FastQCFastQC Report
Thu 26 May 2016
SRR1546389_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546389_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences652092
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26580.40761119596621337No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.2902964612355312No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8220.12605583261257614No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7820.11992172883580844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAC207.853972E-444.00000424
AACGTAG207.853972E-444.0000042
CTATGCG207.853972E-444.00000433
TTAGGCC800.044.0000045
ACGCCGG207.853972E-444.00000427
TCACCCG207.853972E-444.00000412
ATCTAGT207.853972E-444.00000420
CGCATGA207.853972E-444.00000417
CGTGAAT207.853972E-444.00000439
ATTACGG408.303687E-944.0000042
TGGAACG207.853972E-444.0000041
TAGCGAA207.853972E-444.0000041
TCTTACG207.853972E-444.0000041
CAATTAG207.853972E-444.0000042
ATCGTAC207.853972E-444.00000411
ATACTCC207.853972E-444.00000427
TGCGCAA408.303687E-944.0000041
TTGACAC207.853972E-444.00000441
ACTACCT207.853972E-444.00000438
ATGACCG207.853972E-444.00000437
Produced by FastQC (version 0.11.5)