##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546382_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	47596
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98472560719388	31.0	31.0	33.0	30.0	33.0
2	31.14530632826288	31.0	31.0	33.0	30.0	34.0
3	31.133519623497772	31.0	31.0	34.0	28.0	34.0
4	35.09836961089167	35.0	35.0	37.0	32.0	37.0
5	32.80305067652744	35.0	35.0	37.0	28.0	37.0
6	33.984998739389866	35.0	35.0	37.0	28.0	37.0
7	15.289478107403983	0.0	0.0	35.0	0.0	37.0
8	24.88406588788974	17.0	17.0	35.0	17.0	37.0
9	32.737246827464496	32.0	32.0	37.0	27.0	39.0
10	35.558576350953864	37.0	35.0	37.0	32.0	39.0
11	36.42995209681486	37.0	35.0	39.0	32.0	39.0
12	36.104588620892514	37.0	35.0	39.0	32.0	39.0
13	35.62589293217918	37.0	35.0	39.0	31.0	39.0
14	36.649466341709385	39.0	35.0	40.0	31.0	41.0
15	37.10666862761577	39.0	35.0	40.0	32.0	41.0
16	37.18482645600471	39.0	35.0	41.0	32.0	41.0
17	37.226636692159005	39.0	35.0	41.0	32.0	41.0
18	37.071497604840744	39.0	35.0	40.0	32.0	41.0
19	36.976258509118416	39.0	35.0	40.0	31.0	41.0
20	37.10271871585847	39.0	35.0	40.0	32.0	41.0
21	36.929510883267504	39.0	35.0	40.0	32.0	41.0
22	36.98279267165308	39.0	35.0	40.0	32.0	41.0
23	37.01888814186066	39.0	35.0	40.0	32.0	41.0
24	36.897260273972606	38.0	35.0	40.0	32.0	41.0
25	36.62463232204387	38.0	35.0	40.0	31.0	41.0
26	36.4327884696193	38.0	35.0	40.0	31.0	41.0
27	36.27199764686108	38.0	35.0	40.0	31.0	41.0
28	36.24294058324229	38.0	35.0	40.0	30.0	41.0
29	36.28664173459955	38.0	35.0	40.0	31.0	41.0
30	36.08084713001092	38.0	35.0	40.0	30.0	41.0
31	35.95279015043281	38.0	34.0	40.0	30.0	41.0
32	35.81252626271115	38.0	34.0	40.0	30.0	41.0
33	35.618350281536266	38.0	34.0	40.0	29.0	41.0
34	35.465144129758805	38.0	34.0	40.0	28.0	41.0
35	35.28130515169342	38.0	34.0	40.0	27.0	41.0
36	35.12307756954366	38.0	34.0	40.0	26.0	41.0
37	34.90259685687873	37.0	34.0	40.0	25.0	41.0
38	34.90236574502059	37.0	34.0	40.0	26.0	41.0
39	34.780989999159594	37.0	33.0	40.0	25.0	41.0
40	34.57412387595596	37.0	33.0	40.0	24.0	41.0
41	34.50621901000084	37.0	33.0	40.0	24.0	41.0
42	34.44257920833684	37.0	33.0	40.0	24.0	41.0
43	34.32958651987562	37.0	33.0	40.0	23.0	41.0
44	34.1403059080595	36.0	33.0	40.0	23.0	41.0
45	34.01334145726532	36.0	33.0	40.0	23.0	41.0
46	34.04542398520884	36.0	33.0	40.0	23.0	41.0
47	34.01140852172451	36.0	33.0	40.0	23.0	41.0
48	34.00455920665602	36.0	33.0	40.0	23.0	41.0
49	33.88730145390369	36.0	33.0	40.0	23.0	41.0
50	33.72720396671989	35.0	33.0	40.0	23.0	41.0
51	33.54031851416085	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	5.0
16	5.0
17	12.0
18	30.0
19	59.0
20	106.0
21	158.0
22	264.0
23	377.0
24	489.0
25	680.0
26	798.0
27	906.0
28	995.0
29	1266.0
30	1452.0
31	1920.0
32	2560.0
33	3955.0
34	4891.0
35	4960.0
36	5333.0
37	7984.0
38	7049.0
39	1339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.273552399361293	24.470543743171692	29.750399193209514	15.505504664257499
2	30.571896798050258	23.800319354567613	32.0447096394655	13.583074207916631
3	30.088662912849816	22.291789225985376	34.21295907219094	13.406588788973863
4	26.82998571308513	27.68930162198504	30.34918900747962	15.131523657450204
5	21.60895873602824	36.26355155895453	28.05277754433146	14.07471216068577
6	21.688797377930918	40.03067484662577	27.180855534078496	11.09967224136482
7	38.423396924111266	56.71274897050173	3.8217497268678042	1.0421043785192032
8	89.92772501890916	2.720816875367678	5.859736112278343	1.4917219934448274
9	87.07664509622657	3.2292629632742242	7.082527943524666	2.6115639969745357
10	61.045045802168254	17.383813765862676	11.08916715690394	10.481973275065132
