##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546383_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	125223
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.518427126007204	31.0	31.0	33.0	30.0	34.0
2	31.712065674836094	31.0	31.0	34.0	30.0	34.0
3	31.785494677495347	31.0	31.0	34.0	30.0	34.0
4	35.67474026337015	37.0	35.0	37.0	35.0	37.0
5	27.099071256877732	35.0	25.0	37.0	0.0	37.0
6	31.32218522156473	35.0	26.0	37.0	17.0	37.0
7	14.915311085024317	0.0	0.0	32.0	0.0	37.0
8	25.05134040871086	17.0	17.0	35.0	17.0	37.0
9	33.287495108726034	32.0	32.0	37.0	28.0	39.0
10	36.157910288046125	37.0	35.0	39.0	33.0	39.0
11	37.038507302971496	39.0	37.0	39.0	34.0	39.0
12	36.82609424786182	39.0	35.0	39.0	33.0	39.0
13	36.216956948803336	38.0	35.0	39.0	32.0	39.0
14	37.49226579781669	40.0	35.0	41.0	33.0	41.0
15	37.84054047579119	40.0	35.0	41.0	33.0	41.0
16	37.957819250457185	40.0	35.0	41.0	33.0	41.0
17	38.00650040328055	40.0	35.0	41.0	34.0	41.0
18	37.98454756714022	40.0	36.0	41.0	34.0	41.0
19	37.90682222914321	40.0	36.0	41.0	34.0	41.0
20	37.92456657323335	40.0	36.0	41.0	34.0	41.0
21	37.767798247925704	40.0	35.0	41.0	33.0	41.0
22	37.69329915430871	39.0	35.0	41.0	33.0	41.0
23	37.62941312698147	39.0	35.0	41.0	33.0	41.0
24	37.51001014190684	39.0	35.0	41.0	33.0	41.0
25	37.31389600951902	39.0	35.0	41.0	33.0	41.0
26	37.03417103886666	39.0	35.0	40.0	33.0	41.0
27	36.81044217116664	38.0	35.0	40.0	32.0	41.0
28	36.85388466974917	38.0	35.0	40.0	32.0	41.0
29	36.85901152344218	38.0	35.0	40.0	32.0	41.0
30	36.690935371297606	38.0	35.0	40.0	32.0	41.0
31	36.50358161040703	38.0	35.0	40.0	31.0	41.0
32	36.262898988205045	38.0	35.0	40.0	31.0	41.0
33	36.0572338947318	38.0	35.0	40.0	31.0	41.0
34	35.88513292286561	38.0	35.0	40.0	30.0	41.0
35	35.75517277177515	38.0	35.0	40.0	30.0	41.0
36	35.54554674460762	38.0	35.0	40.0	29.0	41.0
37	35.37541825383516	38.0	34.0	40.0	29.0	41.0
38	35.22416009838448	38.0	34.0	40.0	29.0	41.0
39	35.19922059046661	37.0	34.0	40.0	28.0	41.0
40	34.9770569304361	37.0	34.0	40.0	27.0	41.0
41	34.834247702099454	37.0	34.0	40.0	27.0	40.0
42	34.822524616084905	37.0	34.0	40.0	27.0	41.0
43	34.70170815265566	37.0	34.0	40.0	27.0	41.0
44	34.475863060300426	36.0	33.0	40.0	26.0	40.0
45	34.2167892479816	36.0	33.0	39.0	26.0	40.0
46	34.20885939483961	36.0	33.0	39.0	26.0	40.0
47	34.05324900377726	36.0	33.0	39.0	25.0	40.0
48	34.05796858404606	36.0	33.0	39.0	26.0	40.0
49	33.928679236242544	35.0	33.0	39.0	25.0	40.0
50	33.94273416225454	35.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	6.0
16	10.0
17	15.0
18	51.0
19	93.0
20	138.0
21	275.0
22	442.0
23	706.0
24	1007.0
25	1419.0
26	1838.0
27	2037.0
28	2177.0
29	2580.0
30	3161.0
31	4339.0
32	6113.0
33	11748.0
34	11450.0
35	13552.0
36	16309.0
37	29610.0
38	13331.0
39	2816.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.711610486891384	23.590714165927984	29.948172460330767	15.749502886849859
2	31.363247965629316	23.962850275109204	31.241864513707547	13.432037245553932
3	29.557669118292967	22.720267043594227	34.41220861982224	13.30985521829057
4	26.659639203660667	26.803382765146978	31.671498047483286	14.865479983709061
5	17.81861159691111	47.87858460506457	23.08361882401795	11.219184974006373
6	21.95443329100884	39.790613545434944	27.09486276482755	11.160090398728668
7	39.50632072382869	55.81482634979197	3.5281058591472814	1.150747067232058
8	89.73910543590236	2.501137969861767	6.2768021849021345	1.4829544093337486
9	86.8378812199037	3.0761122158070004	7.6894819641759105	2.3965246001133975
10	60.52162941312697	17.056770721033676	11.767007658337526	10.654592207501818
