##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546381_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1748667
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.603837094198038	31.0	31.0	33.0	30.0	34.0
2	31.784938470274785	31.0	31.0	34.0	30.0	34.0
3	31.81978615711282	31.0	31.0	34.0	30.0	34.0
4	35.691691442681766	37.0	35.0	37.0	35.0	37.0
5	27.052414210367097	35.0	25.0	37.0	0.0	37.0
6	31.31861698082025	35.0	26.0	37.0	17.0	37.0
7	15.06051009140105	0.0	0.0	32.0	0.0	37.0
8	25.168820593057454	17.0	17.0	35.0	17.0	37.0
9	33.36934647934684	32.0	32.0	37.0	28.0	39.0
10	36.23304951714649	37.0	35.0	39.0	33.0	39.0
11	37.17071231972697	39.0	37.0	39.0	34.0	39.0
12	36.91326993647161	39.0	35.0	39.0	33.0	39.0
13	36.27973822345821	39.0	35.0	39.0	32.0	39.0
14	37.579922306534066	40.0	35.0	41.0	33.0	41.0
15	37.927126205275215	40.0	35.0	41.0	33.0	41.0
16	38.034394198552384	40.0	35.0	41.0	33.0	41.0
17	38.0634117301922	40.0	35.0	41.0	34.0	41.0
18	38.03437475517065	40.0	36.0	41.0	34.0	41.0
19	37.95921293190756	40.0	36.0	41.0	34.0	41.0
20	37.961573015331105	40.0	36.0	41.0	34.0	41.0
21	37.808980783648344	40.0	35.0	41.0	34.0	41.0
22	37.728860326179884	39.0	35.0	41.0	33.0	41.0
23	37.62528200051811	39.0	35.0	41.0	33.0	41.0
24	37.495194911323885	39.0	35.0	41.0	33.0	41.0
25	37.316538254567625	39.0	35.0	41.0	33.0	41.0
26	37.05445233426376	39.0	35.0	40.0	33.0	41.0
27	36.8216384251547	38.0	35.0	40.0	32.0	41.0
28	36.90391137935353	38.0	35.0	40.0	32.0	41.0
29	36.938146027802894	38.0	35.0	40.0	33.0	41.0
30	36.73166817924739	38.0	35.0	40.0	32.0	41.0
31	36.47009407737436	38.0	35.0	40.0	31.0	41.0
32	36.19700549046788	38.0	35.0	40.0	31.0	41.0
33	35.980105989304995	38.0	35.0	40.0	30.0	41.0
34	35.77251986799087	38.0	35.0	40.0	30.0	41.0
35	35.58898463801284	38.0	35.0	40.0	29.0	41.0
36	35.36685944207788	38.0	35.0	40.0	29.0	41.0
37	35.13736520446717	38.0	34.0	40.0	27.0	41.0
38	35.021772584488644	38.0	34.0	40.0	27.0	41.0
39	34.99879451033273	37.0	34.0	40.0	27.0	41.0
40	34.754025780780445	37.0	34.0	40.0	26.0	41.0
41	34.60380221048376	37.0	34.0	40.0	25.0	40.0
42	34.588389327413395	37.0	34.0	40.0	25.0	41.0
43	34.45709102991021	37.0	33.0	40.0	24.0	41.0
44	34.22164597376173	36.0	33.0	40.0	24.0	40.0
45	34.01085912869632	36.0	33.0	39.0	23.0	40.0
46	33.99393538049269	36.0	33.0	39.0	23.0	40.0
47	33.88937859523855	36.0	33.0	39.0	23.0	40.0
48	33.85358618879409	36.0	33.0	39.0	23.0	40.0
49	33.75259040171742	36.0	33.0	39.0	24.0	40.0
50	33.75462681002158	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	5.0
13	5.0
14	14.0
15	21.0
16	70.0
17	229.0
18	591.0
19	1194.0
20	2324.0
21	4070.0
22	6459.0
23	10081.0
24	15225.0
25	22214.0
26	28732.0
27	31431.0
28	32659.0
29	36282.0
30	44052.0
31	59035.0
32	82796.0
33	163976.0
34	155860.0
35	184262.0
36	216251.0
37	412714.0
38	195480.0
39	42632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.071362929591512	22.744410456650694	30.452110093002272	16.732116520755525
2	30.813413874682833	24.73181000156119	31.419246774829055	13.035529348926925
3	28.106609205755014	22.921173671144935	35.58293259951723	13.38928452358282
4	25.56238551994176	26.26617875215807	33.44273094877413	14.728704779126042
5	17.493038983408503	47.69095545349686	24.696525982362566	10.119479580732065
6	21.13810119365208	38.85668340513088	29.155350904431778	10.849864496785266
7	38.92490680043713	55.76956618956039	4.2425458935291855	1.0629811164732907
8	88.1577224251387	2.5087681073640664	7.97104308596205	1.3624663815351923
9	84.73997622188787	3.605832328282057	9.67577017236558	1.9784212774644916
10	57.79602405718184	19.851578373698366	12.62481650308492	9.72758106603487
11	51.74793142433637	15.972166227189053	19.953713314198758	12.326189034275822
12	43.25917970659937	20.394792147389985	24.02447121149996	12.321556934510687
13	19.367552541450145	42.535256855650616	25.68991122952512	12.40727937337412
