FastQCFastQC Report
Thu 26 May 2016
SRR1546378_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546378_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences353389
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8840.2501492689359318No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8180.2314729660515749No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6520.18449923455455602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG351.2087366E-745.0000042
GCGATAT700.045.0000049
TAGGCGA700.045.0000046
ACTATTC207.026649E-445.017
GTACCCG207.026649E-445.026
ACACGTG207.026649E-445.042
ACACGGG207.026649E-445.03
ACGTTAG207.026649E-445.02
GGACTAT207.026649E-445.08
TTGTATA207.026649E-445.01
CACGATC207.026649E-445.019
CACCTTG207.026649E-445.042
CCACTCG207.026649E-445.032
GTTAATA453.8380676E-1045.01
GATATAC207.026649E-445.011
CGGGATC207.026649E-445.06
TGTTACG207.026649E-445.01
TTAACGA207.026649E-445.01
TTACGGG1100.045.03
ACGTAAG207.026649E-445.02