##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546377_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1457746
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.141266036744398	31.0	31.0	33.0	30.0	34.0
2	31.466077766634243	31.0	31.0	34.0	30.0	34.0
3	31.51635470102473	31.0	31.0	34.0	30.0	34.0
4	35.36223457310121	37.0	35.0	37.0	33.0	37.0
5	35.2626308012507	37.0	35.0	37.0	33.0	37.0
6	35.30537418727268	37.0	35.0	37.0	32.0	37.0
7	35.78240516523455	37.0	35.0	37.0	35.0	37.0
8	35.712510958699255	37.0	35.0	37.0	35.0	37.0
9	37.48883481758825	39.0	37.0	39.0	35.0	39.0
10	36.98604695193813	39.0	37.0	39.0	32.0	39.0
11	36.633401154933715	39.0	35.0	39.0	32.0	39.0
12	35.577778982072324	37.0	35.0	39.0	31.0	39.0
13	35.05307508989906	37.0	35.0	39.0	30.0	39.0
14	35.8799324436493	38.0	35.0	40.0	29.0	41.0
15	36.3023091814349	38.0	35.0	40.0	31.0	41.0
16	36.50328520880867	38.0	35.0	40.0	32.0	41.0
17	36.461000064483116	37.0	35.0	40.0	32.0	41.0
18	36.36639784983118	37.0	35.0	40.0	31.0	41.0
19	36.22540209336881	37.0	35.0	40.0	31.0	41.0
20	35.99763950647095	36.0	34.0	40.0	31.0	41.0
21	35.82813261020782	36.0	34.0	40.0	30.0	41.0
22	35.708868348806995	36.0	34.0	40.0	30.0	41.0
23	35.70126963133495	35.0	34.0	40.0	30.0	41.0
24	35.66977031663952	35.0	34.0	40.0	30.0	41.0
25	35.58792341052556	35.0	34.0	40.0	30.0	41.0
26	35.445811547416355	35.0	34.0	40.0	30.0	41.0
27	35.30823339594141	35.0	34.0	40.0	29.0	41.0
28	35.3150884996426	36.0	34.0	40.0	29.0	41.0
29	35.297693150933014	36.0	34.0	40.0	29.0	41.0
30	35.13250525125776	36.0	34.0	40.0	29.0	41.0
31	34.84003454648478	35.0	34.0	40.0	28.0	41.0
32	34.59670271775742	35.0	34.0	40.0	27.0	41.0
33	34.32626054195998	35.0	33.0	40.0	25.0	41.0
34	34.10955475096485	35.0	33.0	40.0	24.0	41.0
35	33.87172731051912	35.0	33.0	40.0	23.0	41.0
36	33.60427125164466	35.0	33.0	40.0	22.0	41.0
37	33.40263255738654	35.0	33.0	39.0	22.0	41.0
38	33.36068149046542	35.0	32.0	39.0	22.0	41.0
39	33.36765184058128	35.0	33.0	39.0	22.0	41.0
40	33.09059328579876	35.0	32.0	39.0	20.0	41.0
41	33.04164923107318	35.0	32.0	39.0	20.0	41.0
42	32.91330176862087	35.0	32.0	39.0	20.0	41.0
43	32.80418879557893	35.0	32.0	39.0	19.0	41.0
44	32.623185383461866	35.0	31.0	39.0	18.0	41.0
45	32.59103780768392	35.0	31.0	39.0	18.0	41.0
46	32.525308935850276	35.0	31.0	38.0	18.0	40.0
47	32.444430648412	35.0	31.0	38.0	18.0	40.0
48	32.3872800885751	35.0	31.0	38.0	18.0	40.0
49	32.39883011169298	35.0	31.0	38.0	18.0	40.0
50	32.215457288169546	35.0	31.0	38.0	18.0	40.0
51	31.980718177240753	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	7.0
12	3.0
13	3.0
14	16.0
15	54.0
16	191.0
17	476.0
18	1074.0
19	2201.0
20	3831.0
21	6101.0
22	9209.0
23	13074.0
24	18756.0
25	25718.0
26	32750.0
27	36420.0
28	38230.0
29	41730.0
30	47994.0
31	56586.0
32	69935.0
33	90553.0
34	159360.0
35	231325.0
36	96977.0
37	114268.0
38	155997.0
39	204873.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.66450122312117	23.410799961035735	30.272146176357197	14.652552639485892
2	31.02515801792631	25.399418005605916	31.229377408684368	12.346046567783414
3	28.31583828732852	25.078854615275915	32.76188032757422	13.843426769821354
4	26.046032710774032	27.376442809652712	31.346064403538065	15.231460076035194
5	21.537839925473985	31.920238505199123	32.16911588164193	14.372805687684961
6	19.493519447146486	40.348661563811525	28.693956285937332	11.463862703104656
7	85.49184837413377	4.654308775328487	8.107036479606187	1.7468063709315615
8	87.05659284950876	3.268127643636134	7.470917430059833	2.2043620767952716
9	83.38866990545678	4.45777247888178	9.431752856807702	2.7218047588537373
10	54.26411734280183	20.026602714053066	13.59331461036422	12.115965332780883
11	47.76284757426877	18.66106989832248	19.518420904602035	14.057661622806716
12	42.430162730681474	18.562698851514597	25.592524349235052	13.414614068568872
