##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546376_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1670262
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.152479072145567	31.0	31.0	33.0	30.0	34.0
2	31.482242306895564	31.0	31.0	34.0	30.0	34.0
3	31.54738837380004	31.0	31.0	34.0	30.0	34.0
4	35.408996911861735	37.0	35.0	37.0	33.0	37.0
5	35.293143231421176	37.0	35.0	37.0	33.0	37.0
6	35.35626207145945	37.0	35.0	37.0	33.0	37.0
7	35.756100539915295	37.0	35.0	37.0	35.0	37.0
8	35.65293708412213	37.0	35.0	37.0	35.0	37.0
9	37.435477188608736	39.0	37.0	39.0	35.0	39.0
10	36.9516962009553	39.0	37.0	39.0	32.0	39.0
11	36.6149610061176	39.0	35.0	39.0	32.0	39.0
12	35.67404993947057	37.0	35.0	39.0	31.0	39.0
13	35.22438216279841	37.0	35.0	39.0	30.0	39.0
14	36.12007996350273	38.0	35.0	40.0	30.0	41.0
15	36.44748488560477	38.0	35.0	40.0	31.0	41.0
16	36.60894817699259	38.0	35.0	40.0	32.0	41.0
17	36.517259567660645	38.0	35.0	40.0	31.0	41.0
18	36.45657926720479	38.0	35.0	40.0	31.0	41.0
19	36.32318582354146	37.0	35.0	40.0	31.0	41.0
20	36.089236898163286	36.0	35.0	40.0	31.0	41.0
21	35.97896916771141	36.0	34.0	40.0	31.0	41.0
22	35.83415116909802	36.0	34.0	40.0	30.0	41.0
23	35.80724760546549	36.0	34.0	40.0	30.0	41.0
24	35.81247971875071	36.0	34.0	40.0	30.0	41.0
25	35.774041437810354	36.0	34.0	40.0	30.0	41.0
26	35.62156356308172	36.0	34.0	40.0	30.0	41.0
27	35.48867962032304	36.0	34.0	40.0	30.0	41.0
28	35.44129723360766	36.0	34.0	40.0	29.0	41.0
29	35.38324765815184	36.0	34.0	40.0	29.0	41.0
30	35.2404383264422	36.0	34.0	40.0	29.0	41.0
31	35.001654830200295	36.0	34.0	40.0	28.0	41.0
32	34.74877414441567	35.0	34.0	40.0	27.0	41.0
33	34.53659904853251	35.0	33.0	40.0	25.0	41.0
34	34.28999342618104	35.0	33.0	40.0	24.0	41.0
35	34.102871286061706	35.0	33.0	40.0	23.0	41.0
36	33.8452362563478	35.0	33.0	40.0	23.0	41.0
37	33.683916654991855	35.0	33.0	40.0	23.0	41.0
38	33.622811271525066	35.0	33.0	40.0	23.0	41.0
39	33.61212252927984	35.0	33.0	40.0	23.0	41.0
40	33.38380924669303	35.0	32.0	40.0	22.0	41.0
41	33.30125692855373	35.0	32.0	39.0	21.0	41.0
42	33.191450802329214	35.0	32.0	39.0	21.0	41.0
43	33.0811573274133	35.0	32.0	39.0	21.0	41.0
44	32.84444536246409	35.0	32.0	39.0	19.0	41.0
45	32.861037968893505	35.0	32.0	39.0	20.0	41.0
46	32.77856168672939	35.0	32.0	39.0	20.0	41.0
47	32.72826778074338	35.0	32.0	39.0	20.0	41.0
48	32.684858423409025	35.0	32.0	39.0	20.0	41.0
49	32.7258657623774	35.0	32.0	39.0	20.0	40.0
50	32.5453916810656	35.0	31.0	38.0	18.0	40.0
51	32.316007309032955	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	0.0
11	1.0
12	4.0
13	4.0
14	27.0
15	70.0
16	176.0
17	512.0
18	1165.0
19	2433.0
20	4131.0
21	6709.0
22	10117.0
23	14483.0
24	20019.0
25	27531.0
26	34777.0
27	39367.0
28	42157.0
29	46535.0
30	54052.0
31	64778.0
32	79478.0
33	102340.0
34	177951.0
35	248251.0
36	114272.0
37	138072.0
38	187224.0
39	253574.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.392089384779155	23.108889503562914	28.051527245426165	14.447493866231763
2	30.87054605804359	26.881650902672753	29.550633373686285	12.697169665597375
3	28.372854079180392	26.223251202505953	32.02270063020053	13.381194088113121
4	26.464410972649798	26.135839766455803	32.60416629247388	14.795582968420524
5	22.657523190972434	32.236619165136965	30.502220609700753	14.603637034189845
6	20.222755471896026	41.77488322191369	27.36127625486301	10.641085051327277
7	84.56637341925997	4.742788855880095	8.888665371061547	1.8021723537983863
8	85.67997116620027	4.637416165847035	7.407460625937727	2.275152042014965
9	82.09646151322367	4.752428062184256	8.975597840338821	4.17551258425325
10	52.406867904556286	20.83553358694624	14.358346175629933	12.399252332867539
11	45.98739598937173	19.15268383044097	19.994048837846996	14.865871342340304
