##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546390_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	453685
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.061959288933952	31.0	31.0	33.0	30.0	33.0
2	31.21188048976713	31.0	31.0	33.0	30.0	34.0
3	31.19147426077565	31.0	31.0	34.0	28.0	34.0
4	35.14085103100169	35.0	35.0	37.0	33.0	37.0
5	32.92290245434608	35.0	35.0	37.0	28.0	37.0
6	34.08033327088178	35.0	35.0	37.0	29.0	37.0
7	14.964184401071227	0.0	0.0	35.0	0.0	37.0
8	24.733169489844276	17.0	17.0	35.0	17.0	37.0
9	32.67354882793127	32.0	32.0	37.0	27.0	39.0
10	35.565844142962625	37.0	35.0	37.0	32.0	39.0
11	36.531935153245094	37.0	35.0	39.0	33.0	39.0
12	36.32629026747633	37.0	35.0	39.0	32.0	39.0
13	35.92485975952478	37.0	35.0	39.0	31.0	39.0
14	36.98274353350893	39.0	35.0	40.0	31.0	41.0
15	37.34072539316927	39.0	35.0	41.0	32.0	41.0
16	37.43239913155603	39.0	35.0	41.0	32.0	41.0
17	37.412643133451624	39.0	35.0	41.0	32.0	41.0
18	37.31226952621312	39.0	35.0	41.0	32.0	41.0
19	37.23212360999372	39.0	35.0	40.0	32.0	41.0
20	37.343070632707715	39.0	35.0	41.0	32.0	41.0
21	37.20430254471715	39.0	35.0	40.0	32.0	41.0
22	37.24952775604219	39.0	35.0	41.0	32.0	41.0
23	37.220886738596164	39.0	35.0	41.0	32.0	41.0
24	37.09246724048624	39.0	35.0	41.0	32.0	41.0
25	36.830891477567036	38.0	35.0	40.0	31.0	41.0
26	36.694741946504735	38.0	35.0	40.0	31.0	41.0
27	36.61363941942096	38.0	35.0	40.0	31.0	41.0
28	36.5470227139976	38.0	35.0	40.0	31.0	41.0
29	36.58785721370555	38.0	35.0	40.0	31.0	41.0
30	36.35337734331089	38.0	35.0	40.0	31.0	41.0
31	36.21412433737064	38.0	35.0	40.0	30.0	41.0
32	36.1062565436371	38.0	35.0	40.0	30.0	41.0
33	35.92146092553203	38.0	35.0	40.0	30.0	41.0
34	35.7826399374015	38.0	35.0	40.0	29.0	41.0
35	35.630272105094946	38.0	34.0	40.0	29.0	41.0
36	35.38636719309653	38.0	34.0	40.0	27.0	41.0
37	35.22497107023596	38.0	34.0	40.0	27.0	41.0
38	35.17462556619681	38.0	34.0	40.0	27.0	41.0
39	35.06151404608925	38.0	34.0	40.0	26.0	41.0
40	34.918002578881826	38.0	34.0	40.0	25.0	41.0
41	34.80786889581979	37.0	34.0	40.0	25.0	41.0
42	34.78023959355059	37.0	34.0	40.0	25.0	41.0
43	34.680685938481545	37.0	33.0	40.0	24.0	41.0
44	34.48425449375668	37.0	33.0	40.0	24.0	41.0
45	34.3987921134708	37.0	33.0	40.0	24.0	41.0
46	34.38480002645007	37.0	33.0	40.0	24.0	41.0
47	34.33428480112854	37.0	33.0	40.0	24.0	41.0
48	34.3053638537752	36.0	33.0	40.0	24.0	41.0
49	34.21790449320564	36.0	33.0	40.0	24.0	41.0
50	34.08122816491618	36.0	33.0	40.0	24.0	41.0
51	33.90931813923758	36.0	33.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	5.0
15	15.0
16	42.0
17	100.0
18	252.0
19	488.0
20	753.0
21	1291.0
22	2088.0
23	3017.0
24	4299.0
25	6060.0
26	7346.0
27	8152.0
28	8861.0
29	10417.0
30	12757.0
31	17034.0
32	22848.0
33	35509.0
34	44859.0
35	46545.0
36	54023.0
37	81560.0
38	71888.0
39	13474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.185040281252412	24.151779318249446	30.242789600714154	15.420390799783993
2	30.448659312077766	24.526047808501495	31.871452659885165	13.153840219535581
3	29.727013236055853	22.908626029073034	33.718328796411605	13.646031938459505
4	26.703329402559046	27.029987766842634	31.494539162634865	14.772143667963455
5	21.478558912020453	35.80303514553049	28.941886992076	13.776518950373056
6	21.70051908262341	40.183387151878506	27.422550888832557	10.693542876665528
7	37.28512073354861	57.93953954836506	4.001454753849036	0.7738849642373012
8	88.30950990224495	3.495376748184313	6.76967499476509	1.425438354805647
9	83.81255717072418	5.254306402019022	8.229939275047665	2.703197152209132
10	54.805426672691404	22.284624794736434	13.087935461829241	9.822013070742916
