##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546378_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	353389
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.041311416031625	31.0	31.0	33.0	30.0	33.0
2	31.184021008011	31.0	31.0	33.0	30.0	34.0
3	31.15806943623033	31.0	31.0	34.0	28.0	34.0
4	35.1279213557864	35.0	35.0	37.0	33.0	37.0
5	32.888140264694144	35.0	35.0	37.0	28.0	37.0
6	34.05287657510562	35.0	35.0	37.0	28.0	37.0
7	14.564825164337316	0.0	0.0	35.0	0.0	37.0
8	24.531060672516688	17.0	17.0	35.0	17.0	37.0
9	32.602607891020945	32.0	32.0	37.0	27.0	39.0
10	35.56503173556619	37.0	35.0	37.0	32.0	39.0
11	36.51752601241125	37.0	35.0	39.0	32.0	39.0
12	36.22696801541644	37.0	35.0	39.0	32.0	39.0
13	35.686057007999686	37.0	35.0	39.0	31.0	39.0
14	36.748322669918984	39.0	35.0	40.0	31.0	41.0
15	37.20148618095074	39.0	35.0	41.0	32.0	41.0
16	37.31991940892331	39.0	35.0	41.0	32.0	41.0
17	37.3159747473747	39.0	35.0	41.0	32.0	41.0
18	37.20662499398679	39.0	35.0	41.0	32.0	41.0
19	37.10376950046549	39.0	35.0	40.0	32.0	41.0
20	37.21354654502545	39.0	35.0	40.0	32.0	41.0
21	37.030600839301734	39.0	35.0	40.0	32.0	41.0
22	37.094589814623546	39.0	35.0	41.0	32.0	41.0
23	37.08737114058445	39.0	35.0	40.0	32.0	41.0
24	36.92582960986335	38.0	35.0	40.0	32.0	41.0
25	36.64111786161992	38.0	35.0	40.0	31.0	41.0
26	36.50330372479053	38.0	35.0	40.0	31.0	41.0
27	36.42754584890871	38.0	35.0	40.0	31.0	41.0
28	36.380730583011925	38.0	35.0	40.0	31.0	41.0
29	36.45532826432062	38.0	35.0	40.0	31.0	41.0
30	36.20933588764789	38.0	35.0	40.0	30.0	41.0
31	36.07237916290547	38.0	35.0	40.0	30.0	41.0
32	35.946914023922645	38.0	34.0	40.0	30.0	41.0
33	35.74813307714728	38.0	34.0	40.0	29.0	41.0
34	35.60543197439649	38.0	34.0	40.0	29.0	41.0
35	35.455217904349034	38.0	34.0	40.0	28.0	41.0
36	35.238006842318235	38.0	34.0	40.0	27.0	41.0
37	35.074699552051705	38.0	34.0	40.0	26.0	41.0
38	35.030521606501615	38.0	34.0	40.0	26.0	41.0
39	34.960460002999525	38.0	34.0	40.0	26.0	41.0
40	34.80281502819839	37.0	34.0	40.0	24.0	41.0
41	34.6840252526253	37.0	34.0	40.0	24.0	41.0
42	34.66213436185054	37.0	33.0	40.0	24.0	41.0
43	34.55066230131668	37.0	33.0	40.0	24.0	41.0
44	34.33261929488467	37.0	33.0	40.0	23.0	41.0
45	34.27541887268704	37.0	33.0	40.0	23.0	41.0
46	34.25991471155016	36.0	33.0	40.0	23.0	41.0
47	34.229758141877646	36.0	33.0	40.0	23.0	41.0
48	34.24404551358418	36.0	33.0	40.0	23.0	41.0
49	34.15559624096959	36.0	33.0	40.0	24.0	41.0
50	34.01885740642748	36.0	33.0	40.0	24.0	41.0
51	33.807945352005866	36.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	3.0
14	5.0
15	13.0
16	42.0
17	93.0
18	204.0
19	413.0
20	692.0
21	1126.0
22	1705.0
23	2600.0
24	3720.0
25	5006.0
26	6012.0
27	6518.0
28	7160.0
29	8381.0
30	10401.0
31	13638.0
32	18435.0
33	29014.0
34	36133.0
35	35845.0
36	39747.0
37	61210.0
38	55829.0
39	9441.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.249637085478042	24.6600771387904	29.67579636038473	16.41448941534683
2	28.60954925025963	25.091612925133493	33.60347945182221	12.695358372784666
3	28.372699772771647	23.876238366219663	35.254068462798784	12.496993398209904
4	27.50057302292941	27.591973717348306	30.856648056391116	14.050805203331173
5	21.76751398600409	36.0260223153522	29.06825056807088	13.138213130572826
6	21.31645297391826	39.34672556304808	28.250737855451074	11.08608360758258
7	36.15958617840397	58.979198560226834	4.065491568781145	0.7957236925880545
8	88.79931180653614	2.9831149243468245	6.743560212683473	1.4740130564335618
9	84.9038877837171	4.663699209652819	7.959783694455686	2.4726293121744027
10	59.61447583258109	19.999773620571098	11.951418974557782	8.434331572290025