13	21.05119821971729	41.623163431763835	24.87587000753218	12.4497683409867
14	14.16220658468622	44.59302237838416	29.09697574200169	12.147795294927922
15	11.684751664556101	21.612475698784287	53.31978273306872	13.382989903590886
16	14.14128387249905	15.701089215816747	50.6708987711165	19.4867281405677
17	14.381654965954288	16.258662345840772	29.679450329481266	39.68023235872367
18	20.85637689967937	20.836208777112063	36.58710090783991	21.720313415368658
19	29.547602943173914	22.72487799657828	25.970367951618456	21.75715110862935
20	31.667382383487936	21.53749693019223	24.461394509057133	22.333726177262704
21	21.731220665328525	27.185325838657764	28.930623030349594	22.152830465664113
22	21.394605095812302	23.218996999477277	25.983195975156164	29.403201929554257
23	18.400187687018178	30.44501579836268	24.902623639509216	26.25217287510993
24	20.664573938120906	23.157738042155493	38.73102721598961	17.446660803733984
25	17.784648354377236	22.29585949815674	37.13520736808745	22.784284779378574
26	15.80762355033044	33.67500236666744	28.460307900004526	22.057066182997588
27	18.179984716130246	32.255344895475616	29.232596076408374	20.332074311985764
28	14.946705393120613	26.726192354497975	40.47577561523064	17.85132663715078
29	15.320913245517396	22.805619085903857	39.46455692555493	22.408910743023817
30	18.822483477917277	27.847581128673994	33.95907105901851	19.370864334390216
31	29.8501247816835	24.73894629105482	25.56577071725801	19.845158210003664
32	30.747949231210374	25.065546398343745	25.781103155144997	18.40540121530088
33	28.621721479599326	25.222089444937595	25.02651353527981	21.129675540183268
34	19.224268150967315	26.293469507033464	29.235683033944188	25.24657930805504
35	19.800294427149858	24.298540349278955	31.56249442632667	24.338670797244514
36	32.165754527880715	21.049620441421208	27.670252568005676	19.1143724626924
37	21.01250835193511	29.887031074000546	29.338650217527608	19.761810356536735
38	20.558725594170728	31.653456775048603	23.759557563526158	24.028260067254514
39	20.494585476482186	29.1096665674267	26.695391378196202	23.700356577894915
40	24.23419443442136	24.509962640953912	25.31216000592696	25.943682918697768
41	17.568012534419577	22.12930098933559	27.891896119076986	32.41079035716785
42	23.193135155232806	24.67871631957831	24.26787657108989	27.860271954098998
43	22.8179669160471	24.60613851795855	26.23125016292276	26.344644403071594
44	20.285358354610477	28.148936783225608	29.362248292912486	22.203456569251433
45	17.473140039485617	35.41295945932968	23.22661149473228	23.887289006452427
46	23.424108177967902	29.506992301813895	26.657456100033887	20.411443420184312
47	22.314861436766076	26.3450559974097	27.284794470367263	24.05528809545696
48	24.19296640155418	21.712355924831897	30.75268256609862	23.341995107515302
49	21.129126747732457	20.976768243576043	33.802185017142904	24.091919991548597
50	20.0134316952336	30.6200805901714	27.043188593897703	22.323299120697296
51	18.98211348204694	32.94366782690537	24.112225312228606	23.96199337881908
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	897.0
1	1239.0
2	1581.0
3	6758.0
4	11935.0
5	9101.0
6	6267.0
7	6996.0
8	7725.0
9	8247.5
10	8770.0
11	8997.0
12	9224.0
13	8853.0
14	8482.0
15	8093.5
16	7705.0
17	7520.5
18	7336.0
19	6917.0
20	6498.0
21	6066.0
22	5634.0
23	6247.0
24	6860.0
25	6619.0
26	6257.5
27	6137.0
28	7303.5
29	8470.0
30	11311.5
31	14153.0
32	15743.0
33	17333.0
34	20771.5
35	24210.0
36	26299.0
37	28388.0
38	38330.0
39	48272.0
40	72533.0
41	96794.0
42	120350.5
43	143907.0
44	146723.0
45	149539.0
46	146857.0
47	144175.0
48	137628.5
49	131082.0
50	121480.0
51	111878.0
52	102452.5
53	93027.0
54	83429.0
55	73831.0
56	69454.0
57	65077.0
58	59314.5
59	53552.0
60	48516.5
61	43481.0
62	40759.5
