FastQCFastQC Report
Thu 26 May 2016
SRR1546381_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546381_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1748667
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71290.4076819657487675No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA66630.3810330954950256No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31960.18276778826386042No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28550.16326722011680897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA302.5284771E-644.00000437
GTCGAAC302.5284771E-644.00000415
ATAATCG302.5284771E-644.00000440
TGACGTA302.5284771E-644.00000435
CGTTAGT302.5284771E-644.00000422
GCGATCG302.5284771E-644.0000049
ATACGAT302.5284771E-644.00000410
AACGTCA207.8576105E-444.020
TCACCGT254.4437962E-544.039
AATTTCG850.044.037
GTCGATA207.8576105E-444.01
CGTATAC207.8576105E-444.028
CGAAAAT207.8576105E-444.029
ACGTTGT408.3164196E-944.010
ACGTTGC254.4437962E-544.018
ACGTTCG207.8576105E-444.040
CGTTGAT254.4437962E-544.033
ATAGCCG254.4437962E-544.043
ATTCGGC1000.044.017
GCGCATT207.8576105E-444.029
Produced by FastQC (version 0.11.5)