FastQCFastQC Report
Thu 26 May 2016
SRR1546383_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546383_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences125223
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.273112766823986No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA1950.15572219161016748No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.1309663560208588No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.11180054782268434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT1300.044.0000041
GTGTTTA207.8296004E-444.01
TTTAGCT207.8296004E-444.040
TTAGGAT351.4323086E-744.05
GAAGCAA207.8296004E-444.01
GCGACCC207.8296004E-444.031
TCCGCCG207.8296004E-444.016
GCGCGAC207.8296004E-444.09
TTATGGG950.044.03
TTATGAG207.8296004E-444.02
CGCGACC207.8296004E-444.010
GGATAAC254.420078E-544.08
GTAATTA207.8296004E-444.01
CTGAATT207.8296004E-444.039
TGGCATG207.8296004E-444.01
ACAGGGC302.5096178E-644.05
CCTCAGA207.8296004E-444.015
TTGACGC254.420078E-544.042
ACGTGGC207.8296004E-444.026
TATAGGC254.420078E-544.04
Produced by FastQC (version 0.11.5)