##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546387_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1560911 Sequences flagged as poor quality 0 Sequence length 50 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58019003005296 31.0 31.0 33.0 30.0 34.0 2 31.765195453168054 31.0 31.0 34.0 30.0 34.0 3 31.78602815919678 31.0 31.0 34.0 30.0 34.0 4 35.66793878702886 37.0 35.0 37.0 35.0 37.0 5 27.182060988743114 35.0 26.0 37.0 0.0 37.0 6 31.378523823587635 35.0 28.0 37.0 17.0 37.0 7 14.919766085318125 0.0 0.0 32.0 0.0 37.0 8 25.0782261128277 17.0 17.0 35.0 17.0 37.0 9 33.268388140002855 32.0 32.0 37.0 28.0 39.0 10 36.19075591113138 37.0 35.0 39.0 33.0 39.0 11 37.116733753558016 39.0 37.0 39.0 34.0 39.0 12 36.86067303004464 39.0 35.0 39.0 33.0 39.0 13 36.17853163953614 38.0 35.0 39.0 32.0 39.0 14 37.42953121606549 40.0 35.0 41.0 33.0 41.0 15 37.80579289914672 40.0 35.0 41.0 33.0 41.0 16 37.92871086179802 40.0 35.0 41.0 33.0 41.0 17 37.97994824817046 40.0 35.0 41.0 34.0 41.0 18 37.955971224496466 40.0 36.0 41.0 34.0 41.0 19 37.877952042108745 40.0 36.0 41.0 34.0 41.0 20 37.87202793753135 40.0 35.0 41.0 34.0 41.0 21 37.69967602252787 40.0 35.0 41.0 33.0 41.0 22 37.603784584771326 39.0 35.0 41.0 33.0 41.0 23 37.522576879783664 39.0 35.0 41.0 33.0 41.0 24 37.382144145310015 39.0 35.0 41.0 33.0 41.0 25 37.21063724965741 39.0 35.0 41.0 33.0 41.0 26 36.92030038868327 38.0 35.0 40.0 33.0 41.0 27 36.70626063881925 38.0 35.0 40.0 32.0 41.0 28 36.74451970676099 38.0 35.0 40.0 32.0 41.0 29 36.77659136235186 38.0 35.0 40.0 32.0 41.0 30 36.61786738641729 38.0 35.0 40.0 32.0 41.0 31 36.36760391848094 38.0 35.0 40.0 31.0 41.0 32 36.08607217195599 38.0 35.0 40.0 31.0 41.0 33 35.8447938415451 38.0 35.0 40.0 30.0 41.0 34 35.65043618758533 38.0 35.0 40.0 30.0 41.0 35 35.49332537217048 38.0 35.0 40.0 29.0 41.0 36 35.27135948173855 38.0 34.0 40.0 28.0 41.0 37 35.057071799737464 38.0 34.0 40.0 27.0 41.0 38 34.92155542500501 37.0 34.0 40.0 27.0 41.0 39 34.902244266329085 37.0 34.0 40.0 27.0 41.0 40 34.67238234595054 37.0 34.0 40.0 26.0 41.0 41 34.53016347504759 37.0 34.0 40.0 25.0 40.0 42 34.53126475500525 37.0 34.0 40.0 25.0 40.0 43 34.38455171371078 37.0 33.0 40.0 24.0 41.0 44 34.1696490062534 36.0 33.0 40.0 24.0 40.0 45 33.942106885017786 36.0 33.0 39.0 23.0 40.0 46 33.92865064055542 36.0 33.0 39.0 23.0 40.0 47 33.79929092690102 36.0 33.0 39.0 23.0 40.0 48 33.78040323887781 35.0 33.0 39.0 23.0 40.0 49 33.65703105430098 35.0 33.0 39.0 24.0 40.0 50 33.69313304858509 35.0 33.0 39.0 24.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 2.0 13 4.0 14 9.0 15 21.0 16 60.0 17 227.0 18 532.0 19 1159.0 20 2242.0 21 3865.0 22 6124.0 23 9661.0 24 14570.0 25 21200.0 26 26592.0 27 28539.0 28 29428.0 29 32325.0 30 39731.0 31 54015.0 32 77126.0 33 152508.0 34 141988.0 35 162970.0 36 189459.0 37 360506.0 38 169598.0 39 36449.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.130424476475596 23.461875789202587 30.790352556936302 16.617347177385515 2 32.18902294877799 25.24551367758956 30.011192182001405 12.554271191631042 3 28.223966645119418 23.317280741823204 35.29362019999859 13.165132413058783 4 25.81011985949231 27.03978638115818 33.05274932395248 14.097344435397021 5 17.452756755510084 47.75301090196686 23.913342913209018 10.880889429314035 6 21.322548178595703 38.86602118890827 28.71758863894226 11.093841993553765 7 38.32953960860036 56.32274998382355 4.326191563772695 1.0215188438033942 8 87.15211821814313 3.057509364723549 8.212447730844358 1.5779246862889684 9 83.3557454589019 4.920588041214393 9.48279562383762 2.240870876046104 10 59.02892605664256 19.37118772306685 13.187363020697529 8.412523199593059 11 54.497790072592224 