FastQCFastQC Report
Thu 26 May 2016
SRR1546382_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546382_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences47596
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.21430372300193296No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.18068745272711995No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC650.13656609799142785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAT253.8344868E-545.00000422
CAACTAG253.8344868E-545.00000416
TACGGGG253.8344868E-545.0000044
CACAACT253.8344868E-545.00000414
GGCCGAT302.1217638E-645.0000048
AGGGCAA253.8344868E-545.0000047
CATCAAC253.8344868E-545.00000426
TACATCA253.8344868E-545.00000424
CCACAAC253.8344868E-545.00000413
TGCTAGG253.8344868E-545.0000043
AAGGATC253.8344868E-545.0000046
CTAGATA253.8344868E-545.00000419
TGGGATG253.8344868E-545.0000046
ATACATC253.8344868E-545.00000423
CCTAGGG253.8344868E-545.0000044
ACAACTA253.8344868E-545.00000415
TAGATAC253.8344868E-545.00000420
ATTTATC253.8344868E-545.00000436
GTACGGG253.8344868E-545.0000043
AACATGA253.8344868E-545.00000430