##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546380_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	679773
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.071164932999693	31.0	31.0	33.0	30.0	34.0
2	31.217540267118583	31.0	31.0	33.0	30.0	34.0
3	31.16213206467453	31.0	31.0	34.0	28.0	34.0
4	35.08891203387013	35.0	35.0	37.0	32.0	37.0
5	32.83342086255264	35.0	35.0	37.0	28.0	37.0
6	34.017701497411636	35.0	35.0	37.0	28.0	37.0
7	15.507472347386553	0.0	0.0	35.0	0.0	37.0
8	25.053545816029764	17.0	17.0	35.0	17.0	37.0
9	32.90408415750552	32.0	32.0	37.0	28.0	39.0
10	35.703742278672436	37.0	35.0	38.0	32.0	39.0
11	36.622656386764405	37.0	35.0	39.0	33.0	39.0
12	36.228424782979026	37.0	35.0	39.0	32.0	39.0
13	35.7079657473892	37.0	35.0	39.0	31.0	39.0
14	36.720309573931296	39.0	35.0	40.0	31.0	41.0
15	37.18942794138631	39.0	35.0	41.0	32.0	41.0
16	37.319018260507555	39.0	35.0	41.0	32.0	41.0
17	37.29838784417739	39.0	35.0	41.0	32.0	41.0
18	37.182438843555126	39.0	35.0	40.0	32.0	41.0
19	37.07698158061588	39.0	35.0	40.0	32.0	41.0
20	37.18212256150215	39.0	35.0	40.0	32.0	41.0
21	37.005351786552275	39.0	35.0	40.0	32.0	41.0
22	37.067228324749586	39.0	35.0	40.0	32.0	41.0
23	37.062291382564474	38.0	35.0	40.0	32.0	41.0
24	36.90337068403717	38.0	35.0	40.0	32.0	41.0
25	36.651126184770504	38.0	35.0	40.0	31.0	41.0
26	36.49025924830789	38.0	35.0	40.0	31.0	41.0
27	36.37238607594006	38.0	35.0	40.0	31.0	41.0
28	36.34168759276994	38.0	35.0	40.0	31.0	41.0
29	36.43937608584042	38.0	35.0	40.0	31.0	41.0
30	36.18394817093353	38.0	35.0	40.0	30.0	41.0
31	35.98664112872974	38.0	35.0	40.0	30.0	41.0
32	35.81464400616088	38.0	34.0	40.0	30.0	41.0
33	35.590410328153666	38.0	34.0	40.0	29.0	41.0
34	35.42787665882582	38.0	34.0	40.0	28.0	41.0
35	35.2114514698289	38.0	34.0	40.0	27.0	41.0
36	34.99823323374127	38.0	34.0	40.0	25.0	41.0
37	34.78420149079178	37.0	34.0	40.0	25.0	41.0
38	34.76529812157882	37.0	34.0	40.0	25.0	41.0
39	34.70469848022796	37.0	34.0	40.0	24.0	41.0
40	34.503082646707064	37.0	33.0	40.0	23.0	41.0
41	34.42229244174158	37.0	33.0	40.0	23.0	41.0
42	34.35445803231373	37.0	33.0	40.0	23.0	41.0
43	34.23418405850188	37.0	33.0	40.0	23.0	41.0
44	33.99337867199786	36.0	33.0	40.0	23.0	41.0
45	33.9077633268753	36.0	33.0	40.0	23.0	41.0
46	33.9147171188029	36.0	33.0	40.0	23.0	41.0
47	33.90263367329976	36.0	33.0	40.0	23.0	41.0
48	33.890482558148086	36.0	33.0	40.0	23.0	41.0
49	33.817334610230176	36.0	33.0	40.0	23.0	41.0
50	33.6356092401434	36.0	33.0	40.0	22.0	41.0
51	33.43614118242413	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	1.0
13	4.0
14	7.0
15	25.0
16	75.0
17	225.0
18	468.0
19	812.0
20	1441.0
21	2345.0
22	3591.0
23	5317.0
24	7666.0
25	10315.0
26	12381.0
27	13535.0
28	14388.0
29	16792.0
30	20138.0
31	26152.0
32	34971.0
33	54831.0
34	70158.0
35	70178.0
36	74566.0
37	114031.0
38	106164.0
39	19192.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.5545718938528	23.348088258874654	30.84470845414572	16.252631393126823
2	30.33071334107121	24.593062684160742	32.0174528850072	13.058771089760846
3	28.607196814230633	22.622993263927814	35.174977529263444	13.59483239257811
4	26.014566627388845	26.826161086127282	32.10851269467896	15.05075959180491
5	21.370663442060806	35.98892571490777	30.125644884395232	12.514765958636193
6	21.144558551163403	39.294588046303694	28.833301705128033	10.72755169740487
7	38.20245876196907	56.49356476353135	4.4987076568207325	0.8052688176788428
8	88.87834615378958	2.6619180226340267	7.220498607623426	1.2392372159529725
9	85.288471298507	3.7215364540809945	8.953577150019196	2.036415097392806
10	58.30608158900104	20.197477687404472	11.96061038022987	9.535830343364623
