##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546384_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	44101
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.004285617106188	31.0	31.0	33.0	30.0	33.0
2	31.17090315412349	31.0	31.0	33.0	30.0	34.0
3	31.122627604816216	31.0	31.0	34.0	28.0	34.0
4	35.09156254960205	35.0	35.0	37.0	32.0	37.0
5	32.70245572662751	35.0	35.0	37.0	28.0	37.0
6	33.934876760164165	35.0	35.0	37.0	28.0	37.0
7	14.943357293485409	0.0	0.0	35.0	0.0	37.0
8	24.721389537652207	17.0	17.0	35.0	17.0	37.0
9	32.6589419741049	32.0	32.0	37.0	27.0	39.0
10	35.506133647763086	37.0	35.0	37.0	32.0	39.0
11	36.435092174780614	37.0	35.0	39.0	32.0	39.0
12	36.17307997551076	37.0	35.0	39.0	32.0	39.0
13	35.66236593274529	37.0	35.0	39.0	31.0	39.0
14	36.66422530101358	39.0	35.0	40.0	31.0	41.0
15	37.14255912564341	39.0	35.0	40.0	32.0	41.0
16	37.25845218929276	39.0	35.0	41.0	32.0	41.0
17	37.25126414367021	39.0	35.0	41.0	32.0	41.0
18	37.15720731956191	39.0	35.0	40.0	32.0	41.0
19	37.08426112786558	39.0	35.0	40.0	32.0	41.0
20	37.218249019296614	39.0	35.0	40.0	32.0	41.0
21	37.04621210403392	39.0	35.0	40.0	32.0	41.0
22	37.11591573887213	39.0	35.0	40.0	32.0	41.0
23	37.084737307544046	39.0	35.0	40.0	32.0	41.0
24	36.91664588104578	38.0	35.0	40.0	32.0	41.0
25	36.673884945919596	38.0	35.0	40.0	31.0	41.0
26	36.52706287839278	38.0	35.0	40.0	31.0	41.0
27	36.411850071426954	38.0	35.0	40.0	31.0	41.0
28	36.38123852066847	38.0	35.0	40.0	31.0	41.0
29	36.42554590598853	38.0	35.0	40.0	31.0	41.0
30	36.15235482188613	38.0	35.0	40.0	30.0	41.0
31	36.04387655608716	38.0	35.0	40.0	30.0	41.0
32	35.911861409038345	38.0	34.0	40.0	30.0	41.0
33	35.74227341783633	38.0	34.0	40.0	29.0	41.0
34	35.654157502097455	38.0	34.0	40.0	29.0	41.0
35	35.449944445704176	38.0	34.0	40.0	27.0	41.0
36	35.2441667989388	38.0	34.0	40.0	27.0	41.0
37	35.047776694406025	38.0	34.0	40.0	26.0	41.0
38	35.02848007981678	38.0	34.0	40.0	26.0	41.0
39	34.9336976485794	38.0	34.0	40.0	26.0	41.0
40	34.78483481100202	37.0	34.0	40.0	24.0	41.0
41	34.667921362327384	37.0	34.0	40.0	24.0	41.0
42	34.668080088886875	37.0	33.0	40.0	24.0	41.0
43	34.56731139883449	37.0	33.0	40.0	24.0	41.0
44	34.318722931452804	37.0	33.0	40.0	23.0	41.0
45	34.20103852520351	36.0	33.0	40.0	23.0	41.0
46	34.22178635405093	36.0	33.0	40.0	23.0	41.0
47	34.21176390558037	36.0	33.0	40.0	23.0	41.0
48	34.1630575270402	36.0	33.0	40.0	23.0	41.0
49	34.117525679689805	36.0	33.0	40.0	24.0	41.0
50	33.98380989093218	36.0	33.0	40.0	24.0	41.0
51	33.74977891657785	36.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	4.0
16	8.0
17	10.0
18	24.0
19	60.0
20	101.0
21	143.0
22	210.0
23	350.0
24	488.0
25	617.0
26	733.0
27	776.0
28	898.0
29	1075.0
30	1303.0
31	1736.0
32	2327.0
33	3594.0
34	4372.0
35	4512.0
36	5056.0
37	7637.0
38	6854.0
39	1212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.525362236683975	23.95864039364187	29.16260402258452	15.353393347089636
2	29.595700777760143	23.98131561642593	33.28042448017052	13.14255912564341
3	29.37121607219791	24.060678896170153	33.28042448017052	13.287680551461417
4	26.362214008752638	27.78395047731344	30.126300990907236	15.72753452302669
5	21.833972018775086	35.91301784540033	29.094578354232333	13.158431781592252
6	21.55279925625269	39.97188272374776	27.31457336568332	11.160744654316229
7	37.31888165801229	57.93292669100474	3.866125484682887	0.8820661663000838
8	89.23153669984808	3.0339448085077434	6.401215391941226	1.3333030997029547
9	86.34498083943674	3.7686220267114123	7.362644837985534	2.5237522958663066
10	58.12793360694769	20.316999614521215	11.512210607469218	10.042856171061882