11	51.97288038139958	17.521541569839407	17.86013751467382	12.645440534087188
12	44.97895753974909	19.887720306972362	22.352922386462552	12.780399766816
13	20.69587855266205	41.749518858356694	23.359127316866708	14.195475272114546
14	14.37116184726448	42.10408631002292	28.79183536570758	14.732916477005023
15	13.408079985306214	20.118508580692048	52.19408575102018	14.279325682981561
16	15.32545938046525	14.224223984411811	50.053903835557364	20.396412799565574
17	16.71817477619926	15.428475599530438	28.81818835198007	39.03516127229024
18	22.591696413598143	20.45790310086805	34.34113541442068	22.609265071113136
19	30.346661555784483	21.222139702770257	25.64065706779106	22.790541673654204
20	34.59747809907125	19.76713543039218	24.382102329444272	21.25328414109229
21	23.462942111273488	26.220422765785838	27.54525925748465	22.771375865456026
22	24.191242822804117	22.262683372862814	25.686974437603315	27.859099366729755
23	20.90350814147561	28.702394927449433	24.279884685720674	26.11421224535429
24	21.748400852878465	20.177603155969752	38.83551743689259	19.238478554259203
25	17.93600217212493	22.281849181060988	37.02674428818987	22.755404358624215
26	17.77868282983158	32.66891864912995	28.014821558339918	21.537576962698544
27	18.817629349240956	31.622784951646267	29.274973447369888	20.28461225174289
28	16.01303274957476	26.057513396101356	40.046157654743936	17.88329619957995
29	17.013647652587782	22.475902989067503	39.481564888239376	21.028884470105332
30	19.305558882952813	27.765666051763656	32.30636544404782	20.622409621235715
31	31.405572458733623	24.14971690504141	24.648027918193943	19.796682718031033
32	33.0474433610439	24.35175646646383	24.611293452480773	17.9895067200115
33	30.127851912188657	25.358759972209576	24.24874024739864	20.264647868203127
34	21.475288086054476	26.530269998322993	27.199476134575917	24.794965781046614
35	22.63162518067767	24.390088082860178	30.16139207653546	22.81689465992669
36	32.33191985497872	22.19081159211966	25.676592958162637	19.800675594738987
37	22.494270221924086	30.123859035480706	27.9740942159188	19.40777652667641
38	22.52062320819658	31.588446211957866	22.28664063311053	23.604289946735022
39	21.973599099207014	30.9655574455172	25.293276794199148	21.76756666107664
40	25.22699504084713	24.636847863411674	24.307036247334754	25.829120848406443
41	19.375833513012786	23.336767207302174	25.924949889397315	31.36244939028773
42	23.664183097354318	24.055485014733716	23.936497288836716	28.34383459907525
43	25.05370419172197	24.925133561725882	23.46773356332303	26.553428683229118
44	22.000750660821094	29.5544748169266	26.382533560128728	22.06224096212357
45	18.429521733227922	36.21219743976746	21.833049839087067	23.525230987917556
46	23.576339809779352	30.871325555209506	25.50809356108702	20.04424107392412
47	23.443776303075314	26.01199460163069	26.442426710748023	24.10180238454597
48	25.546425177483368	21.603060140708973	30.09351317250026	22.757001509307393
49	21.990369181380416	21.516015428475598	32.93005278583008	23.563562604313905
50	19.981952197280055	32.83582089552239	25.767630547103966	21.414596360093594
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32.0
1	86.5
2	141.0
3	635.0
4	1129.0
5	849.5
6	570.0
7	595.5
8	621.0
9	660.5
10	700.0
11	694.5
12	689.0
13	641.5
14	594.0
15	559.0
16	524.0
17	483.5
18	443.0
19	405.5
20	368.0
21	366.5
22	365.0
23	408.0
24	451.0
25	497.5
26	544.0
27	653.5
28	763.0
29	868.0
30	973.0
31	1131.5
32	1290.0
33	1590.5
34	1891.0
35	2087.5
36	2284.0
37	2871.5
38	3459.0
39	5632.0
