##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546386_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	600211
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05646847525287	31.0	31.0	33.0	30.0	33.0
2	31.19674081281416	31.0	31.0	33.0	30.0	34.0
3	31.12186880946867	31.0	31.0	34.0	28.0	34.0
4	35.06134176148055	35.0	35.0	37.0	32.0	37.0
5	32.78684995776485	35.0	35.0	37.0	28.0	37.0
6	33.98402395157703	35.0	35.0	37.0	28.0	37.0
7	15.224695981913028	0.0	0.0	35.0	0.0	37.0
8	24.89737775548932	17.0	17.0	35.0	17.0	37.0
9	32.77887442915908	32.0	32.0	37.0	27.0	39.0
10	35.65334690633794	37.0	35.0	37.0	32.0	39.0
11	36.55065302035451	37.0	35.0	39.0	32.0	39.0
12	36.19240233851096	37.0	35.0	39.0	32.0	39.0
13	35.59721831156043	37.0	35.0	39.0	30.0	39.0
14	36.56596097039208	38.0	35.0	40.0	31.0	41.0
15	37.06944724438572	39.0	35.0	41.0	32.0	41.0
16	37.21680875558762	39.0	35.0	41.0	32.0	41.0
17	37.219136270411575	39.0	35.0	41.0	32.0	41.0
18	37.11615915069867	38.0	35.0	40.0	32.0	41.0
19	37.03058424454067	38.0	35.0	40.0	32.0	41.0
20	37.10321870142333	39.0	35.0	40.0	32.0	41.0
21	36.91528145935346	39.0	35.0	40.0	32.0	41.0
22	36.95398951368769	39.0	35.0	40.0	32.0	41.0
23	36.97779447560941	38.0	35.0	40.0	32.0	41.0
24	36.809387032227	38.0	35.0	40.0	32.0	41.0
25	36.56033628174092	38.0	35.0	40.0	31.0	41.0
26	36.38030792504636	38.0	35.0	40.0	31.0	41.0
27	36.27163280912879	38.0	35.0	40.0	31.0	41.0
28	36.2205407764936	38.0	35.0	40.0	31.0	41.0
29	36.30473116953871	38.0	35.0	40.0	31.0	41.0
30	36.10877174860174	38.0	35.0	40.0	30.0	41.0
31	35.91489492861677	38.0	34.0	40.0	30.0	41.0
32	35.76092574111438	38.0	34.0	40.0	30.0	41.0
33	35.539788507708124	38.0	34.0	40.0	29.0	41.0
34	35.383755046142106	38.0	34.0	40.0	28.0	41.0
35	35.18710586776984	38.0	34.0	40.0	27.0	41.0
36	34.952155158769166	38.0	34.0	40.0	25.0	41.0
37	34.75550264823537	37.0	33.0	40.0	25.0	41.0
38	34.7461342761129	37.0	33.0	40.0	25.0	41.0
39	34.67130225870569	37.0	33.0	40.0	24.0	41.0
40	34.47834844746264	37.0	33.0	40.0	23.0	41.0
41	34.391110792704566	37.0	33.0	40.0	23.0	41.0
42	34.358532249492264	37.0	33.0	40.0	23.0	41.0
43	34.24451901081453	37.0	33.0	40.0	23.0	41.0
44	34.022583724723475	36.0	33.0	40.0	23.0	41.0
45	33.92462317418374	36.0	33.0	40.0	23.0	41.0
46	33.93512781338563	36.0	33.0	40.0	23.0	41.0
47	33.90631961093682	36.0	33.0	40.0	23.0	41.0
48	33.90556654243258	36.0	33.0	40.0	23.0	41.0
49	33.82644936530654	36.0	33.0	40.0	23.0	41.0
50	33.676735348069265	36.0	33.0	40.0	23.0	41.0
51	33.47250883439324	35.0	33.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	11.0
15	28.0
16	62.0
17	208.0
18	385.0
19	751.0
20	1288.0
21	2104.0
22	3313.0
23	4821.0
24	7045.0
25	9382.0
26	11117.0
27	12276.0
28	12810.0
29	14676.0
30	18124.0
31	23425.0
32	31817.0
33	50140.0
34	62911.0
35	60856.0
36	64717.0
37	99185.0
38	92360.0
39	16395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.70973707579501	24.1666680550673	31.09089970027207	16.032695168865615
2	31.78882093130582	25.001374516628317	30.655386189190136	12.554418362875722
3	28.849521251693155	23.070886738163747	34.84891146613441	13.230680544008692
4	26.244937197085694	27.745242922905444	31.761997031044082	14.24782284896478
5	21.197878745974332	36.4923335293755	28.878177840792656	13.43160988385751
6	21.313504750829292	39.60657168895605	28.117445365046624	10.962478195168032
7	37.33670325935379	57.35199788074527	4.579722797482885	0.7315760624180496
8	87.80462204124882	3.264518644276763	7.444048842823607	1.4868104716508028
9	83.88616669804452	5.120199396545548	8.680447376006105	2.3131865294038265
10	59.54905858106566	19.72373048811168	12.487608524335608	8.239602406487052
