FastQCFastQC Report
Thu 26 May 2016
SRR1546388_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546388_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences246498
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10410.422315799722513No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.21095505845889215No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.17606633725222923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCT351.2067358E-745.00000411
ATAGGCG351.2067358E-745.0000045
GTTTAAG351.2067358E-745.0000042
TGCATAG650.045.0000042
GTACGGG351.2067358E-745.0000043
ATCTGAG351.2067358E-745.0000042
TCGCCGA207.0224935E-445.027
GTGTTAA253.8818605E-545.01
GCGACTG207.0224935E-445.020
TTGTGCA207.0224935E-445.01
GGATGGC502.1827873E-1145.08
ACGCAGA207.0224935E-445.01
CGCATTG207.0224935E-445.012
GACCGTT207.0224935E-445.014
GACCGAT950.045.09
TCCTCGC253.8818605E-545.024
AAGGTAC207.0224935E-445.06
CGATTAT253.8818605E-545.010
CGCGACC253.8818605E-545.010
TATGCAA207.0224935E-445.01