FastQCFastQC Report
Thu 26 May 2016
SRR1546379_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546379_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences940217
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29740.3163099582330462No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA23900.25419663758472777No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12190.12965092101078793No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12120.12890641203041425No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGT207.855751E-444.027
TCGTTAA502.7284841E-1144.01
ATCTACG254.4422202E-544.01
CCCTATG254.4422202E-544.033
CGTGATA207.855751E-444.014
CTATACG207.855751E-444.01
CCGGACC207.855751E-444.017
CGACATA207.855751E-444.044
CGTTCAC302.5272238E-644.021
TCTAGAC207.855751E-444.043
ATGTACG551.8189894E-1244.01
GACTTAT207.855751E-444.08
CGTTACA207.855751E-444.038
TACATAA408.309144E-944.01
ACGATTG254.4422202E-544.026
CCACTCG207.855751E-444.020
ACAGGTT454.802132E-1044.05
CCGTAGG207.855751E-444.03
CCGTAGA408.309144E-944.019
CATAGTA207.855751E-444.01
Produced by FastQC (version 0.11.5)