11	55.295054572475344	17.07432886101721	17.960017393883152	9.670599172624293
12	48.445629953466366	20.22871956695229	20.734208470021375	10.591442009559971
13	20.91547805688333	45.518326055337205	21.207875230543927	12.358320657235538
14	13.179531864627606	47.403996261314774	26.87588198150317	12.540589892554452
15	12.321500272404204	22.09056481803899	53.70777943090014	11.880155478656672
16	13.86462427379705	15.634335258767335	52.528027643612	17.973012823823623
17	14.6206917234106	16.378906751125854	30.457122578559876	38.54327894690367
18	21.679875910304876	22.541572880203795	34.77410443993862	21.004446769552708
19	30.97377422273167	22.776823483741552	26.34773438007634	19.901667913450435
20	34.4838731712681	21.82849031423949	24.2281464351703	19.459490079322105
21	22.43561014376611	27.73891181601137	27.701924823103873	22.123553217118648
22	21.7906702809512	26.362395890778412	25.136327058317825	26.710606769952566
23	20.39949284501617	29.90015177995738	24.444903542254306	25.255451832772142
24	19.929658070245296	22.759662851897083	40.44194458282171	16.868734495035913
25	16.91805048557924	23.478410092450822	38.31702517947855	21.286514242491393
26	15.599514170849918	36.58613387625352	29.05778134689301	18.756570606003557
27	17.024346438169243	35.34640318154782	30.136235423875934	17.493014956406995
28	13.973919171757931	30.336831547572434	40.890453523844116	14.798795756825516
29	14.92108608472687	24.838765034296273	40.21752350423434	20.02262537674251
30	18.010832857111918	30.203545086644528	32.74198573501652	19.043636321227037
31	30.626063167785993	26.29108763418198	24.258469105031395	18.824380093000627
32	33.21282015824435	26.284589919211744	25.669972726257935	14.832617196285971
33	30.04660027890192	28.090121640556404	24.036880363738753	17.826397716802926
34	20.493793016122662	27.81205276144556	28.180256609758903	23.513897612672878
35	21.810996466242706	25.745779400910678	30.207543680472366	22.235680452374247
36	31.754499667616887	24.287625518359377	26.246936493999613	17.710938320024123
37	22.0747370508038	33.82427179775112	27.432686172029502	16.66830497941557
38	20.72437859352794	34.12016774101107	23.01923823455418	22.136215430906798
39	21.63672441857947	32.64668591545307	26.202785353817244	19.513804312150228
40	24.542535874884	25.824251804781984	25.381407538349016	24.251804781985
41	17.07299599640793	25.05868769482732	26.53900045150789	31.329315857256862
42	22.870124006391084	25.869569201497473	22.97458727014333	28.285719521968105
43	22.9041120539277	27.304397953386395	24.838098601991632	24.95339139069427
44	20.33734803260853	32.50856782031652	26.885045425691967	20.26903872138298
45	17.21694537421007	39.0452690803734	21.48777679849253	22.250008746924
46	21.866310347527786	35.06850091051314	24.376261014876434	18.688927727082643
47	23.83728388849921	26.856055620440145	26.35956355348369	22.94709693757695
48	24.250138701223403	22.74716724618509	30.153729271872727	22.84896478071878
49	20.691556802524445	24.447236055320545	32.32613197692145	22.535075165233557
50	19.068460924574858	34.53418881026839	25.512694702363003	20.88465556279375
51	18.14728487148686	36.73841365786365	22.789985521758183	22.324315948891307
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	241.0
1	445.0
2	649.0
3	2961.5
4	5274.0
5	4186.0
6	3098.0
7	3253.5
8	3409.0
9	3634.0
10	3859.0
11	3841.0
12	3823.0
13	3680.0
14	3537.0
15	3403.0
16	3269.0
17	3139.5
18	3010.0
19	2774.0
20	2538.0
21	2504.5
22	2471.0
23	2517.5
24	2564.0
25	2887.5
26	3831.0
27	4451.0
28	5647.5
29	6844.0
30	7971.5
31	9099.0
32	10078.0
33	11057.0
34	11711.5
35	12366.0
36	16227.5
37	20089.0
38	28104.0
39	36119.0
