174204686475
7	38.679338597049735	56.47002399676164	3.845466507186976	1.005170899001645
8	87.89173445800319	3.2363626993738563	7.394360636333939	1.47754220628903
9	83.55120486684582	5.021615371891621	8.794767425913012	2.632412335349549
10	54.53731633583497	21.950595098077518	13.581069904802595	9.93101866128492
11	48.09127247670352	18.000064833647667	20.518270205032255	13.390392484616553
12	42.26006770960178	19.89171118438606	24.510942340444807	13.337278765567348
13	21.03427954440232	38.70294469440494	25.173658587673792	15.089117173518945
14	15.389679314492232	39.804784224477366	29.239808857107473	15.565727603922936
15	14.456989108778393	21.571484499355403	48.90618155582468	15.065344836041522
16	16.49376308621462	16.6780402616952	46.69518793030217	20.133008721788013
17	17.24998940219221	16.876780327808895	30.28146946293635	35.59176080706254
18	22.47499956361968	21.163448119367057	34.40006849093035	21.961483826082908
19	29.257264069940543	22.582473802634738	25.935121467501304	22.22514065992341
20	32.12033457486998	21.35312809881978	25.8020462496644	20.72449107664584
21	24.064255132040373	25.633811245978027	27.805156602351133	22.496777019630464
22	24.63761731357625	24.27646064805387	25.939859310984563	25.146062727385317
23	20.76779662848408	28.514752564046088	26.244411214891127	24.473039592578708
24	21.754681114041556	21.735231019741846	38.62946656038381	17.880621305832783
25	18.742410099419903	24.041729595479598	35.288123223828194	21.9277370812723
26	18.599111114066492	31.528270379584384	28.254254292527758	21.61836421382137
27	19.701116884261133	30.453029269067116	30.322364533002403	19.523489313669344
28	17.144925644949335	26.338669364189716	38.808174691770034	17.708230299090914
29	17.251153083047747	23.701519185356908	37.04179027312421	22.00553745847114
30	19.674601584767085	28.096824896785666	31.428858786496978	20.79971473195027
31	28.90026340747366	25.872448941424466	25.249297843283774	19.977989807818105
32	30.76713998000131	25.248716002856003	26.050408992260692	17.93373502488199
33	28.21344234252281	26.741053580022594	25.158447616490687	19.887056460963908
34	20.64045669486376	26.86623239205406	29.50670737333126	22.98660353975092
35	22.65703249745029	24.305968269747872	30.10982653674447	22.927172696057365
36	29.576278864480223	25.20848589327883	25.128856874735572	20.086378367505375
37	22.543906094279762	30.595247693626103	27.7585262480685	19.10231996402564
38	22.25764060381737	31.01026615874768	23.909069977948246	22.8230232594867
39	22.527282082057788	30.14831112503834	26.45187888742136	20.872527905482517
40	24.544107244827646	25.733472199248762	24.7753472548353	24.94707330108829
41	18.007213158903117	24.303308427792356	27.56892938867689	30.120549024627643
42	22.758688332187663	25.66331886767203	24.072733378273583	27.50525942186673
43	23.459141087160525	26.30683438078464	25.849341564435917	24.384682967618918
44	21.261363551354485	29.537295555819693	26.936884443997442	22.264456448828383
45	18.10612603162386	34.68101429748171	23.396634801205906	23.816224869688522
46	22.544820414951968	31.8451240525352	25.68468072337727	19.92537480913556
47	23.288080001396416	25.67537127653296	28.007470831092558	23.029077890978066
48	23.87507387295431	22.33968010413281	30.70845721685775	23.07678880605513
49	21.50465597022307	23.65405763046317	31.35878857498136	23.482497824332402
50	19.69064375656129	31.37358394585892	27.28765110188109	21.648121195698703
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	316.0
1	913.5
2	1511.0
3	6882.0
4	12253.0
5	9352.0
6	6451.0
7	6497.5
8	6544.0
9	6772.0
10	7000.0
11	7012.0
12	7024.0
13	6629.5
14	6235.0
15	5801.5
16	5368.0
17	5172.0
18	4976.0
19	4623.5
20	4271.0
21	4190.5
22	4110.0
23	4895.5
24	5681.0
25	6966.5
26	8252.0
27	9687.0
28	11122.0
29	14014.0
30	16906.0
31	19904.0
32	22902.0
33	25171.5
34	27441.0
35	29850.5
36	32260.0
37	38273.0
38	44286.0
39	58662.5
40	73039.0
41	89765.0
42	106491.0
