FastQCFastQC Report
Thu 26 May 2016
SRR1546385_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546385_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences118266
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA3770.31877293558588266No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.2528199144301828No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.10653949571305361No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.10315728949994081No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATA207.8278274E-444.0000041
TTAGGGT207.8278274E-444.0000045
CTCACCA207.8278274E-444.00000431
CACGGGG207.8278274E-444.0000044
AGTGGTA207.8278274E-444.00000429
TATGCCA207.8278274E-444.00000410
ACGGGCC207.8278274E-444.0000045
ACGGGAC207.8278274E-444.0000045
ATCAATA207.8278274E-444.00000415
GTAATGG207.8278274E-444.0000042
TGCATAG408.203642E-944.0000042
AGGCCGA207.8278274E-444.0000047
TGTCTAT207.8278274E-444.00000417
CATAGCC207.8278274E-444.00000436
AATCTGC207.8278274E-444.00000430
CAGTGGT207.8278274E-444.00000428
CATAAGC207.8278274E-444.00000432
TAGATAG207.8278274E-444.0000042
TTACGGG207.8278274E-444.0000043
GCAATGG207.8278274E-444.0000042
Produced by FastQC (version 0.11.5)