63	38038.0
64	32451.0
65	26864.0
66	21359.5
67	15855.0
68	13063.0
69	10271.0
70	9345.0
71	8419.0
72	6832.0
73	5245.0
74	4037.0
75	1891.0
76	953.0
77	616.5
78	280.0
79	218.0
80	156.0
81	156.0
82	156.0
83	102.5
84	49.0
85	31.5
86	14.0
87	12.5
88	11.0
89	6.0
90	1.0
91	2.5
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1457746.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.653761673255193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.56625310730514	17.053037139319464
2	7.247514804380353	2.9937688698612113
3	2.4038066604528288	1.4894294962072858
4	1.1836686697985879	0.9778884242447612
5	0.7163198816745563	0.7397350058960322
6	0.4725944052085507	0.5856511327974719
7	0.34581366115613926	0.499964705861231
8	0.27674937855653325	0.45727325663424956
9	0.22986301662051536	0.42727823664980596
>10	2.3524233102742027	10.991303221243479
>50	0.8022713297721192	12.228546311054474
>100	1.3916951386480032	49.20086285807122
>500	0.009690074194667954	1.349471996149283
>1k	6.682809789426174E-4	0.15417410450023003
>5k	6.682809789426174E-4	0.8516152415097755
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6799	0.46640498413303827	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5541	0.3801073712430012	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07820292424057415	0.0
2	0.0	0.0	0.0	0.3495807911666367	0.0
3	0.0	0.0	0.0	0.5261547622150909	0.0
4	0.0	0.0	0.0	0.8119384309749436	0.0
5	0.0	0.0	0.0	1.3366526130066554	0.0
6	0.0	0.0	0.0	1.870490469533101	0.0
7	0.0	0.0	0.0	2.1677301807036344	0.0
8	0.0	0.0	0.0	2.9394695646566684	0.0
9	0.0	0.0	0.0	3.255162421985723	0.0
10	0.0	0.0	0.0	3.7912640473717643	0.0
11	0.0	0.0	0.0	4.252181106996692	0.0
12	0.0	0.0	0.0	4.678455643164173	0.0
13	0.0	0.0	0.0	4.85468661893087	0.0
14	0.0	0.0	0.0	4.921502099817115	0.0
15	0.0	0.0	0.0	5.020970731526617	0.0
16	0.0	0.0	0.0	5.255510905191988	0.0
17	0.0	0.0	0.0	5.530524522104674	0.0
18	0.0	0.0	0.0	6.0259469070743465	0.0
19	0.0	0.0	0.0	6.178236812174411	0.0
20	0.0	0.0	0.0	6.3916484764835575	0.0
21	0.0	0.0	0.0	6.577003126744988	0.0
22	0.0	0.0	0.0	6.7452766119749255	0.0
23	0.0	0.0	0.0	6.947849625380553	0.0
24	0.0	0.0	0.0	7.077501841884663	0.0
25	0.0	0.0	0.0	7.1811550160316	0.0
26	0.0	0.0	0.0	7.293108675997053	0.0
27	0.0	0.0	0.0	7.408286491611022	0.0
28	6.859905635138084E-5	0.0	0.0	7.545759000539189	0.0
29	6.859905635138084E-5	0.0	0.0	7.668757108577214	0.0
30	6.859905635138084E-5	0.0	0.0	7.838745570215936	6.859905635138084E-5
31	6.859905635138084E-5	0.0	0.0	8.005235479980737	6.859905635138084E-5
32	6.859905635138084E-5	0.0	0.0	8.136465474780929	6.859905635138084E-5
33	6.859905635138084E-5	0.0	0.0	8.267558271468417	6.859905635138084E-5
34	6.859905635138084E-5	0.0	0.0	8.414909044511184	6.859905635138084E-5
35	6.859905635138084E-5	0.0	0.0	8.601704274955994	6.859905635138084E-5
36	6.859905635138084E-5	0.0	0.0	8.746379684801056	6.859905635138084E-5
37	6.859905635138084E-5	0.0	0.0	8.8926328729422	6.859905635138084E-5
38	6.859905635138084E-5	0.0	0.0	9.018580740403335	6.859905635138084E-5
39	6.859905635138084E-5	0.0	0.0	9.16737209362948	6.859905635138084E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATTA	30	2.1659034E-6	45.000004	19
GGTACGT	30	2.1659034E-6	45.000004	9
GTCGATG	65	0.0	45.000004	1
CGTTCCA	30	2.1659034E-6	45.000004	38
TTACGTA	30	2.1659034E-6	45.000004	36
ACGTAAG	30	2.1659034E-6	45.000004	1
TAGCTAC	30	2.1659034E-6	45.000004	44
GACTAAC	30	2.1659034E-6	45.000004	28
GTCATCG	20	7.0339104E-4	45.0	43
AGGTACG	105	0.0	45.0	1
GCGACTT	20	7.0339104E-4	45.0	24
CGAGTTA	25	3.8913226E-5	45.0	27
GATCGGT	25	3.8913226E-5	45.0	27
CGTGACC	20	7.0339104E-4	45.0	36
ATGTGCG	90	0.0	45.0	1
CGTTGAT	140	0.0	45.0	25
ATTACGT	35	1.2122291E-7	45.0	30
TATGCGT	20	7.0339104E-4	45.0	1
TCGATAT	25	3.8913226E-5	45.0	34
ACGGGTA	105	0.0	45.0	5
>>END_MODULE