##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546389_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	652092
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62737006434675	31.0	31.0	33.0	30.0	34.0
2	31.811661544690015	31.0	31.0	34.0	30.0	34.0
3	31.847105929838122	31.0	31.0	34.0	30.0	34.0
4	35.7168390349828	37.0	35.0	37.0	35.0	37.0
5	27.409689123620595	35.0	28.0	37.0	0.0	37.0
6	31.509963318059416	36.0	30.0	37.0	17.0	37.0
7	14.652420210645124	0.0	0.0	32.0	0.0	37.0
8	24.940313636726106	17.0	17.0	35.0	17.0	37.0
9	33.302080074590705	32.0	32.0	37.0	28.0	39.0
10	36.24225876103372	37.0	35.0	39.0	33.0	39.0
11	37.197288112720294	39.0	37.0	39.0	34.0	39.0
12	36.863983916379894	39.0	35.0	39.0	33.0	39.0
13	36.14981321654	38.0	35.0	39.0	32.0	39.0
14	37.41917091453353	40.0	35.0	41.0	33.0	41.0
15	37.81519325493949	40.0	35.0	41.0	33.0	41.0
16	37.947074952614045	40.0	35.0	41.0	33.0	41.0
17	38.00862915048797	40.0	35.0	41.0	34.0	41.0
18	37.96816553492452	40.0	36.0	41.0	34.0	41.0
19	37.88105666071659	40.0	36.0	41.0	34.0	41.0
20	37.890188807714246	40.0	35.0	41.0	34.0	41.0
21	37.72523815657913	40.0	35.0	41.0	34.0	41.0
22	37.634642044374104	39.0	35.0	41.0	33.0	41.0
23	37.53892242198953	39.0	35.0	41.0	33.0	41.0
24	37.414177754059246	39.0	35.0	41.0	33.0	41.0
25	37.24331536040927	39.0	35.0	41.0	33.0	41.0
26	36.982071548186454	38.0	35.0	40.0	33.0	41.0
27	36.77095716555333	38.0	35.0	40.0	32.0	41.0
28	36.836561405445856	38.0	35.0	40.0	33.0	41.0
29	36.89002011986039	38.0	35.0	40.0	33.0	41.0
30	36.71880501524325	38.0	35.0	40.0	32.0	41.0
31	36.44961753862952	38.0	35.0	40.0	32.0	41.0
32	36.1409678388939	38.0	35.0	40.0	31.0	41.0
33	35.94264919673911	38.0	35.0	40.0	31.0	41.0
34	35.73837433981708	38.0	35.0	40.0	30.0	41.0
35	35.610447605552594	38.0	35.0	40.0	30.0	41.0
36	35.42261828085608	38.0	35.0	40.0	29.0	41.0
37	35.17725412978537	38.0	34.0	40.0	29.0	41.0
38	35.05392337277562	37.0	34.0	40.0	28.0	41.0
39	35.02796844617017	37.0	34.0	40.0	28.0	41.0
40	34.77771694791532	37.0	34.0	40.0	27.0	40.0
41	34.6981560884047	37.0	34.0	40.0	26.0	40.0
42	34.68567472074493	37.0	34.0	40.0	26.0	41.0
43	34.54412567551818	36.0	33.0	40.0	26.0	41.0
44	34.35481343123363	36.0	33.0	40.0	26.0	40.0
45	34.09024646828975	36.0	33.0	39.0	24.0	40.0
46	34.11130024597756	36.0	33.0	39.0	25.0	40.0
47	33.98112996325672	36.0	33.0	39.0	24.0	40.0
48	34.024447470602304	36.0	33.0	39.0	25.0	40.0
49	33.93839519576992	36.0	33.0	39.0	24.0	40.0
50	33.95037663397189	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	4.0
15	9.0
16	27.0
17	81.0
18	175.0
19	399.0
20	802.0
21	1357.0
22	2253.0
23	3619.0
24	5575.0
25	8329.0
26	10316.0
27	11038.0
28	11384.0
29	12926.0
30	15919.0
31	22154.0
32	31870.0
33	66505.0
34	60526.0
35	69321.0
36	79660.0
37	153399.0
38	70383.0
39	14058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.939180361053346	23.278003717266888	30.405525600682115	16.377290320997652
2	30.645829116136987	24.37140771547573	31.21292701029916	13.769836158088122
3	28.219484367236525	22.998288585046282	35.58362930384057	13.19859774387663
4	24.951540580163538	27.759579936573363	32.45968360292719	14.829195880335904
5	17.68216754691056	47.439931788766	24.480594762702196	10.397305901621245
6	21.90304435570441	38.27926120854113	28.681995792004813	11.135698643749654
7	38.058740177766325	57.05513945884937	3.905737227262411	0.9803831361218969
8	88.641019978776	2.6083742784760435	7.494034584077093	1.2565711586708626
9	85.58807652907872	3.6200413438594556	8.780662851254117	2.0112192758077083
10	61.49623059322917	17.50780564705594	12.12788992964183	8.868073830073058
11	57.37733325972408	14.468050520478707	17.85116210596051	10.303454113836699
