FastQCFastQC Report
Thu 26 May 2016
SRR1546376_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546376_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1670262
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86940.5205171404246759No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC67690.4052657607010158No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCG45950.27510654017154196No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGC29250.17512222633335367No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTC26260.15722084319705532No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG20530.1229148480897009No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG16840.10082250569072397No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGC253.8915674E-545.016
GCGCGTT207.034207E-445.011
AACCACG207.034207E-445.01
ACTTACG253.8915674E-545.01
TTATACG406.8175723E-945.01
TAACCGA207.034207E-445.020
CGGATGT253.8915674E-545.045
CGCTAAG253.8915674E-545.035
TTGCGTA253.8915674E-545.015
TACCTCG207.034207E-445.021
GTCCGAT207.034207E-445.09
CTACCGT406.8175723E-945.038
ACATGCG207.034207E-445.01
ACCCGTG253.8915674E-545.014
CACGTTA207.034207E-445.043
CGCAATA253.8915674E-545.042
TATCGGC253.8915674E-545.044
AGTACGG2050.045.02
GCCCGTA207.034207E-445.09
CATGCGT302.1660962E-644.99999625