11	52.691402638877214	17.736784603748216	17.04134801243802	12.530464744936548
12	45.4302882595176	19.898730985797126	21.831666526598877	12.839314228086392
13	20.50382385074376	42.68005714765947	22.77712412807799	14.038994873518785
14	14.257500630305067	42.94898730985797	28.416253466677873	14.37725859315909
15	13.213295234893687	20.592066560215144	51.95814774350785	14.236490461383308
16	14.74703756618203	14.333137238423395	50.558870493318764	20.360954702075805
17	16.51399277250189	15.28489789057904	28.46667787209009	39.73443146482898
18	22.705689553744012	20.316833347340115	34.47558618371292	22.501890915202956
19	30.96058492310278	21.17614925624002	25.51685015547525	22.346415665181947
20	35.292881754769304	19.894528952012774	23.905370199176403	20.907219094041515
21	23.21413564165056	27.000168081351372	27.05059248676359	22.735103790234472
22	23.815026472812843	22.604840742919574	25.136566097991427	28.443566686276156
23	20.62358181359778	29.166316497184635	23.758299016724095	26.451802672493486
24	21.037482141356417	20.46810656357677	39.58736028237667	18.907051012690143
25	17.8334313807883	22.40524413816287	37.25943356584587	22.501890915202956
26	17.38801579964703	33.62467434238171	27.697705689553743	21.289604168417515
27	18.640221867383815	33.21077401462308	28.807042608622574	19.341961509370538
28	15.70720228590638	26.6240860576519	40.028573829733595	17.640137826708127
29	16.640053786032443	23.096478695688713	39.48861248844441	20.77485502983444
30	19.02260694175981	28.84906294646609	31.86192116984621	20.266408941927892
31	32.39557946045886	24.044037314060006	24.2877552735524	19.272627951928733
32	33.80956382889318	24.035633246491305	24.43902848978906	17.715774434826457
33	31.366081183292714	25.336162702748133	23.163711236238342	20.134044877720818
34	21.575342465753426	25.941255567694764	26.95184469283133	25.53155727372048
35	22.44096142532986	24.15329019245315	30.58450289940331	22.821245482813683
36	33.48390621060594	21.913606185393732	25.344566770316835	19.257920833683503
37	22.237162786788804	31.02571644676023	27.903605344986975	18.83351542146399
38	21.901000084040675	32.90822758214976	22.031263131355576	23.159509202453986
39	21.749726867804018	31.83250693335574	25.38868812505253	21.029078073787712
40	26.216488780569797	24.619715942516176	23.516682074123878	25.647113202790152
41	18.886040843768384	23.331792587612405	25.93285150012606	31.84931506849315
42	23.426338347760318	23.350701739641988	23.586015631565676	29.63694428103202
43	24.737372888478024	24.502058996554332	23.455752584250778	27.30481553071687
44	21.19085637448525	29.9163795276914	26.75855113875116	22.13421295907219
45	18.463736448441047	37.09975628204051	21.545928229262966	22.890579040255485
46	23.146903101100932	31.952264896209766	24.962181695940835	19.938650306748464
47	22.869568871333726	26.15345827380452	26.216488780569797	24.76048407429196
48	25.533658290612653	21.285402134633163	31.023615429868055	22.157324144886125
49	21.928313303638962	21.705605513068324	33.3998655349189	22.966215648373815
50	19.732750651315236	33.73182620388268	25.850911841331204	20.684511303470877
51	19.543659131019414	34.44617194722245	22.934700394991175	23.075468526766954
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15.0
1	36.0
2	57.0
3	217.0
4	377.0
5	287.5
6	198.0
7	192.0
8	186.0
9	210.0
10	234.0
11	227.0
12	220.0
13	203.5
14	187.0
15	184.0
16	181.0
17	164.5
18	148.0
19	138.0
20	128.0
21	134.5
22	141.0
23	156.0
24	171.0
25	197.5
26	220.5
27	217.0
28	268.5
29	320.0
30	389.0
31	458.0
32	551.5
33	645.0
34	725.5
35	806.0
36	1014.5
37	1223.0
38	1858.0
39	2493.0
40	3380.0
41	4267.0
42	4514.5