40	7805.0
41	9922.0
42	12039.0
43	12243.0
44	12447.0
45	11773.0
46	11099.0
47	10524.5
48	9950.0
49	9639.0
50	9328.0
51	8823.5
52	8319.0
53	7663.5
54	7008.0
55	6500.0
56	5992.0
57	5829.0
58	5666.0
59	5303.0
60	4940.0
61	4536.5
62	4133.0
63	3587.5
64	3042.0
65	2595.5
66	2149.0
67	1748.5
68	1348.0
69	1106.5
70	865.0
71	726.0
72	587.0
73	467.5
74	348.0
75	258.0
76	168.0
77	124.0
78	80.0
79	57.0
80	34.0
81	28.0
82	22.0
83	15.5
84	9.0
85	8.0
86	7.0
87	6.5
88	6.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	125223.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.276331025450595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.093589248317514	23.20979372798927
2	11.662566333895859	9.16125631872739
3	7.998698737368604	9.424786181452289
4	5.503934286237114	8.646973798743042
5	4.023748043022995	7.90190300503901
6	2.883110017689039	6.794279006252844
7	2.21824614195962	6.098719883727431
8	1.7140068722932722	5.3855921036870225
9	1.248398836996523	4.412927337629668
>10	3.6455685907732347	18.29216677447434
>50	0.0	0.0
>100	0.008132891446231421	0.6716018622776966
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	342	0.273112766823986	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	195	0.15572219161016748	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	164	0.1309663560208588	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	140	0.11180054782268434	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007985753415906023	0.0
2	0.0	0.0	0.0	0.021561534222946262	0.0
3	0.0	0.0	0.0	0.031144438322033494	0.0
4	0.0	0.0	0.0	0.039928767079530116	0.0
5	0.0	0.0	0.0	0.0686774793767918	0.0
6	0.0	0.0	0.0	0.11579342453063735	0.0
7	0.0	0.0	0.0	0.14534071216948963	0.0
8	0.0	0.0	0.0	0.3154372599282879	0.0
9	0.0	0.0	0.0	0.3761289858891737	0.0
10	0.0	0.0	0.0	0.5070953419100325	0.0
11	0.0	0.0	0.0	0.628478793831804	0.0
12	0.0	0.0	0.0	0.7362864649465354	0.0
13	0.0	0.0	0.0	0.7865967114667434	0.0
14	0.0	0.0	0.0	0.7977767662490117	0.0
15	0.0	0.0	0.0	0.8241297525215017	0.0
16	0.0	0.0	0.0	0.8912100812151122	0.0
17	0.0	0.0	0.0	0.9670747386662195	0.0
18	0.0	0.0	0.0	1.0828681631968569	0.0
19	0.0	0.0	0.0	1.146754190524105	0.0
20	0.0	0.0	0.0	1.1890786836284069	0.0
21	0.0	0.0	0.0	1.2585547383467894	0.0
22	0.0	0.0	0.0	1.3328222451147154	0.0
23	0.0	0.0	0.0	1.409485477907413	0.0
24	0.0	0.0	0.0	1.4765658066010239	0.0
25	0.0	0.0	0.0	1.5284732038044129	0.0
26	0.0	0.0	0.0	1.5779848749830303	0.0
27	0.0	0.0	0.0	1.6258993954784664	0.0
28	0.0	0.0	0.0	1.6865911214393523	0.0
29	0.0	0.0	0.0	1.7281170392020635	0.0
30	0.0	0.0	0.0	1.795197367895674	0.0
31	0.0	0.0	0.0	1.8582848198813318	0.0
32	0.0	0.0	0.0	1.9133865184510832	0.0
33	0.0	0.0	0.0	1.9748768197535596	0.0
34	0.0	0.0	0.0	2.057928655278982	0.0
35	0.0	0.0	0.0	2.148966244220311	0.0
36	0.0	0.0	0.0	2.21365084688915	0.0
37	0.0	0.0	0.0	2.283925476949123	0.0
38	0.0	0.0	0.0	2.3398257508604647	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	130	0.0	44.000004	1
GTGTTTA	20	7.8296004E-4	44.0	1
TTTAGCT	20	7.8296004E-4	44.0	40
TTAGGAT	35	1.4323086E-7	44.0	5
GAAGCAA	20	7.8296004E-4	44.0	1
GCGACCC	20	7.8296004E-4	44.0	31
TCCGCCG	20	7.8296004E-4	44.0	16
GCGCGAC	20	7.8296004E-4	44.0	9
TTATGGG	95	0.0	44.0	3
TTATGAG	20	7.8296004E-4	44.0	2
CGCGACC	20	7.8296004E-4	44.0	10
GGATAAC	25	4.420078E-5	44.0	8
GTAATTA	20	7.8296004E-4	44.0	1
CTGAATT	20	7.8296004E-4	44.0	39
TGGCATG	20	7.8296004E-4	44.0	1
ACAGGGC	30	2.5096178E-6	44.0	5
CCTCAGA	20	7.8296004E-4	44.0	15
TTGACGC	25	4.420078E-5	44.0	42
ACGTGGC	20	7.8296004E-4	44.0	26
TATAGGC	25	4.420078E-5	44.0	4
>>END_MODULE