11	53.31914688912218	17.137771690686467	18.42360684684583	11.119474573345519
12	47.22671050881607	20.320949435324813	20.785876187430848	11.666463868428275
13	20.2666183723885	43.334116228858285	21.420870485498984	14.978394913254235
14	13.654075254181652	43.93317279258834	26.742484910396193	15.670267042833819
15	12.738370464275912	20.543367224220336	52.015484352936845	14.702777958566903
16	14.2519999207672	15.610276494174974	50.045700347209454	20.092023237848377
17	15.08988678198812	16.51975584978593	29.209454736848063	39.18090263137788
18	21.486520519880358	21.746573888830724	34.095571735396405	22.671333855892513
19	31.354116851401713	22.919785279111686	24.05281432076267	21.673283548723926
20	34.1040609639802	20.85350704181511	24.452374012773458	20.59005798143123
21	21.932205020529786	27.554621111579593	27.01102750793035	23.50214635996027
22	23.002979719232915	24.472182212802323	24.95210660207307	27.572731465891692
23	20.674950267269214	30.036871549482296	24.26872370107728	25.019454482171206
24	20.781065624566697	21.038006276369668	40.3971261131501	17.783801985913538
25	16.832725410241974	24.290229746822906	37.34213572012711	21.53490912280801
26	15.2958920622883	34.945060542348514	28.166411518185342	21.59263587717784
27	17.903783083231225	33.21891739697614	30.35521762137475	18.52208189841789
28	14.289918475108168	28.53399511586382	39.95002674107004	17.226059667957973
29	15.929754463211928	22.89686436193543	38.384330015931454	22.78905115892119
30	18.41653248969266	29.129938962446484	31.498716711612417	20.954811836248442
31	30.525285167336847	25.932329529215682	24.068660880785764	19.47372442266171
32	32.699942556219916	25.597287974441763	25.600400691589154	16.102368777749167
33	28.653976213181508	27.813825557671574	24.172512443794233	19.359685785352685
34	19.599930954274186	27.06054800800251	28.758959673334484	24.580561364388817
35	21.197037825172828	24.43058499274171	30.60932853031645	23.763048651769015
36	30.54481039307958	25.305541485445215	25.793672129013633	18.355975992461566
37	21.238352070947315	31.97326458944676	28.929027219296582	17.859356120309346
38	21.223071459496477	31.786218586317062	23.727959840289312	23.26275011389715
39	22.19961572091944	30.553582595949507	26.836432373390227	20.410369309740826
40	24.718652816018608	25.459479497098098	24.540661990044967	25.281205696838327
41	16.408829929624293	24.195999309542742	27.60980109737428	31.785369663458678
42	22.712365127380874	24.694600001697843	23.366318702619495	29.226716168301785
43	23.13371383942341	27.030835707959216	24.71072953600706	25.12472091661031
44	20.820399050338295	30.753645416241028	26.44875760139676	21.977197932023916
45	17.105229647781904	38.212847598538715	21.186850750872267	23.495072002807106
46	21.12912399650244	34.63548667332599	24.349088398337244	19.886300931834324
47	22.300637541066642	26.67909867030383	27.416246685663676	23.604017102965855
48	24.11337081799377	22.255361655286386	30.894000662159833	22.73726686456002
49	20.888878827580935	23.366318702619495	31.73217049766688	24.01263197213269
50	19.440333456898774	33.26674005133153	25.896391794877598	21.396534696892093
51	18.96776639906732	33.82193560071196	22.626057970112257	24.584240030108464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	106.0
1	278.5
2	451.0
3	1704.5
4	2958.0
5	2342.0
6	1726.0
7	1705.0
8	1684.0
9	1731.0
10	1778.0
11	1759.5
12	1741.0
13	1644.0
14	1547.0
15	1490.0
16	1433.0
17	1406.0
18	1379.0
19	1254.5
20	1130.0
21	1105.0
22	1080.0
23	1229.5
24	1379.0
25	1649.5
26	2377.0
27	2834.0
28	3528.5
29	4223.0
30	4915.0
31	5607.0
32	6511.5
33	7416.0
34	7814.5
35	8213.0
36	9436.0
37	10659.0
38	15333.5