43	4762.0
44	4556.0
45	4350.0
46	4086.0
47	3822.0
48	3644.0
49	3466.0
50	3391.5
51	3317.0
52	3042.5
53	2768.0
54	2581.0
55	2394.0
56	2313.0
57	2232.0
58	2092.5
59	1953.0
60	1820.5
61	1688.0
62	1449.0
63	1210.0
64	1080.5
65	951.0
66	789.0
67	627.0
68	530.5
69	434.0
70	380.0
71	326.0
72	253.5
73	181.0
74	139.0
75	77.5
76	58.0
77	44.5
78	31.0
79	24.0
80	17.0
81	12.5
82	8.0
83	5.5
84	3.0
85	3.0
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	47596.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.17060257164468
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.947598918466056	35.280275653416254
2	15.857420313035531	17.49726867804017
3	9.128298868959215	15.108412471636273
4	5.148710918161392	11.362299352886797
5	2.749533493278495	7.584670980754685
6	1.4813968544118208	4.903773426338347
7	0.7045203549259302	2.720816875367678
8	0.44556152176396663	1.9665518110765612
9	0.22849308808408544	1.134549121774939
>10	0.2970410145093111	1.909824354987814
>50	0.007616436269469516	0.31725355071854777
>100	0.003808218134734758	0.21430372300193296
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	102	0.21430372300193296	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	86	0.18068745272711995	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	65	0.13656609799142785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006303050676527439	0.0
2	0.0	0.0	0.0	0.014707118245230692	0.0
3	0.0	0.0	0.0	0.025212202706109757	0.0
4	0.0	0.0	0.0	0.035717287166988826	0.0
5	0.0	0.0	0.0	0.06723254054962602	0.0
6	0.0	0.0	0.0	0.11345491217749391	0.0
7	0.0	0.0	0.0	0.1449701655601311	0.0
8	0.0	0.0	0.0	0.2899403311202622	0.0
9	0.0	0.0	0.0	0.33196066896377846	0.0
10	0.0	0.0	0.0	0.42230439532733843	0.0
11	0.0	0.0	0.0	0.5231532061517774	0.0
12	0.0	0.0	0.0	0.6155979494075132	0.0
13	0.0	0.0	0.0	0.6660223548197327	0.0
14	0.0	0.0	0.0	0.684931506849315	0.0
15	0.0	0.0	0.0	0.7143457433397764	0.0
16	0.0	0.0	0.0	0.7710731994285234	0.0
17	0.0	0.0	0.0	0.8509118413312043	0.0
18	0.0	0.0	0.0	0.9286494663417094	0.0
19	0.0	0.0	0.0	0.9748718379695773	0.0
20	0.0	0.0	0.0	1.0168921758130935	0.0
21	0.0	0.0	0.0	1.0820236994705437	0.0
22	0.0	0.0	0.0	1.1324481048827633	0.0
23	0.0	0.0	0.0	1.2101857298932683	0.0
24	0.0	0.0	0.0	1.2669131859820153	0.0
25	0.0	0.0	0.0	1.3194386082864107	0.0
26	0.0	0.0	0.0	1.3782670812673334	0.0
27	0.0	0.0	0.0	1.4286914866795528	0.0
28	0.0	0.0	0.0	1.4812169089839482	0.0
29	0.0	0.0	0.0	1.5274392806118162	0.0
30	0.0	0.0	0.0	1.5841667367005632	0.0
31	0.0	0.0	0.0	1.651399277250189	0.0
32	0.0	0.0	0.0	1.7018236826624087	0.0
33	0.0	0.0	0.0	1.7585511387511557	0.0
34	0.0	0.0	0.0	1.8278846961929573	0.0
35	0.0	0.0	0.0	1.9035213043112866	0.0
36	0.0	0.0	0.0	1.966551811076561	0.0
37	0.0	0.0	0.0	2.025380284057484	0.0
38	0.0	0.0	0.0	2.0926128246071096	0.0
39	0.0	0.0	0.0	2.1325321455584505	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACAT	25	3.8344868E-5	45.000004	22
CAACTAG	25	3.8344868E-5	45.000004	16
TACGGGG	25	3.8344868E-5	45.000004	4
CACAACT	25	3.8344868E-5	45.000004	14
GGCCGAT	30	2.1217638E-6	45.000004	8
AGGGCAA	25	3.8344868E-5	45.000004	7
CATCAAC	25	3.8344868E-5	45.000004	26
TACATCA	25	3.8344868E-5	45.000004	24
CCACAAC	25	3.8344868E-5	45.000004	13
TGCTAGG	25	3.8344868E-5	45.000004	3
AAGGATC	25	3.8344868E-5	45.000004	6
CTAGATA	25	3.8344868E-5	45.000004	19
TGGGATG	25	3.8344868E-5	45.000004	6
ATACATC	25	3.8344868E-5	45.000004	23
CCTAGGG	25	3.8344868E-5	45.000004	4
ACAACTA	25	3.8344868E-5	45.000004	15
TAGATAC	25	3.8344868E-5	45.000004	20
ATTTATC	25	3.8344868E-5	45.000004	36
GTACGGG	25	3.8344868E-5	45.000004	3
AACATGA	25	3.8344868E-5	45.000004	30
>>END_MODULE