12	48.689295375499164	19.700441042061552	20.87374174196279	10.736521840476497
13	19.694153585690362	47.304521447893855	22.46140115198469	10.539923814431093
14	12.16408114192476	48.688528612527065	28.1944878943462	10.952902351201978
15	10.99047373683468	19.915441379437258	57.123841421149166	11.9702434625789
16	11.83744011581188	13.831483901044638	56.228415622335504	18.102660360807985
17	13.394582359544357	13.679051422191959	30.5180864049858	42.408279813277886
18	21.57425639326966	20.03996368610564	36.35008557074769	22.035694349877012
19	30.01769688939597	21.50432761021451	27.593345724222964	20.884629776166552
20	34.13659422290106	19.92832299736847	24.26160725787159	21.67347552185888
21	21.74524453604706	26.882709801684424	29.499518472853524	21.87252718941499
22	22.874072983566737	23.617219656122142	25.877636897861038	27.631070462450086
23	18.769130736153794	31.052673549131104	24.08126460683462	26.096931107880483
24	20.096704146040743	21.479944547701855	42.17211681787232	16.251234488385073
25	14.981935064377419	22.769639866767267	40.59795243615931	21.650472632696
26	14.754819872042596	36.04675413898653	29.906976316225318	19.291449672745564
27	16.81618544622538	33.68343730639235	30.930911589162264	18.569465658220004
28	13.285855370101151	27.150156726351497	43.62589941296627	15.938088490581084
29	14.029768805628654	22.04550891591984	43.649209007317985	20.275513271133523
30	18.043466259362177	28.297694190390317	34.196401734724546	19.46243781552296
31	31.352784576409466	24.2824632107126	25.38844212166381	18.976310091214124
32	32.43729412414199	25.99525833778056	25.895119093624825	15.672328444452624
33	30.362433521650317	25.23416941167811	25.82641713132503	18.576979935346547
34	19.915901437220516	26.42418554437104	29.366408420897667	24.293504597510783
35	19.597541451206272	24.88360538083583	32.649687467412576	22.86916570054532
36	32.881403237579974	21.76840077780436	27.0047784668421	18.345417517773566
37	21.47764425878557	30.43987658183201	29.249400391355824	18.8330787680266
38	21.846763953552568	32.79092520687265	22.933113732418125	22.42919710715666
39	20.452942222876526	32.48222643430682	26.438447335652025	20.62638400716463
40	24.64146163424793	25.283855652269928	24.095526398115602	25.97915631536654
41	16.85053642737528	23.012857081516106	26.713868595228895	33.42273789587972
42	23.54943780938886	23.92147120344982	23.828999589015044	28.70009139814627
43	23.55679873392098	26.39596866699791	24.40008465063212	25.64714794844899
44	20.130441716812967	31.992571600326336	27.382332554302153	20.494654128558548
45	16.832747526422654	38.98897701551315	21.098709997975746	23.079565460088453
46	22.773780386816583	32.81193451230808	25.811081871883108	18.60320322899223
47	23.7007968200806	26.389067800249045	26.80726032523018	23.102875054440172
48	25.85724100280328	21.0615986701263	30.61439183428105	22.46676849278936
49	20.809487004901147	21.0655858375812	34.09840942689068	24.026517730626967
50	18.32900879017071	34.87422020205738	25.685946154837048	21.11082485293486
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	263.0
1	498.5
2	734.0
3	3345.0
4	5956.0
5	4614.5
6	3273.0
7	3591.0
8	3909.0
9	4212.5
10	4516.0
11	4649.5
12	4783.0
13	4416.0
14	4049.0
15	3782.0
16	3515.0
17	3167.5
18	2820.0
19	2597.0
20	2374.0
21	2365.5
22	2357.0
23	2554.5
24	2752.0
25	3245.0
26	3738.0
27	4386.0
28	5034.0
29	5787.0
30	6540.0
31	7359.0
32	8178.0
33	9499.0
34	10820.0
35	11959.5
36	13099.0
37	17541.5
38	21984.0
39	34369.5
40	46755.0
41	59184.5
42	71614.0
43	71633.0
44	71652.0
45	68991.0
46	66330.0
47	60490.5
48	54651.0
49	52057.5
50	49464.0
51	46679.0
52	43894.0
53	38843.0
54	33792.0