12	40.4871810530324	18.86039435729245	25.373264793188135	15.279159796487018
13	23.050156203038803	37.291514744393396	25.95688580593943	13.701443246628372
14	16.760364541610837	40.642845254217605	27.4865859368171	15.110204267354463
15	14.63351258664808	21.23415368367358	48.356724873103744	15.775608856574596
16	15.966776469799349	16.342046936348908	45.36192525484026	22.329251339011485
17	16.506931247912004	17.6760891405061	29.344498048809108	36.472481562772785
18	21.773889365859965	21.589247674915672	34.75688245317202	21.879980506052345
19	28.861160704129052	23.657366329354318	25.948384145720848	21.53308882079578
20	30.52233721416161	21.81106916160459	25.763323358850286	21.903270265383515
21	22.857372076955592	27.810127991895882	27.467726620135046	21.864773311013483
22	22.086295443469346	22.940293199510016	25.97137455081897	29.002036806201666
23	20.168871709947304	29.297319821680674	24.60033216345699	25.933476304915036
24	21.316715581148348	23.16612603292178	36.86212103250867	18.6550373534212
25	18.835009118329936	23.471527221477828	34.72568974208837	22.967773918103866
26	17.18407052306764	31.595881364720025	27.7009235676798	23.519124544532534
27	19.259253937406225	30.801874196982272	28.62664659795888	21.31222526765262
28	16.05628338548084	26.96115938697043	38.190894602164214	18.791662625384518
29	17.503960456503233	23.92451004692677	36.337712287054366	22.23381720951563
30	19.85059828937017	28.60994263175478	32.2430253457242	19.29643373315085
31	29.5587758088252	24.053531721370657	24.079994635572145	22.307697834231995
32	29.339768251926944	25.59251183347283	25.597481113741438	19.470238800858787
33	27.780132697744424	25.555691262807873	25.241968026573076	21.422208012874627
34	18.58103698701162	26.7102406688292	29.57446197063694	25.13426037352224
35	20.996945389405973	23.78333459062111	30.951192088426843	24.268527931546068
36	30.0905486684125	23.88697102610249	26.7886714778879	19.233808827597105
37	20.853674453468976	30.148982614703563	27.71247864107547	21.28486429075199
38	20.73997971575717	31.058660258091248	23.768726104048348	24.43263392210324
39	20.55605647497219	27.924241825533958	27.10299342258879	24.416708276905062
40	23.28550850106151	23.948817610650305	25.70249457869484	27.063179309593345
41	18.044115234615887	22.20298372351164	27.621774308461788	32.13112673341069
42	21.73790698704754	24.183451458513694	24.88112643405645	29.197515120382313
43	22.513114708949853	24.46370689149367	25.91701182209737	27.106166577459106
44	20.20263886743517	27.757262034339526	28.39033636639042	23.649762731834887
45	18.838960594206178	33.06020253109991	24.119030427561665	23.981806447132247
46	23.138884797714372	29.296840854907792	26.604089657790215	20.96018468958762
47	22.139460755258757	25.941738481747173	27.814917659624655	24.103883103369412
48	22.853839697005622	23.095717917308782	30.169578185937297	23.880864199748302
49	21.598288172753737	21.43352360288386	32.56141850799455	24.40676971636785
50	20.59509226696171	28.27568369513286	28.234552423512	22.89467161439343
51	19.242190746122464	29.70965034228163	25.4694173728433	25.578741538752602
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	895.0
1	1458.5
2	2022.0
3	8985.0
4	15948.0
5	11895.0
6	7842.0
7	8160.0
8	8478.0
9	8796.0
10	9114.0
11	9118.0
12	9122.0
13	8808.5
14	8495.0
15	7927.0
16	7359.0
17	6963.5
18	6568.0
19	6132.5
20	5697.0
21	5809.0
22	5921.0
23	5429.0
24	4937.0
25	5612.0
26	8317.5
27	10348.0
28	10830.0
29	11312.0
30	15153.5
31	18995.0
32	20728.0
33	22461.0
34	25542.0
35	28623.0
36	30157.0
37	31691.0
38	38241.0
39	44791.0
40	71321.0
41	97851.0
42	121309.5
43	144768.0
44	150347.0
45	155926.0
46	160800.0
47	165674.0
48	155241.5
49	144809.0
50	137703.5
51	130598.0
52	120457.0
53	110316.0
54	100230.5
55	90145.0
56	83721.0
57	77297.0
58	74097.0
59	70897.0
60	66485.5
61	62074.0
62	54083.5
63	46093.0