14	12.680458886683398	42.73329341721437	32.49452297092586	12.091724725176377
15	11.819860499454728	19.47735046180891	55.99442318062845	12.70836585810792
16	13.961034319284346	14.257202772168743	52.37818292447904	19.403579984067864
17	15.287530444618671	14.704057433462175	30.953863714475084	39.054548407444074
18	21.712481564528865	20.249367089331475	37.48872712757775	20.54942421856191
19	30.148850524428035	20.511452437771172	27.995381624974907	21.344315412825885
20	32.5707524645916	20.956877438643264	25.717761014532787	20.754609082232353
21	22.519896584083764	26.488462354467718	29.725671039711965	21.265970021736557
22	23.29780341254224	22.77031590348534	26.515111224721462	27.41676945925096
23	19.106553734930664	28.527558420213794	26.455751724027504	25.910136120828035
24	20.151578316511948	20.789721542180413	42.20529123040579	16.853408910901845
25	16.25518180419714	22.396259550846445	39.511982555855404	21.836576089101012
26	16.081392283379284	33.51850295110504	29.871267657021033	20.528837108494642
27	17.135509505240275	33.5933599707663	30.464862663960606	18.806267860032815
28	13.541686324497459	26.751862990495045	43.10500512676227	16.601445558245224
29	14.85851794538354	21.918295478784696	41.39032760382623	21.832858972005535
30	18.645059350922732	28.530417741056475	33.203177048574716	19.62134585944608
31	31.0750417317877	24.203121577750366	25.355370690932006	19.36646599952993
32	32.37774830771096	25.028721877864683	26.923936918807296	15.669592895617063
33	29.477882295485646	25.312995556043543	25.82166873395564	19.38745341451517
34	20.712977370762985	25.656114057164686	29.98741326965054	23.643495302421787
35	21.10064409061302	25.19084536964442	32.12555620938692	21.582954330355637
36	32.58281879854769	22.059660301246605	26.637547343204854	18.719973557000845
37	20.99364830468008	31.966978275452103	28.525728454874482	18.513644964993333
38	21.130209468126292	33.36261277876233	23.183144646750925	22.324033106360446
39	22.20234041129615	31.034954053573376	26.044238268349552	20.718467266780923
40	25.437947876868495	24.647231291034828	24.476472650310207	25.43834818178647
41	17.984213117763417	23.29357161769508	26.098107873025565	32.62410739151594
42	23.55234015395727	23.443514402685018	24.163377018037167	28.840768425320544
43	23.62245070101969	25.413815208956308	25.154303249275017	25.80943084074898
44	20.654875971239807	29.21350949037181	28.293094111114353	21.838520427274034
45	17.97923789949716	37.55500618471098	22.657544289450193	21.808211626341663
46	22.624376167675152	31.501023350929593	26.237471170897603	19.637129310497652
47	22.824128321744507	25.253693241766445	27.19076873984584	24.731409696643215
48	24.302454383824937	21.527769438091987	31.76385212278839	22.40592405529469
49	21.582554025437663	21.74284755187809	33.89959323301692	22.77500518966733
50	19.301044738649498	33.918293191328026	25.717475082448516	21.063186987573964
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	957.0
1	1582.5
2	2208.0
3	11864.5
4	21521.0
5	15662.5
6	9804.0
7	10424.5
8	11045.0
9	11919.0
10	12793.0
11	12919.5
12	13046.0
13	12328.0
14	11610.0
15	10935.5
16	10261.0
17	9334.5
18	8408.0
19	8238.5
20	8069.0
21	8188.5
22	8308.0
23	8197.0
24	8086.0
25	9136.0
26	10186.0
27	10683.5
28	11181.0
29	14137.5
30	17094.0
31	21119.0
32	25144.0
33	27708.5
34	30273.0
35	31907.0
36	33541.0
37	45141.0
38	56741.0
39	83040.0
40	109339.0
41	147457.0
42	185575.0
43	193144.5
44	200714.0
45	187821.0
46	174928.0
47	156061.0
48	137194.0
49	127336.0
50	117478.0
51	108714.0
52	99950.0
53	92531.5
54	85113.0
55	79398.0
56	73683.0
57	69114.5
58	64546.0
59	60744.5
60	56943.0
61	50822.0
62	44701.0
63	38104.0
64	31507.0
65	26220.0
66	20933.0
67	17457.0
68	13981.0
69	11430.5
70	8880.0
71	7490.0
72	6100.0
73	4831.0
74	3562.0
75	2669.0
76	1776.0
77	1363.0
78	950.0
79	623.5