17.03287375129011 18.57133430413393 9.898001871983734 12 47.91368630242211 19.896393836676147 21.465029075969095 10.724890784932645 13 20.799520280144097 44.59286916422525 22.12919250360847 12.478418052022185 14 13.281218467933147 46.551597112199225 27.516367044629707 12.65081737523792 15 12.416787376089989 21.74448126766997 53.912234586084665 11.926496770155378 16 14.012906565460812 15.41657403913484 52.645154015827934 17.925365379576412 17 14.753755979681097 16.293497835558853 30.62954902617766 38.32319715858239 18 21.772221478354627 22.344515478460973 34.930242659575086 20.953020383609317 19 30.287505181269143 22.830577784383607 26.74111464394831 20.14080239039894 20 33.83844434436044 21.74371248584961 24.75637624438549 19.661466925404458 21 22.3555346845528 27.002564528022415 28.42538748205375 22.21651330537103 22 21.902914387815834 25.98098161906733 25.872711512699954 26.243392480416883 23 20.462473517067917 29.334151658871004 24.90103535691657 25.302339467144506 24 20.26521691499387 22.553431938143813 40.078646380222835 17.10270476663948 25 17.08098668021431 23.20311664149974 38.13728008835866 21.578616589927293 26 15.798722669005471 35.78089974380346 29.342672324046664 19.077705263144406 27 17.276897914102726 34.07042425865408 30.71462754763084 17.938050279612355 28 14.103815015718386 29.678437784088906 41.26558144570703 14.952165754485684 29 15.167488729338189 24.1964468185566 40.49923410111146 20.136830350993748 30 18.027100840470727 29.216015519142346 33.355649361174336 19.401234279212588 31 29.991524180430527 26.06285688293567 24.811664470299714 19.13395446633408 32 32.309081043057546 26.43449882792805 26.003404422161164 15.25301570685324 33 29.131769844661225 28.17764754044273 24.693976786632934 17.99660582826311 34 20.362275619814326 27.783839052963298 28.753016667830515 23.100868659391853 35 21.578552524775596 25.781738997290688 30.2460550281214 22.39365344981232 36 31.21920468239381 23.974973589141214 26.754824586411395 18.050997142053582 37 22.219139976590593 32.70916791540325 27.908573903316718 17.16311820468944 38 20.95635177149754 33.02103707386264 23.35181185858771 22.670799296052113 39 21.541010345881347 31.87254109939644 26.546356582790438 20.040091971931776 40 23.995858828594326 25.766491491186876 25.79974130491745 24.437908375301344 41 17.173945215326178 25.021157516347824 26.78269292739945 31.02220434092655 42 23.139756206471734 26.121796822496606 23.341881760074727 27.396565210956936 43 23.235085152196376 27.57556324479743 24.90564804783873 24.283703555167463 44 20.69708010258112 32.37820734173825 26.462687494674586 20.462025061006038 45 17.575633716464296 37.98172990003915 21.723275702458373 22.71936068103819 46 22.136880321812068 34.19547943476598 24.853883405267823 18.81375683815413 47 24.128409627454737 26.5797345268244 26.823118038120047 22.468737807600817 48 24.563540137778514 22.45810299241917 29.689456990180734 23.28889987962158 49 20.653515799427385 23.96888739973003 32.163525018402716 23.21407178243987 50 19.072964441918856 33.39242275824823 26.049659461686158 21.484953338146763 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 558.0 1 1162.0 2 1766.0 3 9258.5 4 16751.0 5 12920.0 6 9089.0 7 9518.0 8 9947.0 9 10730.0 10 11513.0 11 11722.0 12 11931.0 13 11341.0 14 10751.0 15 10061.5 16 9372.0 17 8764.0 18 8156.0 19 7623.5 20 7091.0 21 6748.5 22 6406.0 23 6760.5 24 7115.0 25 8533.5 26 9952.0 27 11305.5 28 12659.0 29 15500.0 30 18341.0 31 20866.5 32 23392.0 33 26062.0 34 28732.0 35 32072.5 36 35413.0 37 47085.5 38 58758.0 39 83868.0 40 108978.0 41 136249.5 42 163521.0 43 167700.5 44 171880.0 45 162928.0 46 153976.0 47 138926.5 48 123877.0 49 117994.0 50 112111.0 51 103122.5 52 94134.0 53 83519.0 54 72904.0 55 65497.0 56 58090.0 57 55386.0 58 52682.0 59 51833.5 60 50985.0 61 43608.5 62 36232.0 63 30198.0 64 24164.0 65 19735.5 66 15307.0 67 12726.0 68 10145.0 69 8182.5 70 6220.0 71 4689.0 72 3158.0 73 2660.5 74 2163.0 75 1696.0 76 1229.0 77 986.0 78 743.0 79 602.0 80 461.0 81 318.5 82 176.0 83 117.0 84 58.0 85 37.5 86 17.0 87 11.0 88 5.0 89 3.