55	29489.0
56	25186.0
57	23209.5
58	21233.0
59	19468.0
60	17703.0
61	15573.5
62	13444.0
63	11310.5
64	9177.0
65	7565.0
66	5953.0
67	5061.0
68	4169.0
69	3347.5
70	2526.0
71	2109.0
72	1692.0
73	1315.5
74	939.0
75	779.0
76	619.0
77	502.5
78	386.0
79	273.5
80	161.0
81	95.0
82	29.0
83	19.0
84	9.0
85	8.0
86	7.0
87	7.0
88	7.0
89	5.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	652092.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.002090475915487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.23414614059902	14.84838107506594
2	5.283509003577069	2.1136245023972524
3	2.064830550041514	1.2390278243809412
4	1.3980712551787555	1.1185739095144878
5	1.068032104241467	1.0681437390110111
6	0.8860871236900782	1.06341568905556
7	0.80527098840279	1.1274972217363746
8	0.6938820878913811	1.1103273841296435
9	0.720894239593737	1.2977452623528203
>10	11.180992937269597	51.95071299291987
>50	1.5808375966698474	20.07224504334549
>100	0.08035538125790037	2.0390763795679083
>500	0.001545295793421161	0.24789135310193458
>1k	0.001545295793421161	0.7033376234207632
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2658	0.40761119596621337	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1893	0.2902964612355312	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	822	0.12605583261257614	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	782	0.11992172883580844	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008281040098636389	0.0
2	0.0	0.0	0.0	0.032050692233611205	0.0
3	0.0	0.0	0.0	0.04278537384295467	0.0
4	0.0	0.0	0.0	0.057967280690454724	0.0
5	0.0	0.0	0.0	0.09293167221803059	0.0
6	0.0	0.0	0.0	0.14752519583126308	0.0
7	0.0	0.0	0.0	0.17804236212068236	0.0
8	0.0	0.0	0.0	0.3703465155223496	0.0
9	0.0	0.0	0.0	0.4569907313691933	0.0
10	0.0	0.0	0.0	0.6085030946553553	0.0
11	0.0	0.0	0.0	0.7882323353146489	0.0
12	0.0	0.0	0.0	0.9469522705385129	0.0
13	0.0	0.0	0.0	1.0167277009992455	0.0
14	0.0	0.0	0.0	1.0357434227072253	0.0
15	0.0	0.0	0.0	1.0769952706059882	0.0
16	0.0	0.0	0.0	1.1688534746630843	0.0
17	0.0	0.0	0.0	1.2788072848616452	0.0
18	0.0	0.0	0.0	1.4341534630082873	0.0
19	0.0	0.0	0.0	1.4999417260141208	0.0
20	0.0	0.0	0.0	1.5733976187409138	0.0
21	0.0	0.0	0.0	1.6716966317636162	0.0
22	0.0	0.0	0.0	1.7584942002048791	0.0
23	0.0	0.0	0.0	1.861240438465738	0.0
24	0.0	0.0	0.0	1.9368432675143998	0.0
25	0.0	0.0	0.0	1.994350490421597	0.0
26	0.0	0.0	0.0	2.0595253430497538	0.0
27	0.0	0.0	0.0	2.1191795022788193	0.0
28	0.0	0.0	0.0	2.18174736080185	0.0
29	0.0	0.0	0.0	2.2426283407862693	0.0
30	0.0	0.0	0.0	2.3301926721996282	0.0
31	0.0	0.0	0.0	2.4205173503125326	0.0
32	0.0	0.0	0.0	2.493359832661649	0.0
33	0.0	0.0	0.0	2.558074627506548	0.0
34	0.0	0.0	0.0	2.640118265520816	0.0
35	0.0	0.0	0.0	2.7393373941100334	0.0
36	0.0	0.0	0.0	2.8155536335363722	0.0
37	0.0	0.0	0.0	2.8946835722566755	0.0
38	0.0	0.0	0.0	2.968752875361145	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAC	20	7.853972E-4	44.000004	24
AACGTAG	20	7.853972E-4	44.000004	2
CTATGCG	20	7.853972E-4	44.000004	33
TTAGGCC	80	0.0	44.000004	5
ACGCCGG	20	7.853972E-4	44.000004	27
TCACCCG	20	7.853972E-4	44.000004	12
ATCTAGT	20	7.853972E-4	44.000004	20
CGCATGA	20	7.853972E-4	44.000004	17
CGTGAAT	20	7.853972E-4	44.000004	39
ATTACGG	40	8.303687E-9	44.000004	2
TGGAACG	20	7.853972E-4	44.000004	1
TAGCGAA	20	7.853972E-4	44.000004	1
TCTTACG	20	7.853972E-4	44.000004	1
CAATTAG	20	7.853972E-4	44.000004	2
ATCGTAC	20	7.853972E-4	44.000004	11
ATACTCC	20	7.853972E-4	44.000004	27
TGCGCAA	40	8.303687E-9	44.000004	1
TTGACAC	20	7.853972E-4	44.000004	41
ACTACCT	20	7.853972E-4	44.000004	38
ATGACCG	20	7.853972E-4	44.000004	37
>>END_MODULE