80	297.0
81	209.0
82	121.0
83	90.0
84	59.0
85	46.0
86	33.0
87	25.0
88	17.0
89	10.5
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	2.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1748667.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.64723772903216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.87996371616126	13.131335080403675
2	6.383463540351758	2.1253407018169
3	2.046280013283463	1.021947295238907
4	1.0807548182020805	0.7196632954162787
5	0.7110012630051276	0.5918103525444239
6	0.5227633335220477	0.5221539293497715
7	0.4172080809968416	0.48617534747794094
8	0.3419989076577955	0.4554669695078912
9	0.29385088057994313	0.44026249193097783
>10	5.873880162059643	25.815650373146116
>50	2.289830119119915	26.770450460789675
>100	1.1568374496642155	26.660746785175775
>500	7.225717986659615E-4	0.0655570935723421
>1k	7.225717986659615E-4	0.36393208551031386
>5k	7.225717986659615E-4	0.8295077381190296
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7129	0.4076819657487675	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6663	0.3810330954950256	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3196	0.18276778826386042	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2855	0.16326722011680897	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014754095548209008	0.0
2	0.0	0.0	0.0	0.0481509629906666	0.0
3	0.0	0.0	0.0	0.06353410912426437	0.0
4	0.0	0.0	0.0	0.09361416438921762	0.0
5	0.0	0.0	0.0	0.15045746274161975	0.0
6	0.0	0.0	0.0	0.24647345663868536	0.0
7	0.0	0.0	0.0	0.3111512941000202	0.0
8	0.0	0.0	0.0	0.6335111259033309	0.0
9	0.0	0.0	0.0	0.7555469394687496	0.0
10	0.0	0.0	0.0	0.9780592874458087	0.0
11	0.0	0.0	0.0	1.212923901463229	0.0
12	0.0	0.0	0.0	1.4153066307078477	0.0
13	0.0	0.0	0.0	1.4974263253095073	0.0
14	0.0	0.0	0.0	1.5256192288182941	0.0
15	0.0	0.0	0.0	1.574456428811203	0.0
16	0.0	0.0	0.0	1.6919173290283398	0.0
17	5.718641685352328E-5	0.0	0.0	1.8201864620307926	0.0
18	5.718641685352328E-5	0.0	0.0	2.018680514929372	0.0
19	5.718641685352328E-5	0.0	0.0	2.1020011242849552	0.0
20	5.718641685352328E-5	0.0	0.0	2.1932706455831785	0.0
21	5.718641685352328E-5	0.0	0.0	2.312961816057603	0.0
22	5.718641685352328E-5	0.0	0.0	2.4306514619421535	0.0
23	5.718641685352328E-5	0.0	0.0	2.574418113911911	0.0
24	5.718641685352328E-5	0.0	0.0	2.6772964778313995	0.0
25	5.718641685352328E-5	0.0	0.0	2.7496372951511066	0.0
26	5.718641685352328E-5	0.0	0.0	2.8406780707819155	0.0
27	5.718641685352328E-5	0.0	0.0	2.909873635174679	0.0
28	5.718641685352328E-5	0.0	0.0	2.98844777193142	0.0
29	5.718641685352328E-5	0.0	0.0	3.0736555330431696	0.0
30	1.1437283370704656E-4	0.0	0.0	3.1921457887636695	0.0
31	1.1437283370704656E-4	0.0	0.0	3.311093535818998	0.0
32	1.1437283370704656E-4	0.0	0.0	3.4063089198801144	0.0
33	1.1437283370704656E-4	0.0	0.0	3.506270776540073	0.0
34	1.1437283370704656E-4	0.0	0.0	3.619042390575221	0.0
35	1.1437283370704656E-4	0.0	0.0	3.7706435816539114	0.0
36	1.7155925056056985E-4	0.0	0.0	3.8863888893654424	0.0
37	1.7155925056056985E-4	0.0	0.0	4.0038497895825795	0.0
38	1.7155925056056985E-4	0.0	0.0	4.111417439684057	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGTA	30	2.5284771E-6	44.000004	37
GTCGAAC	30	2.5284771E-6	44.000004	15
ATAATCG	30	2.5284771E-6	44.000004	40
TGACGTA	30	2.5284771E-6	44.000004	35
CGTTAGT	30	2.5284771E-6	44.000004	22
GCGATCG	30	2.5284771E-6	44.000004	9
ATACGAT	30	2.5284771E-6	44.000004	10
AACGTCA	20	7.8576105E-4	44.0	20
TCACCGT	25	4.4437962E-5	44.0	39
AATTTCG	85	0.0	44.0	37
GTCGATA	20	7.8576105E-4	44.0	1
CGTATAC	20	7.8576105E-4	44.0	28
CGAAAAT	20	7.8576105E-4	44.0	29
ACGTTGT	40	8.3164196E-9	44.0	10
ACGTTGC	25	4.4437962E-5	44.0	18
ACGTTCG	20	7.8576105E-4	44.0	40
CGTTGAT	25	4.4437962E-5	44.0	33
ATAGCCG	25	4.4437962E-5	44.0	43
ATTCGGC	100	0.0	44.0	17
GCGCATT	20	7.8576105E-4	44.0	29
>>END_MODULE