11	52.6352179330453	16.120381362601925	19.239510836705783	12.004889867646993
12	43.9414333902641	20.839750916850182	23.27659380410814	11.942221888777576
13	19.447668559945747	43.5798420943462	24.770769065555708	12.201720280152346
14	12.565076871249667	43.667518421590735	31.892999574858077	11.874405132301519
15	11.714204594769136	19.806317697231282	55.848349375453274	12.631128332546307
16	13.868306037456621	14.38980365504367	52.322466470424686	19.419423837075023
17	15.191836098226908	14.842160544770092	30.544166949849433	39.42183640715356
18	21.62692545893997	20.445354552181392	37.55739048182261	20.37032950705603
19	30.881926760845165	20.42784870831881	27.52183449475045	21.168390036085576
20	33.33907054266645	21.023782939304738	25.19797049897539	20.43917601905342
21	22.547821110870835	27.155682852952385	29.15223170087662	21.144264335300168
22	23.08447084541457	23.08299976609839	25.919093579768543	27.9134358087185
23	18.829668139217063	29.10103814067343	26.031925363319814	26.037368356789692
24	19.72025955723455	21.066591347405677	42.66615473106464	16.546994364295138
25	16.108759836004076	22.63108420016682	39.76489210368756	21.49526386014155
26	15.783945522990765	34.39780632652371	29.48351876288114	20.33472938760439
27	16.959779220416227	34.809267211260234	29.83981417326078	18.391139395062766
28	13.426246702943484	27.386936521456427	42.98361364749703	16.20320312810306
29	14.749923871645388	22.372909780176617	41.13402562325953	21.743140724918465
30	18.582085490303378	29.512351917478334	32.6732600441618	19.232302548056484
31	31.66733600775553	24.452133285670364	24.904637283328405	18.9758934232457
32	33.35010363753783	24.71192589290837	26.63462655916019	15.303343910393616
33	30.3763167998729	25.32610150741498	25.1082346606882	19.189347032023925
34	20.860198919345134	25.602075987130995	29.34582573888636	24.191899354637503
35	21.15117840808623	24.979662328453763	32.314316691013026	21.55484257244698
36	33.27081246239553	22.306564103016743	26.160350587622634	18.26227284696509
37	20.778112693502095	33.07530602127475	28.087464491823006	18.059116793400147
38	20.892121340506318	34.21877597374417	22.837770844090603	22.051331841658907
39	22.165487596594748	31.82238776768127	25.6734233339659	20.338701301758086
40	25.92733162393917	24.66441003099564	24.13349750578502	25.27476083928017
41	17.89420880205598	23.207894400042367	25.77478069885094	33.12311609905071
42	23.323668342226007	23.243053195699154	23.80279887550697	29.63047958656787
43	23.384571025916003	25.148983557746483	25.091611464415326	26.374833951922184
44	20.168497424875657	29.299192524563345	28.803438795009512	21.728871255551486
45	17.73209586141256	38.54051278882803	22.40674460444884	21.32064674531057
46	22.388503220928165	32.33035145555943	25.72888302418602	19.552262299326394
47	22.45911502810497	25.557502283850635	26.857053751767136	25.126328936277257
48	23.995657373858627	21.716661297227162	32.296957955082064	21.990723373832147
49	21.722251398628657	22.12047256951953	34.022386885033676	22.13488914681813
50	19.3358665319158	35.058909371216565	25.12162148246547	20.48360261440216
51	18.70698012424736	35.45595367865449	23.430174484717693	22.406891712380457
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	366.0
1	555.5
2	745.0
3	3897.5
4	7050.0
5	5268.0
6	3486.0
7	3719.5
8	3953.0
9	4098.5
10	4244.0
11	4225.0
12	4206.0
13	4166.0
14	4126.0
15	3827.0
16	3528.0
17	3292.0
18	3056.0
19	3025.5
20	2995.0
21	3124.5
22	3254.0
23	3210.5
24	3167.0
25	3607.5
26	4172.0
27	4296.0
28	4927.0
29	5558.0
30	7240.0
31	8922.0
32	10526.0
33	12130.0
34	12499.0
35	12868.0
36	15878.0
37	18888.0
38	27085.0
39	35282.0
40	49814.5
