FastQCFastQC Report
Thu 26 May 2016
SRR1546386_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546386_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences600211
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21380.3562080668298315No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15750.26240771995181694No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.2122586890276919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAG850.045.0000042
TTGGCCG207.030589E-445.036
TCGTTAA351.2106284E-745.01
GCGACAA351.2106284E-745.01
CGTATGG253.8885708E-545.02
CGACGTC207.030589E-445.018
ACGTTCA253.8885708E-545.022
CGTGATA253.8885708E-545.043
GACGTCG207.030589E-445.019
GTCTCGA207.030589E-445.026
GCACGTA207.030589E-445.011
CACGATC207.030589E-445.043
GATCACG253.8885708E-545.09
CGATTCA253.8885708E-545.010
TAACGCA253.8885708E-545.033
CGACAAG453.8380676E-1045.02
TATGCGA253.8885708E-545.01
GTAATCC207.030589E-445.040
TATGACG207.030589E-445.01
GCATTTC253.8885708E-545.026