11	48.65336081201715	18.19720731344435	19.928584811047312	13.22084706349119
12	42.706062576457235	20.246646902586598	23.90028323616606	13.147007284790108
13	21.134046750498694	39.53668294080695	24.336488973627077	14.992781335067281
14	15.353383955828384	40.685277229796	28.648732049770214	15.3126067646054
15	14.344975037746455	21.964578947948464	48.67231669550459	15.018129318800488
16	16.436293904360955	17.146257866140605	46.42295866074479	19.99448956875365
17	17.071095583940398	17.14317202464265	30.026560278607406	35.75917211280955
18	22.198221232793678	21.36989320784245	34.44173821043235	21.990147348931526
19	29.74332411254505	22.702095065959863	25.504700397853135	22.049880423641955
20	32.657019738364724	21.583477522950947	25.32395825297288	20.43554448571145
21	23.97125759061904	26.348237213044296	27.38397787010812	22.296527326228553
22	24.51150027001113	24.63383184368009	25.237113856530414	25.617554029778372
23	20.69894309928695	28.91808192909177	25.767437759679073	24.615537211942208
24	21.2676196039102	22.02585494340787	38.95610390469158	17.750421547990346
25	18.51945733273086	24.426860046067205	35.364625235570934	21.689057385631
26	18.35921399208702	32.327495949833036	27.8825616892778	21.430728368802143
27	19.468794427851925	31.550965978597485	29.77109668602665	19.209142907523944
28	16.974332411254505	26.985243065122276	38.532461950472246	17.507962573150976
29	17.049274276204855	24.182417315979148	36.737383867661485	22.030924540154512
30	19.594432260268686	28.98795419729548	30.91285804027023	20.5047555021656
31	29.355830587301764	26.286740800335036	24.738309620110872	19.619118992252332
32	31.319087031751103	25.33960787771251	25.69734507422551	17.643960016310878
33	28.9390215678279	26.81794637248311	24.63779935417746	19.605232705511533
34	20.634801679579443	26.951298808644765	29.011098008530144	23.402801503245644
35	22.70363798670884	24.27631506441694	30.19055071249876	22.82949623637546
36	29.956688010403692	25.542391747578165	24.751755072351962	19.749165169666178
37	22.467350694865377	31.4742607756483	27.44040468607073	18.617983843415587
38	22.032026626403784	31.746255661968103	23.560399836891236	22.661317874736877
39	22.596294786029954	30.780387273107994	26.242436933114387	20.380881007747668
40	24.853808259034352	25.810859957900306	24.524504887752517	24.810826895312825
41	17.964005863098844	24.401291645084143	27.332841068141995	30.30186142367502
42	22.493580347598	25.52277461234116	24.00432017809714	27.979324861963693
43	23.126177854678907	26.149641270925862	25.849432976624755	24.87474789777048
44	20.859627274430498	29.626723387372294	27.282365517925435	22.231283820271774
45	18.002578881823293	35.479462622744855	23.118683668183873	23.399274827247982
46	22.34149244519876	32.522565215953804	25.3032390314866	19.832703307360834
47	22.98378831127324	26.109745748702295	27.681761574660836	23.224704365363632
48	23.49295215843592	22.658011615989068	31.06031718042254	22.788719045152472
49	21.454753849036226	24.048403628067934	31.681893825010743	22.814948697885097
50	19.62507025799839	32.3089809008453	26.940718780651775	21.125230060504535
51	19.281880599975754	32.5532032136835	24.491883134774127	23.673033051566616
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	138.0
1	361.5
2	585.0
3	2230.0
4	3875.0
5	2956.5
6	2038.0
7	2138.5
8	2239.0
9	2238.5
10	2238.0
11	2217.0
12	2196.0
13	2101.0
14	2006.0
15	1945.0
16	1884.0
17	1782.0
18	1680.0
19	1640.0
20	1600.0
21	1496.0
22	1392.0
23	1661.5
24	1931.0
25	2410.0
26	3256.0
27	3623.0
28	4575.5
29	5528.0
30	6944.5
31	8361.0
32	9213.0
33	10065.0
34	10957.5
35	11850.0
36	13901.5
37	15953.0
38	20133.0
39	24313.0
40	30734.5
41	37156.0
42	40063.5
43	42971.0
44	39642.5
45	36314.0