11	50.708600712001996	17.849935375615065	19.543774517584637	11.897689394798304
12	44.79263508763974	20.25124146844743	21.98816353370672	12.967959910206117
13	21.153715335253167	42.368653772023315	22.770458719756924	13.707172172966601
14	14.255912564340944	43.570440579578694	27.82023083376794	14.353416022312418
15	12.827373528944921	21.870252375229587	50.66325026643387	14.639123829391623
16	15.04274279494796	15.605088319992744	49.32994716673092	20.02222171832838
17	15.793292669100472	15.995102151878642	29.66599396839074	38.54561121063014
18	21.36686242942337	20.761433981088864	34.811002018094825	23.06070157139294
19	30.595678102537356	22.39405002154146	25.12187932246434	21.888392553456836
20	33.189723589034266	20.843064783111494	24.394004671095892	21.57320695675835
21	23.126459717466723	26.194417360150563	27.87238384617129	22.806739076211425
22	23.6502573637786	23.71148046529557	24.70238770095916	27.935874469966667
23	19.675290809732203	30.398403664316003	24.731865490578446	25.19444003537335
24	20.430375728441533	22.040316546110063	39.289358517947434	18.239949207500963
25	17.623183147774427	23.41670256910274	37.79732885875603	21.162785424366795
26	17.38509330854176	32.85866533638693	28.366703702863884	21.389537652207434
27	17.64132332600168	33.137570576630914	28.879163737783724	20.341942359583683
28	15.899866216185574	26.88601165506451	39.931067322736446	17.28305480601347
29	15.623228498219996	22.430330377995965	39.436747466043855	22.509693657740186
30	18.924740935579692	29.17167411169815	32.105847939956014	19.797737012766152
31	29.89047867395297	25.598059000929684	24.831636470828325	19.679825854289017
32	32.54347973968844	24.811228770322668	24.849776649055578	17.795514840933315
33	29.362145983084282	26.04476088977574	24.511915829573024	20.08117729756695
34	20.611777510714045	26.656991904945464	27.94040951452348	24.790821069817014
35	20.967778508423844	24.777215936146575	31.339425409854655	22.91558014557493
36	32.25550441033083	23.530078683023063	25.06065622094737	19.153760685698735
37	21.775016439536518	32.1013128953992	27.568535860864834	18.55513480419945
38	21.110632411963447	32.616040452597446	23.822589056937485	22.450738078501622
39	21.183193124872453	31.883630756672183	26.48012516722977	20.453050951225595
40	25.5323008548559	24.92233736196458	24.876986916396454	24.668374866783065
41	18.335185143194032	23.45071540327884	26.065168590281402	32.14893086324573
42	22.854357044057956	24.473367950840117	23.246638398222263	29.425636606879664
43	23.66386249744904	25.50055554295821	25.276070837395974	25.559511122196778
44	20.49613387451532	29.144463844357272	28.276002811727626	22.083399469399787
45	16.893040974127572	37.99460329697739	22.38724745470624	22.725108274188795
46	21.677512981565044	33.15117571030136	25.319153760685698	19.852157547447906
47	22.0879345139566	26.815718464433914	26.89281422189973	24.203532799709755
48	23.915557470352148	22.380444887871022	31.518559669848756	22.185437971928074
49	21.067549488673727	22.725108274188795	33.194258633591076	23.013083603546406
50	19.797737012766152	33.380195460420396	26.130926736355185	20.691140790458267
51	19.088002539624952	34.99693884492415	23.26704609872792	22.648012516722975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	28.5
2	51.0
3	185.0
4	319.0
5	246.5
6	174.0
7	182.5
8	191.0
9	188.0
10	185.0
11	182.5
12	180.0
13	188.0
14	196.0
15	180.0
16	164.0
17	161.0
18	158.0
19	151.5
20	145.0
21	147.0
22	149.0
23	163.0
24	177.0
25	206.0
26	284.5
27	334.0
28	395.0
29	456.0
30	558.0
31	660.0
32	767.0
33	874.0
34	932.5
35	991.0
36	1248.0
37	1505.0
38	1990.0
