FastQCFastQC Report
Thu 26 May 2016
SRR1546387_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546387_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1560911
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56080.3592773707149223No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA54450.3488347509883651No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24420.1564471004432668No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22420.1436340701039329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG551.8189894E-1244.0000042
TCGTTAA351.4465331E-744.01
ACCGGTT302.5283025E-644.024
GTACCCG207.857349E-444.022
GGTAATC254.4435754E-544.08
CGTATAT302.5283025E-644.042
ACGCACG207.857349E-444.010
CACATCG207.857349E-444.028
GCACGAT302.5283025E-644.09
CACGATC254.4435754E-544.043
CGCCTAT454.802132E-1044.017
ACGATTC408.3164196E-944.09
AATGCGC302.5283025E-644.034
ATATCGA302.5283025E-644.01
CCCGTAC254.4435754E-544.036
CCGTACG254.4435754E-544.042
CGTAGTA207.857349E-444.039
GTTCGAC207.857349E-444.042
TACGATT207.857349E-444.018
CGCTAGC207.857349E-444.044
Produced by FastQC (version 0.11.5)