40	46630.5
41	57142.0
42	62133.5
43	67125.0
44	63611.0
45	60097.0
46	55140.5
47	50184.0
48	46996.5
49	43809.0
50	40889.5
51	37970.0
52	34043.0
53	30116.0
54	27531.0
55	24946.0
56	23172.5
57	21399.0
58	20517.0
59	19635.0
60	18031.5
61	16428.0
62	13642.5
63	10857.0
64	9114.0
65	7371.0
66	6012.5
67	4654.0
68	3824.5
69	2995.0
70	2443.0
71	1891.0
72	1476.5
73	1062.0
74	871.5
75	539.5
76	398.0
77	311.5
78	225.0
79	184.0
80	143.0
81	108.0
82	73.0
83	46.5
84	20.0
85	12.0
86	4.0
87	4.5
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	600211.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.360028512531283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.6665733131921	18.18879854842367
2	5.611593708173012	2.733971654636714
3	2.6603906140626292	1.9442157363910875
4	1.7590988426812078	1.714067918563
5	1.3008782866776722	1.5844716077400467
6	1.0252905354615462	1.4985664006483042
7	0.86702261647701	1.4784486960872607
8	0.7476168359821487	1.4569573952778854
9	0.6982921378708877	1.5309374749750124
>10	9.970630930071005	55.62316992146541
>50	0.6395957416772637	9.7268458534899
>100	0.05097734391689138	1.6938282279849126
>500	0.0	0.0
>1k	0.002039093756675655	0.8257205643167898
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2138	0.3562080668298315	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1575	0.26240771995181694	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1274	0.2122586890276919	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0061644988179157	0.0
2	0.0	0.0	0.0	0.028489981023340126	0.0
3	0.0	0.0	0.0	0.03682038483133431	0.0
4	0.0	0.0	0.0	0.05614692166588083	0.0
5	0.0	0.0	0.0	0.09696590032505235	0.0
6	0.0	0.0	0.0	0.1354523659179855	0.0
7	0.0	0.0	0.0	0.16260948233204656	0.0
8	0.0	0.0	0.0	0.34687801456487805	0.0
9	0.0	0.0	0.0	0.4186860953897879	0.0
10	0.0	0.0	0.0	0.5423092879004217	0.0
11	0.0	0.0	0.0	0.7187472405537386	0.0
12	0.0	0.0	0.0	0.8625300102797183	0.0
13	0.0	0.0	0.0	0.9251746469158346	0.0
14	0.0	0.0	0.0	0.9499992502636573	0.0
15	0.0	0.0	0.0	0.9894853643135497	0.0
16	0.0	0.0	0.0	1.0817862385061254	0.0
17	0.0	0.0	0.0	1.178418922678858	0.0
18	0.0	0.0	0.0	1.3255338539280352	0.0
19	0.0	0.0	0.0	1.3966755024483057	0.0
20	0.0	0.0	0.0	1.4649848136738581	0.0
21	0.0	0.0	0.0	1.5602846332373115	0.0
22	0.0	0.0	0.0	1.6517524670490877	0.0
23	0.0	0.0	0.0	1.7700442011226052	0.0
24	0.0	0.0	0.0	1.8510157261363087	0.0
25	0.0	0.0	0.0	1.918158780828742	0.0
26	0.0	0.0	0.0	1.9904666858821314	0.0
27	0.0	0.0	0.0	2.0531113225182476	0.0
28	0.0	0.0	0.0	2.1250860114193175	0.0
29	0.0	0.0	0.0	2.1953946195587886	0.0
30	0.0	0.0	0.0	2.288028709903684	0.0
31	0.0	0.0	0.0	2.386827299066495	0.0
32	0.0	0.0	0.0	2.469798120994117	0.0
33	0.0	0.0	0.0	2.549103565246222	0.0
34	0.0	0.0	0.0	2.643237128276556	0.0
35	0.0	0.0	0.0	2.7631949431116722	0.0
36	0.0	0.0	0.0	2.878154515661992	0.0
37	0.0	0.0	0.0	2.9796188340433614	0.0
38	0.0	0.0	0.0	3.083415665490969	0.0
39	0.0	0.0	0.0	3.1747168912265855	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAAG	85	0.0	45.000004	2
TTGGCCG	20	7.030589E-4	45.0	36
TCGTTAA	35	1.2106284E-7	45.0	1
GCGACAA	35	1.2106284E-7	45.0	1
CGTATGG	25	3.8885708E-5	45.0	2
CGACGTC	20	7.030589E-4	45.0	18
ACGTTCA	25	3.8885708E-5	45.0	22
CGTGATA	25	3.8885708E-5	45.0	43
GACGTCG	20	7.030589E-4	45.0	19
GTCTCGA	20	7.030589E-4	45.0	26
GCACGTA	20	7.030589E-4	45.0	11
CACGATC	20	7.030589E-4	45.0	43
GATCACG	25	3.8885708E-5	45.0	9
CGATTCA	25	3.8885708E-5	45.0	10
TAACGCA	25	3.8885708E-5	45.0	33
CGACAAG	45	3.8380676E-10	45.0	2
TATGCGA	25	3.8885708E-5	45.0	1
GTAATCC	20	7.030589E-4	45.0	40
TATGACG	20	7.030589E-4	45.0	1
GCATTTC	25	3.8885708E-5	45.0	26
>>END_MODULE