39	20008.0
40	25861.5
41	31715.0
42	34307.5
43	36900.0
44	34319.5
45	31739.0
46	29192.0
47	26645.0
48	25591.5
49	24538.0
50	23406.0
51	22274.0
52	21300.5
53	20327.0
54	18553.0
55	16779.0
56	16000.5
57	15222.0
58	14489.5
59	13757.0
60	12350.5
61	10944.0
62	9500.5
63	8057.0
64	6688.5
65	5320.0
66	4451.5
67	3583.0
68	3005.5
69	2428.0
70	2065.5
71	1703.0
72	1324.5
73	946.0
74	769.0
75	435.0
76	278.0
77	216.0
78	154.0
79	131.0
80	108.0
81	81.5
82	55.0
83	40.5
84	26.0
85	17.5
86	9.0
87	8.5
88	8.0
89	7.5
90	7.0
91	5.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	353389.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.74075593750796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.93711867201671	17.48752790833897
2	6.278238883086902	3.23213229613825
3	3.610179739460232	2.787862666919457
4	2.542736217226406	2.6180780952434852
5	1.9930742593305117	2.5651619037378075
6	1.695157478150937	2.6180780952434852
7	1.4291210905293246	2.575066003752239
8	1.2532292640026383	2.5807254894747715
9	1.1795745616445885	2.732682681124766
>10	11.943055021161985	57.58837994391449
>50	0.11982630682130489	1.9032850484876438
>100	0.01539053482108503	0.6448983980825662
>500	0.0032979717473753645	0.6661214695420626
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	884	0.2501492689359318	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	818	0.2314729660515749	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	652	0.18449923455455602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009621125728305069	0.0
2	0.0	0.0	0.0	0.03141014576005478	0.0
3	0.0	0.0	0.0	0.03961640005772676	0.0
4	0.0	0.0	0.0	0.05659485722532393	0.0
5	0.0	0.0	0.0	0.0964942315691773	0.0
6	0.0	0.0	0.0	0.13412981162401771	0.0
7	0.0	0.0	0.0	0.1581826259447804	0.0
8	0.0	0.0	0.0	0.3457945776467292	0.0
9	0.0	0.0	0.0	0.41285948345873813	0.0
10	0.0	0.0	0.0	0.5331235550625515	0.0
11	0.0	0.0	0.0	0.656217369527631	0.0
12	0.0	0.0	0.0	0.7657284182586328	0.0
13	0.0	0.0	0.0	0.8141170211862848	0.0
14	0.0	0.0	0.0	0.8279827612064892	0.0
15	0.0	0.0	0.0	0.8506207040966187	0.0
16	0.0	0.0	0.0	0.9080644841803225	0.0
17	0.0	0.0	0.0	0.9847505157206364	0.0
18	0.0	0.0	0.0	1.0880361301568526	0.0
19	0.0	0.0	0.0	1.136141758798378	0.0
20	0.0	0.0	0.0	1.1836814388676502	0.0
21	0.0	0.0	0.0	1.2431060389542403	0.0
22	0.0	0.0	0.0	1.3084730990494893	0.0
23	0.0	0.0	0.0	1.3896867191678293	0.0
24	0.0	0.0	0.0	1.4510921392573057	0.0
25	0.0	0.0	0.0	1.499197767898831	0.0
26	0.0	0.0	0.0	1.54617149939585	0.0
27	0.0	0.0	0.0	1.5852219508813234	0.0
28	0.0	0.0	0.0	1.629365939517076	0.0
29	0.0	0.0	0.0	1.682282131022754	0.0
30	0.0	0.0	0.0	1.7601000597075744	0.0
31	0.0	0.0	0.0	1.8314095798114824	0.0
32	0.0	0.0	0.0	1.8939468970454654	0.0
33	0.0	0.0	0.0	1.9595969314268413	0.0
34	0.0	0.0	0.0	2.025246965808217	0.0
35	0.0	0.0	0.0	2.114666840224229	0.0
36	0.0	0.0	0.0	2.190503948906163	0.0
37	0.0	0.0	0.0	2.2657751090158436	0.0
38	0.0	0.0	0.0	2.3404803205532714	0.0
39	0.0	0.0	0.0	2.411223892084926	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAAG	35	1.2087366E-7	45.000004	2
GCGATAT	70	0.0	45.000004	9
TAGGCGA	70	0.0	45.000004	6
ACTATTC	20	7.026649E-4	45.0	17
GTACCCG	20	7.026649E-4	45.0	26
ACACGTG	20	7.026649E-4	45.0	42
ACACGGG	20	7.026649E-4	45.0	3
ACGTTAG	20	7.026649E-4	45.0	2
GGACTAT	20	7.026649E-4	45.0	8
TTGTATA	20	7.026649E-4	45.0	1
CACGATC	20	7.026649E-4	45.0	19
CACCTTG	20	7.026649E-4	45.0	42
CCACTCG	20	7.026649E-4	45.0	32
GTTAATA	45	3.8380676E-10	45.0	1
GATATAC	20	7.026649E-4	45.0	11
CGGGATC	20	7.026649E-4	45.0	6
TGTTACG	20	7.026649E-4	45.0	1
TTAACGA	20	7.026649E-4	45.0	1
TTACGGG	110	0.0	45.0	3
ACGTAAG	20	7.026649E-4	45.0	2
>>END_MODULE