64	42523.0
65	38953.0
66	32599.0
67	26245.0
68	20789.0
69	15333.0
70	12976.0
71	10619.0
72	8793.5
73	6968.0
74	5629.5
75	3283.5
76	2276.0
77	1762.0
78	1248.0
79	825.5
80	403.0
81	352.5
82	302.0
83	248.0
84	194.0
85	116.5
86	39.0
87	31.0
88	23.0
89	14.0
90	5.0
91	5.5
92	6.0
93	3.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1670262.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.39812224823601
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.76953846317781	16.679450417537375
2	7.509498682567632	3.063593442999636
3	2.459155803441048	1.504864821181486
4	1.2146647773331476	0.9910752247467165
5	0.7515376604489419	0.7664978535995399
6	0.5025090683789052	0.6150144844584057
7	0.377069561099171	0.5384057702372711
8	0.31367311339892967	0.5118674010476931
9	0.2346745351481759	0.4308227870850405
>10	2.5662684091335604	12.064829423741939
>50	0.948021645912535	14.265390361955015
>100	1.3383309496202014	44.76547803176628
>500	0.010038220226573918	1.3097467120233464
>1k	0.004428626570547317	1.5617229968819855
>5k	5.904835427396423E-4	0.931240270738321
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8694	0.5205171404246759	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6769	0.4052657607010158	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCG	4595	0.27510654017154196	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGC	2925	0.17512222633335367	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTC	2626	0.15722084319705532	No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2053	0.1229148480897009	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	1684	0.10082250569072397	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11956208067955806	0.0
2	0.0	0.0	0.0	0.3752105957029496	0.0
3	0.0	0.0	0.0	0.5181223065602881	0.0
4	0.0	0.0	0.0	0.7805960980971848	0.0
5	0.0	0.0	0.0	1.366312590479817	0.0
6	0.0	0.0	0.0	1.7817563951044806	0.0
7	0.0	0.0	0.0	2.092905184935058	0.0
8	0.0	0.0	0.0	2.8102177981657968	0.0
9	0.0	0.0	0.0	3.117894078893012	0.0
10	0.0	0.0	0.0	3.9336942347966968	0.0
11	0.0	0.0	0.0	4.378175400027062	0.0
12	0.0	0.0	0.0	5.020948809228732	0.0
13	0.0	0.0	0.0	5.2089432675831695	0.0
14	0.0	0.0	0.0	5.280069833355485	0.0
15	0.0	0.0	0.0	5.415797042619661	0.0
16	0.0	0.0	0.0	5.66408144351006	0.0
17	0.0	0.0	0.0	5.934937153572314	0.0
18	0.0	0.0	0.0	6.408216196021941	0.0
19	0.0	0.0	0.0	6.591959824267091	0.0
20	0.0	0.0	0.0	6.765106312662325	0.0
21	0.0	0.0	0.0	6.943042468786334	0.0
22	0.0	0.0	0.0	7.113554639930741	0.0
23	0.0	0.0	0.0	7.320528156660452	0.0
24	0.0	0.0	0.0	7.470803981650783	0.0
25	0.0	0.0	0.0	7.582403239731252	0.0
26	0.0	0.0	0.0	7.69801384453457	0.0
27	0.0	0.0	0.0	7.821228046857319	0.0
28	0.0	0.0	0.0	7.944083024100411	0.0
29	0.0	0.0	0.0	8.062627300387604	0.0
30	0.0	0.0	0.0	8.230145929201527	0.0
31	0.0	0.0	0.0	8.38395413414183	0.0
32	0.0	0.0	0.0	8.513275162818767	0.0
33	0.0	0.0	0.0	8.652534752032915	0.0
34	0.0	0.0	0.0	8.796584008975838	0.0
35	0.0	0.0	0.0	8.978950607748963	0.0
36	0.0	0.0	0.0	9.120365547441061	0.0
37	0.0	0.0	0.0	9.268306409413613	0.0
38	0.0	0.0	0.0	9.41924081371665	0.0
39	0.0	0.0	0.0	9.56795999669513	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGC	25	3.8915674E-5	45.0	16
GCGCGTT	20	7.034207E-4	45.0	11
AACCACG	20	7.034207E-4	45.0	1
ACTTACG	25	3.8915674E-5	45.0	1
TTATACG	40	6.8175723E-9	45.0	1
TAACCGA	20	7.034207E-4	45.0	20
CGGATGT	25	3.8915674E-5	45.0	45
CGCTAAG	25	3.8915674E-5	45.0	35
TTGCGTA	25	3.8915674E-5	45.0	15
TACCTCG	20	7.034207E-4	45.0	21
GTCCGAT	20	7.034207E-4	45.0	9
CTACCGT	40	6.8175723E-9	45.0	38
ACATGCG	20	7.034207E-4	45.0	1
ACCCGTG	25	3.8915674E-5	45.0	14
CACGTTA	20	7.034207E-4	45.0	43
CGCAATA	25	3.8915674E-5	45.0	42
TATCGGC	25	3.8915674E-5	45.0	44
AGTACGG	205	0.0	45.0	2
GCCCGTA	20	7.034207E-4	45.0	9
CATGCGT	30	2.1660962E-6	44.999996	25
>>END_MODULE