FastQCFastQC Report
Thu 26 May 2016
SRR1546390_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546390_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences453685
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11310.24929190958484412No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9830.21667015660645603No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8430.18581174162689973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG406.7993824E-945.0000045
TCACCGG207.028766E-445.00000442
CCGATTA207.028766E-445.00000412
ACTCTAT207.028766E-445.00000411
GCAAACG207.028766E-445.0000041
GCGATAT406.7993824E-945.0000049
CCGGTTG207.028766E-445.00000420
GTGCGTA207.028766E-445.00000419
CATCACG207.028766E-445.00000440
CGCATTA253.8870603E-545.031
GCGTTAG253.8870603E-545.02
TATACGG302.1625856E-644.9999962
CGTTTTA7800.044.711541
AATCGTT1150.043.04347622
GGCGATA1700.042.3529438
TTAGGCG700.041.7857135
TAGGCGA1350.041.6666646
CGTTTTT9150.041.3114781
GTATTAG603.6379788E-1241.2499962
ATAGGCC603.6379788E-1241.2499965