46	34719.5
47	33125.0
48	32364.0
49	31603.0
50	30655.0
51	29707.0
52	27657.5
53	25608.0
54	24246.5
55	22885.0
56	21910.5
57	20936.0
58	19636.5
59	18337.0
60	16826.0
61	15315.0
62	13165.5
63	11016.0
64	9432.0
65	7848.0
66	6615.0
67	5382.0
68	4392.0
69	3402.0
70	2731.5
71	2061.0
72	1806.0
73	1551.0
74	1280.0
75	775.0
76	541.0
77	389.0
78	237.0
79	189.0
80	141.0
81	119.0
82	97.0
83	63.5
84	30.0
85	21.5
86	13.0
87	10.0
88	7.0
89	5.0
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	453685.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.567591033363794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.04108957841588	16.716056622269928
2	6.261403347364926	3.076551934659932
3	3.4644712460437654	2.5534113815895454
4	2.5682829444639603	2.523861001502158
5	2.0185894657546792	2.4795940229458635
6	1.5443793024996184	2.2765007462521356
7	1.387235326879877	2.385672112447369
8	1.2067584614013	2.3717718684606774
9	1.0891352146601359	2.408168568042459
>10	12.21483509160084	59.18310638833795
>50	0.18945385203996923	2.9186110879029203
>100	0.011672512210993366	0.4544130331101214
>500	0.0017957711093835945	0.40279524197042793
>1k	8.978855546917972E-4	0.2494859905085181
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1131	0.24929190958484412	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	983	0.21667015660645603	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	843	0.18581174162689973	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005730848496203313	0.0
2	0.0	0.0	0.0	0.021380473235835435	0.0
3	0.0	0.0	0.0	0.024466314733791066	0.0
4	0.0	0.0	0.0	0.034825925476927824	0.0
5	0.0	0.0	0.0	0.0608351609596967	0.0
6	0.0	0.0	0.0	0.08816689994158942	0.0
7	0.0	0.0	0.0	0.11042904217684076	0.0
8	0.0	0.0	0.0	0.25127566483352987	0.0
9	0.0	0.0	0.0	0.31144957404366463	0.0
10	0.0	0.0	0.0	0.4093148329788289	0.0
11	0.0	0.0	0.0	0.5071800919139932	0.0
12	0.0	0.0	0.0	0.592040733107773	0.0
13	0.0	0.0	0.0	0.6270870758345548	0.0
14	0.0	0.0	0.0	0.6374466865776915	0.0
15	0.0	0.0	0.0	0.659047577063381	0.0
16	0.0	0.0	0.0	0.7064372857819853	0.0
17	0.0	0.0	0.0	0.767052029491828	0.0
18	0.0	0.0	0.0	0.8616110296791827	0.0
19	0.0	0.0	0.0	0.9015065519027519	0.0
20	0.0	0.0	0.0	0.9427245776254449	0.0
21	0.0	0.0	0.0	0.9971676383393765	0.0
22	0.0	0.0	0.0	1.0549169578011175	0.0
23	0.0	0.0	0.0	1.1291975710019067	0.0
24	0.0	0.0	0.0	1.1820977109668602	0.0
25	0.0	0.0	0.0	1.2308099231845884	0.0
26	0.0	0.0	0.0	1.2746729559055292	0.0
27	0.0	0.0	0.0	1.3161113988780762	0.0
28	0.0	0.0	0.0	1.3564477556013534	0.0
29	0.0	0.0	0.0	1.4073641403176214	0.0
30	0.0	0.0	0.0	1.4609255320321368	0.0
31	0.0	0.0	0.0	1.5248465344897892	0.0
32	0.0	0.0	0.0	1.5803916814529906	0.0
33	0.0	0.0	0.0	1.6313080661692585	0.0
34	0.0	0.0	0.0	1.6941269823776408	0.0
35	0.0	0.0	0.0	1.7800897098206905	0.0
36	0.0	0.0	0.0	1.8545907402713335	0.0
37	0.0	0.0	0.0	1.9323980294697862	0.0
38	0.0	0.0	0.0	2.0064582254207215	0.0
39	0.0	0.0	0.0	2.093082204613333	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	40	6.7993824E-9	45.000004	5
TCACCGG	20	7.028766E-4	45.000004	42
CCGATTA	20	7.028766E-4	45.000004	12
ACTCTAT	20	7.028766E-4	45.000004	11
GCAAACG	20	7.028766E-4	45.000004	1
GCGATAT	40	6.7993824E-9	45.000004	9
CCGGTTG	20	7.028766E-4	45.000004	20
GTGCGTA	20	7.028766E-4	45.000004	19
CATCACG	20	7.028766E-4	45.000004	40
CGCATTA	25	3.8870603E-5	45.0	31
GCGTTAG	25	3.8870603E-5	45.0	2
TATACGG	30	2.1625856E-6	44.999996	2
CGTTTTA	780	0.0	44.71154	1
AATCGTT	115	0.0	43.043476	22
GGCGATA	170	0.0	42.352943	8
TTAGGCG	70	0.0	41.785713	5
TAGGCGA	135	0.0	41.666664	6
CGTTTTT	915	0.0	41.311478	1
GTATTAG	60	3.6379788E-12	41.249996	2
ATAGGCC	60	3.6379788E-12	41.249996	5
>>END_MODULE