41	64347.0
42	70897.0
43	77447.0
44	73029.5
45	68612.0
46	63302.0
47	57992.0
48	53258.0
49	48524.0
50	44736.5
51	40949.0
52	38082.5
53	35216.0
54	32744.0
55	30272.0
56	28134.5
57	25997.0
58	24281.5
59	22566.0
60	20671.0
61	18776.0
62	16544.0
63	14312.0
64	12032.5
65	9753.0
66	8403.5
67	7054.0
68	5757.5
69	4461.0
70	3673.0
71	2885.0
72	2435.5
73	1986.0
74	1608.0
75	955.0
76	680.0
77	499.5
78	319.0
79	219.5
80	120.0
81	83.0
82	46.0
83	37.0
84	28.0
85	24.5
86	21.0
87	13.5
88	6.0
89	4.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	679773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.006555530455312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.46244767877232	17.591375485162093
2	5.38680703289887	2.478637502684702
3	2.2592301022170043	1.5593130840819525
4	1.486974925094485	1.3684068474632358
5	1.136773864462806	1.3076625519166918
6	0.897539339450114	1.2389573192296337
7	0.7767014391613555	1.25084573534552
8	0.6543208266295273	1.2042934746068514
9	0.5854217290191768	1.2121683765673368
>10	9.07433954013928	50.214944903381266
>50	1.202982753898771	17.403645073894243
>100	0.07454924904987087	2.2325897009434588
>500	0.0	0.0
>1k	0.0019115192064069456	0.9371599447230159
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2695	0.39645587571145074	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1924	0.2830356604337036	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1774	0.2609694706909512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013681037640506464	0.0
2	0.0	0.0	0.0	0.05413571883555246	0.0
3	0.0	0.0	0.0	0.06355062645912679	0.0
4	0.0	0.0	0.0	0.09635569521001863	0.0
5	0.0	0.0	0.0	0.15210960129337295	0.0
6	0.0	0.0	0.0	0.2186023863848667	0.0
7	0.0	0.0	0.0	0.25773309619534757	0.0
8	0.0	0.0	0.0	0.517084379638497	0.0
9	0.0	0.0	0.0	0.6224136586772349	0.0
10	0.0	0.0	0.0	0.7971778814398336	0.0
11	0.0	0.0	0.0	1.000481042936392	0.0
12	0.0	0.0	0.0	1.1615642280584841	0.0
13	0.0	0.0	0.0	1.228939660739688	0.0
14	0.0	0.0	0.0	1.2502703108243487	0.0
15	0.0	0.0	0.0	1.290872099951013	0.0
16	0.0	0.0	0.0	1.3872277951610317	0.0
17	0.0	0.0	0.0	1.4982942835328852	0.0
18	0.0	0.0	0.0	1.6665857573042766	0.0
19	0.0	0.0	0.0	1.7394041834553593	0.0
20	0.0	0.0	0.0	1.8223730568881082	0.0
21	0.0	0.0	0.0	1.924613069362861	0.0
22	0.0	0.0	0.0	2.0250877866581933	0.0
23	0.0	0.0	0.0	2.151453499918355	0.0
24	0.0	0.0	0.0	2.236040560598906	0.0
25	0.0	0.0	0.0	2.3032688853484915	0.0
26	0.0	0.0	0.0	2.3775583908157576	0.0
27	0.0	0.0	0.0	2.437872642779281	0.0
28	0.0	0.0	0.0	2.5098084213406535	0.0
29	0.0	0.0	0.0	2.580861552312316	0.0
30	0.0	0.0	0.0	2.6833957806503053	0.0
31	0.0	0.0	0.0	2.7859300089882946	0.0
32	0.0	0.0	0.0	2.8627203492930726	0.0
33	0.0	0.0	0.0	2.952603295511884	0.0
34	0.0	0.0	0.0	3.054990415918255	0.0
35	0.0	0.0	0.0	3.1938603033659767	0.0
36	0.0	0.0	0.0	3.297718503088531	0.0
37	0.0	0.0	0.0	3.407313912144201	0.0
38	0.0	0.0	0.0	3.5023456359696548	0.0
39	0.0	0.0	0.0	3.597818683589963	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	30	2.1641881E-6	45.000004	1
ATAGCGG	45	3.8380676E-10	45.000004	2
CGCGAAG	30	2.1641881E-6	45.000004	2
TCGATAA	45	3.8380676E-10	45.000004	1
TCGTGAA	30	2.1641881E-6	45.000004	1
CCAAATC	30	2.1641881E-6	45.000004	18
CTTAGCC	30	2.1641881E-6	45.000004	27
CGCTTAG	30	2.1641881E-6	45.000004	25
CTTAACG	20	7.03125E-4	45.0	1
AGCGTAA	20	7.03125E-4	45.0	1
CTCACGA	20	7.03125E-4	45.0	24
TCGTTAA	35	1.2109558E-7	45.0	1
CGTATAA	25	3.889118E-5	45.0	11
GATAACC	20	7.03125E-4	45.0	9
GATCGGA	20	7.03125E-4	45.0	10
GTCGAAC	20	7.03125E-4	45.0	15
CGACGTG	20	7.03125E-4	45.0	12
ACGCATG	20	7.03125E-4	45.0	25
GCGCGAC	80	0.0	45.0	9
ATAATCG	20	7.03125E-4	45.0	40
>>END_MODULE