43	107749.0
44	109007.0
45	103318.5
46	97630.0
47	91747.0
48	85864.0
49	85712.5
50	85561.0
51	82816.0
52	80071.0
53	72548.0
54	65025.0
55	61295.0
56	57565.0
57	55481.0
58	53397.0
59	49671.0
60	45945.0
61	40862.0
62	35779.0
63	31042.0
64	26305.0
65	22138.5
66	17972.0
67	14472.5
68	10973.0
69	8846.5
70	6720.0
71	5543.0
72	4366.0
73	3665.0
74	2964.0
75	2437.0
76	1910.0
77	1319.0
78	728.0
79	550.0
80	372.0
81	325.0
82	278.0
83	211.0
84	144.0
85	94.5
86	45.0
87	29.0
88	13.0
89	6.5
90	0.0
91	1.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1203079.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.322705782125574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.63902697960376	12.75627198449683
2	5.407881865863528	1.8735829293369235
3	2.123441673891211	1.1035126608696504
4	1.3276355879779675	0.919929627056865
5	1.048026492106057	0.9077327287313187
6	0.8634642707103889	0.8974522508936217
7	0.7659495109032882	0.9287822614938454
8	0.6599964072198045	0.914633886362289
9	0.6796173831710244	1.0595530775780782
>10	10.916460329964513	45.01215016737533
>50	2.3416477125448503	27.62078571468824
>100	0.22336941213508898	5.009057940760748
>500	0.001492445960813512	0.1544297949220346
>1k	0.0019899279477513495	0.8421249754342198
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3665	0.3046350239676696	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3088	0.25667474870727525	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1573	0.13074785612582382	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1446	0.12019160836487046	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005070323727702004	0.0
2	0.0	0.0	0.0	0.019948814666368542	0.0
3	0.0	0.0	0.0	0.0294245016328936	0.0
4	0.0	0.0	0.0	0.04313931171602197	0.0
5	0.0	0.0	0.0	0.06782596986565305	0.0
6	0.0	0.0	0.0	0.10980160072613686	0.0
7	0.0	0.0	0.0	0.13673250052573438	0.0
8	0.0	0.0	0.0	0.3111183887342394	0.0
9	0.0	0.0	0.0	0.3855108434275721	0.0
10	0.0	0.0	0.0	0.5097753347868261	0.0
11	0.0	0.0	0.0	0.633541105779421	0.0
12	0.0	0.0	0.0	0.7328695788057144	0.0
13	0.0	0.0	0.0	0.7739308889939895	0.0
14	0.0	0.0	0.0	0.789557460482645	0.0
15	0.0	0.0	0.0	0.8144103587544957	0.0
16	0.0	0.0	0.0	0.8778309653813258	0.0
17	0.0	0.0	0.0	0.9470699762858465	0.0
18	0.0	0.0	0.0	1.0542948551175775	0.0
19	0.0	0.0	0.0	1.1038344115390593	0.0
20	0.0	0.0	0.0	1.1588598919937927	0.0
21	0.0	0.0	0.0	1.2275170624705443	0.0
22	0.0	0.0	0.0	1.2963404730695158	0.0
23	0.0	0.0	0.0	1.3788786937516155	0.0
24	0.0	0.0	0.0	1.441468099767347	0.0
25	0.0	0.0	0.0	1.4975741410165084	0.0
26	0.0	0.0	0.0	1.54736305762132	0.0
27	0.0	0.0	0.0	1.5919154103762096	0.0
28	8.312006110986893E-5	0.0	0.0	1.639293845208835	0.0
29	8.312006110986893E-5	0.0	0.0	1.696979167619084	0.0
30	8.312006110986893E-5	0.0	0.0	1.767381859379143	0.0
31	8.312006110986893E-5	0.0	0.0	1.8407768733391572	0.0
32	8.312006110986893E-5	0.0	0.0	1.9079378827159315	0.0
33	8.312006110986893E-5	0.0	0.0	1.9752651322149253	0.0
34	8.312006110986893E-5	0.0	0.0	2.0499900671526974	0.0
35	8.312006110986893E-5	0.0	0.0	2.1399259732735754	0.0
36	8.312006110986893E-5	0.0	0.0	2.220718672672368	0.0
37	8.312006110986893E-5	0.0	0.0	2.2989346501767547	0.0
38	8.312006110986893E-5	0.0	0.0	2.3769843875589216	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTA	20	7.856628E-4	44.0	1
ATCTCGG	20	7.856628E-4	44.0	18
ATCTCGC	30	2.527815E-6	44.0	17
CGTATTG	20	7.856628E-4	44.0	18
TCCGCAT	20	7.856628E-4	44.0	41
ACGTTAG	55	1.8189894E-12	44.0	2
GCGAAAC	20	7.856628E-4	44.0	25
CAGTTCG	20	7.856628E-4	44.0	35
CGTGAAT	20	7.856628E-4	44.0	33
CGTTGAC	20	7.856628E-4	44.0	28
CGACCCA	30	2.527815E-6	44.0	10
AAGCGTA	20	7.856628E-4	44.0	15
CGACAGT	25	4.4429646E-5	44.0	16
CTACGTC	25	4.4429646E-5	44.0	29
TACGACT	25	4.4429646E-5	44.0	41
GCTTATA	30	2.527815E-6	44.0	1
TCGCTAA	25	4.4429646E-5	44.0	1
GGTATCG	25	4.4429646E-5	44.0	24
ACTCGTT	30	2.527815E-6	44.0	23
TCGTATA	35	1.4461511E-7	44.0	1
>>END_MODULE