FastQCFastQC Report
Thu 26 May 2016
SRR1546375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546375_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences876910
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC46230.5271920721624795No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33900.38658471222816476No Hit
ACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCT14250.16250242328175069No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG207.0323696E-445.0000041
GTCATAG207.0323696E-445.0000041
CTAGAGT207.0323696E-445.00000427
CGAACGG207.0323696E-445.0000042
TCCGCTA207.0323696E-445.00000422
CGAGTCA207.0323696E-445.00000413
TTTCGAT207.0323696E-445.00000436
CGTTGAT406.8121153E-945.00000425
GACCGAC207.0323696E-445.0000049
CCGTGAC207.0323696E-445.00000445
ATTCGGC207.0323696E-445.00000417
TGTATCG207.0323696E-445.0000041
CACTGAT207.0323696E-445.00000433
TAACGCG207.0323696E-445.0000041
GACCCTA207.0323696E-445.00000438
ACTTAAC1350.045.00000435
TCGATAG351.2114833E-745.0000041
GTGGACG207.0323696E-445.0000041
CCCGTTC207.0323696E-445.00000417
TCTACCA207.0323696E-445.00000427