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1560911.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.38316198553151 #Duplication Level Percentage of deduplicated Percentage of total 1 77.78527480488509 13.521540320224007 2 6.176205288140549 2.1472395395928694 3 2.0167190463410654 1.0517086158556008 4 1.1422899292338955 0.7942644349725652 5 0.7905783090685641 0.6871375404393221 6 0.5887672722386553 0.6140782119062445 7 0.5222526271708311 0.6354881410835965 8 0.42842160209270325 0.5957857685822708 9 0.3785816174560242 0.5922851022884333 >10 7.033566087644755 31.162883899590405 >50 2.3524218038607025 28.902478501912388 >100 0.7802355126918433 17.884643511158703 >500 0.0031240661168842553 0.34219661107305355 >1k 7.810165292210638E-4 0.3179624928121767 >5k 7.810165292210638E-4 0.750307308508324 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5608 0.3592773707149223 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 5445 0.3488347509883651 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2442 0.1564471004432668 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2242 0.1436340701039329 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0062783848662736055 0.0 2 0.0 0.0 0.0 0.03299355312378476 0.0 3 0.0 0.0 0.0 0.04683162589026536 0.0 4 0.0 0.0 0.0 0.07117638353499975 0.0 5 0.0 0.0 0.0 0.1161501200260617 0.0 6 0.0 0.0 0.0 0.18130437930157453 0.0 7 0.0 0.0 0.0 0.2291610476189866 0.0 8 0.0 0.0 0.0 0.44480434822997594 0.0 9 0.0 0.0 0.0 0.5393645121342601 0.0 10 0.0 0.0 0.0 0.6950428307571668 0.0 11 0.0 0.0 0.0 0.9195911874539933 0.0 12 0.0 0.0 0.0 1.0937843349172374 0.0 13 0.0 0.0 0.0 1.170790647256634 0.0 14 0.0 0.0 0.0 1.1961604473285152 0.0 15 0.0 0.0 0.0 1.2443374414044106 0.0 16 0.0 0.0 0.0 1.3589499977897523 0.0 17 0.0 0.0 0.0 1.4844536299635278 0.0 18 0.0 0.0 0.0 1.6604406016742788 0.0 19 0.0 0.0 0.0 1.744109689790129 0.0 20 0.0 0.0 0.0 1.8265615400237425 0.0 21 0.0 0.0 0.0 1.9439929630837376 0.0 22 0.0 0.0 0.0 2.054761610367279 0.0 23 0.0 0.0 0.0 2.190579731964218 0.0 24 0.0 0.0 0.0 2.2888556746669093 0.0 25 0.0 0.0 0.0 2.369129309742836 0.0 26 0.0 0.0 0.0 2.454784417561283 0.0 27 0.0 0.0 0.0 2.5300609708048696 0.0 28 0.0 0.0 0.0 2.6142425801342934 0.0 29 0.0 0.0 0.0 2.6940036939966467 0.0 30 0.0 0.0 0.0 2.795739154890958 0.0 31 0.0 0.0 0.0 2.9106720370347827 0.0 32 0.0 0.0 0.0 3.0074744812484506 0.0 33 0.0 0.0 0.0 3.099856429995048 0.0 34 0.0 0.0 0.0 3.204923278777586 0.0 35 0.0 0.0 0.0 3.3474041761509783 0.0 36 0.0 0.0 0.0 3.468999834071257 0.0 37 0.0 0.0 0.0 3.5817545010573952 0.0 38 0.0 0.0 0.0 3.689448021059497 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTTAG 55 1.8189894E-12 44.000004 2 TCGTTAA 35 1.4465331E-7 44.0 1 ACCGGTT 30 2.5283025E-6 44.0 24 GTACCCG 20 7.857349E-4 44.0 22 GGTAATC 25 4.4435754E-5 44.0 8 CGTATAT 30 2.5283025E-6 44.0 42 ACGCACG 20 7.857349E-4 44.0 10 CACATCG 20 7.857349E-4 44.0 28 GCACGAT 30 2.5283025E-6 44.0 9 CACGATC 25 4.4435754E-5 44.0 43 CGCCTAT 45 4.802132E-10 44.0 17 ACGATTC 40 8.3164196E-9 44.0 9 AATGCGC 30 2.5283025E-6 44.0 34 ATATCGA 30 2.5283025E-6 44.0 1 CCCGTAC 25 4.4435754E-5 44.0 36 CCGTACG 25 4.4435754E-5 44.0 42 CGTAGTA 20 7.857349E-4 44.0 39 GTTCGAC 20 7.857349E-4 44.0 42 TACGATT 20 7.857349E-4 44.0 18 CGCTAGC 20 7.857349E-4 44.0 44 >>END_MODULE