39	2475.0
40	3144.5
41	3814.0
42	4163.0
43	4512.0
44	4321.0
45	4130.0
46	3789.5
47	3449.0
48	3338.0
49	3227.0
50	3066.5
51	2906.0
52	2687.0
53	2468.0
54	2305.5
55	2143.0
56	2015.5
57	1888.0
58	1717.0
59	1546.0
60	1422.0
61	1298.0
62	1160.5
63	1023.0
64	861.0
65	699.0
66	606.5
67	514.0
68	425.0
69	336.0
70	279.0
71	222.0
72	173.0
73	124.0
74	106.5
75	64.5
76	40.0
77	35.0
78	30.0
79	22.0
80	14.0
81	8.5
82	3.0
83	2.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	44101.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.30935806444298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.77803276094009	37.12387474206934
2	16.756350399620164	19.205913698102083
3	8.835166574345177	15.190131743044374
4	4.823138403102003	11.056438629509536
5	2.239455566985835	6.417088047890071
6	1.2977763709741237	4.462483843903766
7	0.6488881854870618	2.60311557561053
8	0.28487773996992954	1.306092832362078
9	0.1582654110944053	0.8163080202262987
>10	0.16617868164912558	1.1224235278111607
>50	0.007913270554720265	0.3696061313802408
>100	0.003956635277360132	0.3265232080905195
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	144	0.3265232080905195	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82	0.1859368268293236	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81	0.1836693045509172	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004535044556812771	0.0
2	0.0	0.0	0.0	0.013605133670438313	0.0
3	0.0	0.0	0.0	0.02494274506247024	0.0
4	0.0	0.0	0.0	0.05215301240334686	0.0
5	0.0	0.0	0.0	0.08843336885784903	0.0
6	0.0	0.0	0.0	0.12244620303394481	0.0
7	0.0	0.0	0.0	0.1519239926532278	0.0
8	0.0	0.0	0.0	0.2970454184712365	0.0
9	0.0	0.0	0.0	0.346930908596177	0.0
10	0.0	0.0	0.0	0.41949162150518127	0.0
11	0.0	0.0	0.0	0.494319856692592	0.0
12	0.0	0.0	0.0	0.5782181809936283	0.0
13	0.0	0.0	0.0	0.6054284483345049	0.0
14	0.0	0.0	0.0	0.6190335820049432	0.0
15	0.0	0.0	0.0	0.6439763270674135	0.0
16	0.0	0.0	0.0	0.7029319063059795	0.0
17	0.0	0.0	0.0	0.7800276637717966	0.0
18	0.0	0.0	0.0	0.870728554908052	0.0
19	0.0	0.0	0.0	0.9024738668057414	0.0
20	0.0	0.0	0.0	0.9319516564250244	0.0
21	0.0	0.0	0.0	0.977302101993152	0.0
22	0.0	0.0	0.0	1.024920069839686	0.0
23	0.0	0.0	0.0	1.077073082243033	0.0
24	0.0	0.0	0.0	1.111085916419129	0.0
25	0.0	0.0	0.0	1.1496337951520375	0.0
26	0.0	0.0	0.0	1.1995192852769778	0.0
27	0.0	0.0	0.0	1.2720799981859823	0.0
28	0.0	0.0	0.0	1.3196979660325163	0.0
29	0.0	0.0	0.0	1.3491757556517994	0.0
30	0.0	0.0	0.0	1.4103988571687718	0.0
31	0.0	0.0	0.0	1.4897621369129952	0.0
32	0.0	0.0	0.0	1.5577878052651868	0.0
33	0.0	0.0	0.0	1.6167433845037527	0.0
34	0.0	0.0	0.0	1.6734314414639124	0.0
35	0.0	0.0	0.0	1.7459921543729167	0.0
36	0.0	0.0	0.0	1.811750300446702	0.0
37	0.0	0.0	0.0	1.8797759687988935	0.0
38	0.0	0.0	0.0	1.9455341148726786	0.0
39	0.0	0.0	0.0	2.006757216389651	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAGG	20	6.9596677E-4	45.000004	3
TCGCGCA	20	6.9596677E-4	45.000004	34
GGCGATA	20	6.9596677E-4	45.000004	8
GTACATG	20	6.9596677E-4	45.000004	41
ACTCCTC	20	6.9596677E-4	45.000004	33
ATGAAGG	20	6.9596677E-4	45.000004	3
ATGAAAG	20	6.9596677E-4	45.000004	2
TAATGGG	35	1.1767952E-7	45.000004	3
AGTACAT	20	6.9596677E-4	45.000004	40
CGCAGTA	20	6.9596677E-4	45.000004	37
GGTAGTT	20	6.9596677E-4	45.000004	8
GCGCAGT	20	6.9596677E-4	45.000004	36
AAGGATG	40	6.5629138E-9	45.000004	6
CGTAAGG	20	6.9596677E-4	45.000004	3
AACAGGG	20	6.9596677E-4	45.000004	4
CAGGTGG	20	6.9596677E-4	45.000004	34
TGAAAGG	35	1.1767952E-7	45.000004	3
CCTCCGC	45	3.6743586E-10	45.0	45
CGTTTTT	90	0.0	45.0	1
CAATGAT	25	3.8299193E-5	44.999996	18
>>END_MODULE